RGS10 (NM_002925) Human Untagged Clone

CAT#: SC118312

RGS10 (untagged)-Human regulator of G-protein signaling 10 (RGS10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RGS10
• Synonyms: OTTHUMP00000020597; OTTHUMP00000069158; regulator of G-protein signaling 10; regulator of G-protein signalling 10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002925, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACACATCCACGACAGCGATGGCAGTTCCAGCAGCAGCCACCAGAGCCTCAAGAGCACAGCCAAAT
  GGGCGGCATCCCTGGAGAATCTGCTGGAAGACCCAGAAGGCGTGAAAAGATTTAGGGAATTTTTAAAAAA
  GGAATTCAGTGAAGAAAATGTTTTGTTTTGGCTAGCATGTGAAGATTTTAAGAAAATGCAAGATAAGACG
  CAGATGCAGGAAAAGGCAAAGGAGATCTACATGACCTTTCTGTCCAGCAAGGCCTCATCACAGGTCAACG
  TGGAGGGGCAGTCTCGGCTCAACGAGAAGATCCTGGAAGAACCGCACCCTCTGATGTTCCAGAAACTCCA
  GGACCAGATCTTTAATCTCATGAAGTACGACAGCTACAGCCGCTTCTTAAAGTCTGACTTGTTTTTAAAA
  CACAAGCGAACCGAGGAAGAGGAAGAAGATTTGCCTGATGCTCAAACTGCAGCTAAAAGAGCTTCCAGAA
  TTTATAACACATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002925 unedited
  GGGTAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGCGCCGTG
  AGCCGGCTGAGCAGGAAGCGGCCGCCGTCAGACATCCACGACAGCGATGGCAGTTCCAGC
  AGCAGCCACCAGAGCCTCAAGAGCACAGCCAAATGGGCGGCATCCCTGGAGAATCTGCTG
  GAAGACCCAGAAGGCGTGAAAAGATTTAGGGAATTTTTAAAAAAGGAATTCAGTGAAGAA
  AATGTTTTGTTTTGGCTAGCATGTGAAGATTTTAAGAAAATGCAAGATAAGACGCAGATG
  CAGGAAAAGGCAAAGGAGATCTACATGACCTTTCTGTCCAGCAAGGCCTCATCACAGGTC
  AACGTGGAGGGGCAGTCTCGGCTCAACGAGAAGATCCTGGAAGAACCGCACCCTCTGATG
  TTCCAGAAACTCCAGGACCAGATCTTTAATCTCATGAAGTACGACAGCTACAGCCGCTTC
  TTAAAGTCTGACTTGTTTTTAAAACACAAGCGAACCGAGGAAGAGGAAGAAGATTTGCCT
  GATGCTCAAACTGCAGCTAAAAGAGCTTCCAGAATTTATAACACATGAGCCCCCAAAAAG
  CCGGGACTGGCAGCTTTAAGAAGCAAAGGAATTTCCTCTCAGGACCGTGCCGGGTTTATC
  ATTGCTTTGTTATTTGTAAGGACTGAAATGTACAAAACCCTTCAATGGGATGTGTGTTTT
  ATTAACTGCTTCACCAGTAAATTTTGCATGATGGCTAAGCTAACATAAAAAAAAAAAAAA
  AAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCCGGTGG
  CATCCCTGTGACCCCTCCCCATGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCA
  CCAGCCTTGTCCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002925 unedited
  GGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTAGCTTAGCCAT
  CATGCAAAATTTACTGGTGAAGCAGTTAATAAAACACACATCCCATTGAAGGGTTTTGTA
  CATTTCAGTCCTTACAAATAACAAAGCAATGATAAACCCGGCACGGTCCTGAGAGGAAAT
  TCCTTTGCTTCTTAAAGCTGCCAGTCCCGGCTTTTTGGGGGCTCATGTGTTATAAATTCT
  GGAAGCTCTTTTAGCTGCAGTTTGAGCATCAGGCAAATCTTCTTCCTCTTCCTCGGTTCG
  CTTGTGTTTTAAAAACAAGTCAGACTTTAAGAAGCGGCTGTAGCTGTCGTACTTCATGAG
  ATTAAAGATCTGGTCCTGGAGTTTCTGGAACATCAGAGGGTGCGGTTCTTCCAGGATCTT
  CTCGTTGAGCCGAGACTGCCCCTCCACGTTGACCTGTGATGAGGCCTTGCTGGACAGAAA
  GGTCATGTAGATCTCCTTTGCCTTTTCCTGCATCTGCGTCTTATCTTGCATTTTCTTAAA
  ATCTTCACATGCTAGCCAAAACAAAACATTTTCTTCACTGAATTCCTTTTTTAAAAATTC
  CCTAAATCTTTTCACGCCTTCTGGGTCTTCCAGCAGATTCTCCAGGGATGCCGCCCATTT
  GGCTGTGCTCTTGAGGCTCTGGTGGCTGCTGCTGGAACTGCCATCGCTGTCGTGGATGTC
  TGACGGCGGCCGCTTNCTGCTCAGCCGGCTCCCGGCGCGCCTCGTGCCGAATTCGGGCCC
  GCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATAT
  AGACCTCCCACGTACACGCCTACGGCCCATTTGCGTCACCGGGCGGNGTTATTACGACAT
  TNTGGAAAGTTCCGTTGATTTTGGTGGCCAAACAAACTCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002925
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002925.3, NP_002916.1
• RefSeq Size: 859 bp
• RefSeq ORF: 504 bp
• Locus ID: 6001
• Cytogenetics: 10q26.11
• Domains: RGS
• Gene Summary: 'Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus compared to isoform a.