SH3 containing Grb 2 like 1 protein (SH3GL1) (NM_003025) Human Untagged Clone

CAT#: SC118256

SH3GL1 (untagged)-Human SH3-domain GRB2-like 1 (SH3GL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SH3GL1
• Synonyms: CNSA1; EEN; SH3D2B; SH3P8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118256 sequence for NM_003025 edited (data generated by NextGen Sequencing)
  ATGTCGGTGGCGGGGCTGAAGAAGCAGTTCTACAAGGCGAGCCAGCTGGTCAGTGAGAAG
  GTCGGAGGGGCCGAGGGGACCAAGCTGGATGATGACTTCAAAGAGATGGAGAAGAAGGTG
  GATGTCACCAGCAAGGCGGTGACAGAAGTGCTGGCCAGGACCATCGAGTACCTGCAGCCC
  AACCCAGCCTCGCGGGCTAAGCTGACCATGCTCAACACGGTGTCCAAGATCCGGGGCCAG
  GTGAAGAACCCCGGCTACCCGCAGTCGGAGGGGCTTCTGGGCGAGTGCATGATCCGCCAC
  GGGAAGGAGCTGGGCGGCGAGTCCAACTTTGGTGACGCATTGCTGGATGCCGGCGAGTCC
  ATGAAGCGCCTGGCAGAGGTGAAGGACTCCCTGGACATCGAGGTCAAGCAGAACTTCATT
  GACCCCCTCCAGAACCTGTGCGAGAAAGACCTGAAGGAGATCCAGCACCACCTGAAGAAA
  CTGGAGGGCCGCCGCCTGGACTTTGACTACAAGAAGAAGCGGCAGGGCAAGATCCCCGAT
  GAGGAGCTACGCCAGGCGCTGGAGAAGTTCGAGGAGTCCAAGGAGGTGGCAGAAACCAGC
  ATGCACAACCTCCTGGAGACTGACATCGAGCAGGTGAGTCAGCTCTCGGCCCTGGTGGAT
  GCACAGCTGGACTACCACCGGCAGGCCGTGCAGATCCTGGACGAGCTGGCGGAGAAGCTC
  AAGCGCAGGATGCGGGAAGCTTCCTCACGCCCTAAGCGGGAGTATAAGCCCAAGCCCCGG
  GAGCCCTTTGACCTTGGAGAGCCTGAGCAGTCCAACGGGGGCTTCCCCTGCACCACAGCC
  CCCAAGATCGCAGCTTCATCGTCTTTCCGATCTTCCGACAAGCCCATCCGGACCCCTAGC
  CGGAGCATGCCGCCCCTGGACCAGCCGAGCTGCAAGGCGCTGTACGACTTCGAGCCCGAG
  AACGACGGGGAGCTGGGCTTCCATGAGGGCGACGTCATCACGCTGACCAACCAGATCGAT
  GAGAACTGGTACGAGGGCATGCTGGACGGCCAGTCGGGCTTCTTCCCGCTCAGCTACGTG
  GAGGTGCTTGTGCCCCTGCCGCAGTGA
  
  Clone variation with respect to NM_003025.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003025 unedited
  GTCGTATTTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGGCTGAGGC
  GGCGCCGGGGCGGGCGCGGAGCTGGCAAGCGGGTGGCGGAGGCGGCGCCGACGGGGACTG
  CTGAGGCGCGCAGAGGGTCGGCGGCGCCCGGGAGCCTGTCGCTGGCGCGGTCCGGGCGGG
  AGGCTCGGCGGCGGGCGGCAGCATGTCGGTGGCGGGGCTGAAGAAGCAGTTCTACAAGGC
  GAGCCAGCTGGTCAGTGAGAAGGTCGGAGGGGCCGAGGGGACCAAGCTGGATGATGACTT
  CAAAGAGATGGAGAAGAAGGTGGATGTCACCAGCAAGGCGGTGACAGAAGTGCTGGCCAG
  GACCATCGAGTACCTGCAGCCCAACCCAGCCTCGCGGGCTAAGCTGACCATGCTCAACAC
  GGTGTCCAAGATCCGGGGCCAGGTGAAGAACCCCGGCTACCCGCAGTCGGAGGGGCTTCT
  GGGCGAGTGCATGATCCGCCACGGGAAGGAGCTGGGCGGCGAGTCCAACTTTGGTGACGC
  ATTGCTGGATGCCGGCGAGTCCATGAAGCGCCTGGCAGAGGTGAAGGACTCCCTGGACAT
  CGAGGTCAAGCAGAACTTCATTGACCCCCTCCAGAACCTGTGCGAGATAGACCTGAAGGA
  GATCCAGCACCACCTGAAGATACTGGAGGGCCGCCGCCTGGACTTTGACTACNAGAAGAT
  GCGGCAGGGCAAGATCCNCGATGATGAGCTACGCCAGGCCTGGGAGAAGTTCGAGGAGTC
  CAAGGGAGTGGCAGATACCGCATGCACACCTCCTGGAACTGACTCGAGCAGGTGAGTCAG
  CTCTCGGCCCTGGTGGATGCACAGCTGGACTACANCGGCAGGCGTGCAATCCTGGACAGC
  TGCCGGAGATCTCAGCGCAGGATGCGGGAGCTTCCTACGCCTAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003025 unedited
  GTACCGCGGCACGCAATCTAGTGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTGG
  AAAAAAAAACATTTAATACTTCTGTTTACAAAATTCAAGCGTACATTTCAGTTTGCTCTG
  GACCGTGCCCAAAGCTGTGTGCTCATCTCTGCGCCCCTCATGTACTTCTGACGAGGGGGG
  TGCAGGGCAAGGCAAATCAAATCCTGGGGTCCGGAGGCTTCACTGGACCACAGGGGGAGG
  GGAATGTGAATGTGGCCTGGCCCAAATAACTCCCCATTTCATCGATTTTGCATTGGGCGA
  TAGAGGAAGCAGATGTCGGGGCTGCCTGCCTTGGTCTAAAAGAGATGGCTGGGGCCACTT
  CCCACAGGGTGAATTGGCAGCGGCTCATCAAGGGGAGCCTGGCCACCAGGGGCTGGGACA
  TGCGCTCACTGGAACCTTTGTGCTTGGCCCTCGGCAGCGCGGCTGTGGTCCCGTGTGAAG
  TGTGCTGGGGTGGGTGTGGGTGGCTTGTNTGGTGGCAACTTGTGCCATAGTGACACAAGC
  CTCCCTGGGTTGGGATGGGGGGCAGTTAAAAAGCTGAAAAGGTACTTGGCTTTCTGAAGG
  CGGGGCTTGGGAAGCAAGGCCCTGCAAGAGACCATGTTCTCTGTCCTCAAGCAGATCCCC
  AGCTGGCCTCTGTCCCCGGGCTGCAAGCTGACAGCAAGCCCGGGNAAGCGGGGGAGGCCC
  CTCTGCCCCTGCCTTGAGAAAAATGAATGCCTGTGTGAAGCCGGGGGTTCATTTGGCCCT
  GTATTAGGGCCAAGGCCCATCACCAGACCAGCTGGGAAGGGATTAAATACCAGGTCCTTA
  CTCTGAGCCACCCCTGGGGACCCCTGATCCCTCCCGGTAAGGCTTGCGGACTCAAGGGTG
  GCAAGGGGGCCCGGCCCCCCCTGGTAAGAAGGAGGTGGGGGGCGCCCCAAAGAACTTTGG
  GTTTTAAAAACCCTTTTGTTTCCCCCTTGCCACTGGGCAAAATTGGAAGCGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003025
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003025.2, NP_003016.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 1107 bp
• Locus ID: 6455
• Cytogenetics: 19p13.3
• Domains: SH3, BAR
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: 'This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).