RANTES (CCL5) (NM_002985) Human Untagged Clone

CAT#: SC118321

CCL5 (untagged)-Human chemokine (C-C motif) ligand 5 (CCL5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCL5
• Synonyms: D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118321 sequence for NM_002985 edited (data generated by NextGen Sequencing)
  ATGAAGGTCTCCGCGGCAGCCCTCGCTGTCATCCTCATTGCTACTGCCCTCTGCGCTCCT
  GCATCTGCCTCCCCATATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGC
  CCACTGCCCCGTGCCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCA
  GCAGTCGTCTTTGTCACCCGAAAGAACCGCCAAGTGTGTGCCAACCCAGAGAAGAAATGG
  GTTCGGGAGTACATCAACTCTTTGGAGATGAGCTAG
  
  Clone variation with respect to NM_002985.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002985 unedited
  CCGCAGAGGGAAAGCACGTGGACCTCGCACGCCTCTTCCCCAGTACCAGAAGGTCTCCGC
  GGCAGCCCTCGCTGTCATCCTCATTGCTACTTGCCCTCTGCGCTCCTGCATCTGCCTCCC
  CTTATTCCTCGGACACCACACCCTGCTGCTTTGCCTACATTGCCCGCCCACTGCCCCGTG
  CCCACATCAAGGAGTATTTCTACACCAGTGGCAAGTGCTCCAACCCAGCAGTCGTCTTTG
  TCACCCGAAAGAACCGCCAAGTGTGTGCCAACCCAGAAAAGAAATGGGTTCGGGAGTACA
  TCAACTCTTTGGAGATGAGCTAGGATGGAGAGTCCTTGAACCTGAACTTACACAAATTTG
  CCTGTTTCTGCTTGCTCTTGTCCTAGCTTGGGAGGCTTCCCCTCACTATCCTACCCCACC
  CGCTCCTTGAAGGGCCCAGATTCTACCACACAGCAGCAGTTACAAAAACCTTCCCCAGGC
  TGGAATGGTGCTCTACGCCCTGTGTAATCCAACACTTTTGGAAGCCCAGGTGGGTTGGAT
  CACCTGANGTCAGGAGTTCGAGACCAGCCTGNCCAACATGATGAAACCCCATCTCTACTA
  AAAATACAAAAAATTAGCCGGGCGTGGTAGCGGGCGCCTGTAGTCCCGCTACTCCGGAGG
  CTGAGGCAGGAGAATGGCGTGAACCCCGGAGGCGGAACTTGCAGTGAGCCGAGATCGCGC
  CACTGCACTTCCACCTGGGCGACAGACCGAGACTCCCTCTTCAAAAAAAAAAAAAAAAAA
  AAAATCCACTCTTAAATTGCGGCCGCGGTCCTAGCTTGTTTCCTGAACAGATCCCCGGTG
  GGATCCCCTGTAACCCCTCCCCCATGCCCTTTCTTGCACCTGAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002985 unedited
  GCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTGAAACGAGTCTCGCTCTG
  TCGCCCAAGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGT
  TCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGCTACCAC
  GCCCGGCTAATTTTTTGTATTTTTAGTAAAGATGGGGTTTCATCATGTTGGCCAGGCTGG
  TCTCGAACTCCTGACCTCAGGGGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTAC
  ACGCGTGAGCCACCACGTCCAGCCTGGGGAAGGGTTTTGTAACTGCTGCTGTGTGGTAGA
  ATCTGGGCCCTTCAAGGAGCGGGTGGGGTATGATAGTGAGGGGAAGCCTCCCAAGCTAGG
  ACAAGAGCAAGCAGAAACAGGCAAATTTGTGTAAGTTCAAGTTCAAGGACTCTCCACCCT
  AACTCATCTCCAAAGAGTTGATGTACTCCCGAACCCATTTCTTCTCTGGGTTGGCACACA
  CTTGGCGGGTCTTTCGGGTGACAAAGACCACTGCTGGGTTGGAGCACTTGCCACGGGGTA
  GAAATACTCCCTGATGTGGGCCCGGGGCACTGGGCCGCCAATGTAAGCAAAACACCAAGG
  CGGGGGGTCCCAGGAATATGGGGAAGCAGATGCAGGATCCCCAAAGGCACTTTCATTGAA
  GATTACCTTCGAAGGCCGCCCAGGAAAACCTTAGGTTCCCCTGGGAAAAGGCTTCCCAAG
  ATCACCTGCCGTGCCCTCTCGCCGAATTGCCGCCCGCCCTATACGAGCCGGTTAACAAAT
  CCCGCGGGTCCCAAACGACCCCTCGTTTTTATACCTCCCCCCTCACCCCTCACCCCCTTT
  GCGTAATGGGGGGGGTTTTTCACTCTTGAAAGCCGTGTTTGGGGCAAAAATTCCCTGTAC
  GCTGGGGTGCAACGCCACCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002985
• Insert Size: 850 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002985.2, NP_002976.2
• RefSeq Size: 1237 bp
• RefSeq ORF: 276 bp
• Locus ID: 6352
• Cytogenetics: 17q12
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway
• Gene Summary: 'This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (1) represents the shorter transcript and encodes isoform 1.