PCYT2 (NM_002861) Human Untagged Clone

CAT#: SC118379

PCYT2 (untagged)-Human phosphate cytidylyltransferase 2, ethanolamine (PCYT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCYT2
• Synonyms: ET
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002861, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCCGGAACGGGCGCGGGGCTGCAGGCGGCGCAGAGCAGCCGGGCCCGGGGGGCAGGCGCGCCGTGA
  GGGTGTGGTGCGATGGCTGCTATGACATGGTGCATTACGGCCACTCCAACCAGCTGCGCCAGGCACGGGC
  CATGGGTGACTACCTCATCGTAGGCGTGCACACCGATGAGGAGATCGCCAAGCACAAGGGGCCCCCGGTG
  TTCACTCAGGAGGAGAGATACAAGATGGTGCAGGCCATCAAATGGGTGGACGAGGTGGTGCCAGCGGCTC
  CCTACGTCACTACACTAGAGACCCTGGACAAATACAACTGTGACTTCTGTGTTCACGGCAATGACATCAC
  CCTGACTGTAGATGGCCGGGACACCTATGAGGAAGTAAAGCAGGCTGGGAGGTACAGAGAATGCAAGCGC
  ACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCGCATGCTGCTGGTAACCAAAGCCCATCACAGCAGCC
  AGGAGATGTCCTCTGAGTACCGGGAGTATGCAGACAGTTTTGGCAAGTGCCCTGGTGGGCGGAACCCCTG
  GACCGGGGTATCCCAGTTCCTGCAGACATCTCAGAAGATCATCCAGTTTGCTTCTGGGAAGGAGCCCCAG
  CCAGGGGAGACAGTCATCTATGTGGCTGGTGCCTTCGACCTGTTCCACATCGGGCATGTGGACTTCCTGG
  AGAAGGTGCACAGGCTGGCAGAGAGGCCCTACATCATCGCGGGCTTACACTTTGACCAGGAGGTCAATCA
  CTACAAGGGGAAGAACTACCCCATCATGAATCTGCATGAACGGACTCTGAGCGTGCTGGCCTGCCGGTAC
  GTGTCAGAAGTGGTGATTGGAGCCCCGTACGCGGTCACAGCAGAGCTCCTAAGTCACTTCAAGGTGGACC
  TGGTGTGTCACGGCAAGACAGAAATTATCCCTGACAGGGATGGCTCCGACCCATACCAGGAGCCCAAGAG
  AAGGGGCATCTTCCGTCAGATTGACAGTGGCAGCAACCTCACCACAGACCTCATCGTCCAGCGGATCATC
  ACCAACAGGTTGGAGTATGAGGCGCGAAACCAGAAGAAGGAAGCCAAGGAGCTGGCCTTCCTGGAGGCTG
  CCAGGCAGCAGGCGGCACAGCCCCTGGGGGAGCGCGATGGTGACTTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002861 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGCTGCCAGGCT
  GTCCGCGCCGCCGCTGCGGGGCCATGATCCGGAACGGGCGCGGGGCTGCAGGCGGCGCAG
  AGCAGCCGGGCCCGGGGGGCAGGCGCGCCGTGAGGGTGTGGTGCGATGGCTGCTATGACA
  TGGTGCATTACGGCCACTCCAACCAGCTGCGCCAGGCACGGGCCATGGGTGACTACCTCA
  TCGTAGGCGTGCACACCGATGAGGAGATCGCCAAGCACAAGGGGCCCCCGGTGTTCACTC
  AGGAGGAGAGATACAAGATGGTGCAGGCCATCAAATGGGTGGACGAGGTGGTGCCAGCGG
  CTCCCTACGTCACTACACTAGAGACCCTGGACAAATACAACTGTGACTTCTGTGTTCACG
  GCAATGACATCACCCTGACTGTAGATGGCCGGGACACCTATGAGGAAGTAAAGCAGGCTG
  GGAGGTACAGAGAATGCAAGCGCACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCGCA
  TGCTGCTGGTAACCAAAGCCCATCACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGAGT
  ATGCAGACAGTTTTGGCAAGTGCCCTGGTGGGCGGAACCCCTGGACCGGNGTATCCCAGT
  TCCTGCAGACATCTCAGAAGATCATCCAGTTTGCTTCTGGGAAGGAGCCCCAGCCAGGGG
  AGACAGTCATCTATGTGGGCTGGNTGCCTTTCGACCTGTNCACATCGGGCATGTGGACTT
  CCTGGAGAANGTGCACAGCTGGCANNAGAGCCCTACTCNATCGCGGNNCTACACTTTGAC
  CAGGTCACTGCCTTCTTGCTCCCTGGCCCTGGCAAAANTTGATGGCNCCTCCCAGGGCCC
  TANACCNTGCTTGTAAGNGCCCAGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002861 unedited
  GCCTTCCCGACAGGNCTGTTTAGCCCCGCGCAGCACTGCCACCAGGCTTTATTCGGTTGT
  ACCAAANAGATTTCCGTCAGCCATGTACAAACAGAACAGAGTCCATAAGGAGAGTGGCGA
  AGCTGCCGGGAAGGAAGGCAAGCGTGTTTTCCTGGAGCCAGGCGCTGGGTGGCAGGACAC
  CGTGTTGGGGGGTGCCCAGTGGGAGGTGGTGTGGTCTACACCCAGAGAACTAAAGGGGTG
  ACCGCAGTCACTGCTCCCTAAAGCCCTCTGCTCTGCACCTCCCGCCACAGCTGGGTGGGC
  AGTCAGTGTGGCCTCTGTCCAGGTGGTGGGGGCATCCGGGGACCAGGTGGGGGCGCACGC
  AGGAGCGGTGCGTTTCAGGCTGCAGGTGCCGTCTGCCCGTCTCACTTCCGGCCTCTCCAC
  TGTGCTGGACACCCTCTCTGAGCAGCTTTGCTGGAAAGAGCGGAGAGCCTGCTGCAAACC
  AGGCACCTTGTAGGCAGGCAAGGAGGCAGAGTCCTCACCAGCCCTTCCAGAGCCAGGCCA
  GTTATAGAGGGCGGTCCTGTAGAGTCCTATGTCTAAACGCATATTGCGCACCATGCAGAC
  CAGGGGGAGGGCCGGCTATTGCCTTCTGCCTGTTACAAGTCACCCATCGTGCTCCCCCAG
  GGCTGTGCCCGCCTGTTTGCCTCGCAGCCTTCATGAGGCCAGCTCCTTGGTTTCCTCCTT
  CTGGTTTCGCGCCTCATACTCCAACCTGTTGGTGACGATCCGTTTGACGATGATGTCTGT
  GGTGAGGGTGCCTGCACTGTCATCTGATGGAATATGCCCTTCTCTTGGCTTCTTGTATGG
  TGCCGGAGTCTCCCTGTCCGGGAAATTTCCTTTTGGCTGTTACCACCCCGCCCACTTTCA
  ATGATTTAGGACCTCTCCGGGACCCGCGCCGGGTTTCATCCCTCTTTTTGCCCTTCCGGC
  GGTCACACGCTACATTCTCCTGCCATCTTTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002861
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002861.1, NP_002852.1
• RefSeq Size: 1856 bp
• RefSeq ORF: 1170 bp
• Locus ID: 5833
• Cytogenetics: 17q25.3
• Domains: CTP_transf_2
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.