PSMD10 (NM_002814) Human Untagged Clone

CAT#: SC118385

PSMD10 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (PSMD10), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD10
• Synonyms: dJ889N15.2; p28; p28(GANK)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118385 sequence for NM_002814 edited (data generated by NextGen Sequencing)
  ATGGAGGGGTGTGTGTCTAACCTAATGGTCTGCAACCTGGCCTACAGCGGGAAGCTGGAA
  GAGTTGAAGGAGAGTATTCTGGCCGATAAATCCCTGGCTACTAGAACTGACCAGGACAGC
  AGAACTGCATTGCACTGGGCATGCTCAGCTGGACATACAGAAATTGTTGAATTTTTGTTG
  CAACTTGGAGTGCCAGTGAATGATAAAGACGATGCAGGTTGGTCTCCTCTTCATATTGCG
  GCTTCTGCTGGCCGGGATGAGATTGTAAAAGCCCTTCTGGGAAAAGGTGCTCAAGTGAAT
  GCTGTCAATCAAAATGGCTGTACTCCCTTACATTATGCAGCTTCGAAAAACAGGCATGAG
  ATCGCTGTCATGTTACTGGAAGGCGGGGCTAATCCAGATGCTAAGGACCATTATGAGGCT
  ACAGCAATGCACCGGGCAGCAGCCAAGGGTAACTTGAAGATGATTCATATCCTTCTGTAC
  TACAAAGCATCCACAAACATCCAAGACACTGAGGGTAACACTCCTCTACACTTAGCCTGT
  GATGAGGAGAGAGTGGAAGAAGCAAAACTGCTGGTGTCCCAAGGAGCAAGTATTTACATT
  GAGAATAAAGAAGAAAAGACACCCCTGCAAGTGGCCAAAGGTGGCCTGGGTTTAATACTC
  AAGAGAATGGTGGAAGGTTAA
  
  Clone variation with respect to NM_002814.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002814 unedited
  CAGCCATATTTGTNAATACGCACTCACTATAGGGNNCGGCCGCGAAATTCGGCACGAGGG
  GACAGCGAAATGGAGGGGTGTGTGTCTAACCTAATGGTCTGCAACCTGGCCTACAGCGGG
  AAGCTGGAAGAGTTGAAGGAGAGTATTCTGGCCGATAAATCCCTGGCTACTAGAACTGAC
  CAGGACAGCAGAACTGCATTGCACTGGGCATGCTCAGCTGGACATACAGAAATTGTTGAA
  TTTTTGTTGCAACTTGGAGTGCCAGTGAATGATAAAGACGATGCAGGTTGGTCTCCTCTT
  CATATTGCGGCTTCTGCTGGCCGGGATGAGATTGTAAAAGCCCTTCTGGGAAAAGGTGCT
  CAAGTGAATGCTGTCAATCAAAATGGCTGTACTCCCTTACATTATGCAGCTTCGAAAAAC
  AGGCATGAGATCGCTGTCATGTTACTGGAAGGCGGGGCTAATCCAGATGCTAAGGACCAT
  TATGAGGCTACAGCAATGCACCGGGCAGCAGCCAAGGGTAACTTGAAGATGATTCATATC
  CTTCTGTACTACAAAGCATCCACAAACATCCAAGACACTGAGGGTAACACTCCTCTACAC
  TTAGCCTGTGATGAGGAGAGAGTGGAAGAAGCAAAACTGCTGGTGTCCCAAGGAGCAAGT
  ATTTACATTGAGAAATAAGAAGACCAGACACCTCTGCAAGTGGCCCAAGGTGGCCCTGGT
  TTAATACTCAAGAGAATGGGGAACGTTAAACAGCTTGGATTTATTCTTACTTTGTATGTT
  GTGTTGTTGGCCCCAGTGTCCTACAACTAATGAATTTGTGCACCAGACATCATCTATGAA
  TGATGAAGTTTCTTCACCTTAAAGTCTAAAAACATGTTGACTCTTGTCCTGCTGACTACA
  TTGTGAAGCTACACTTGGTTTCAGCCTCGAAAACTGTGAGATTGTCTTACGGGTCGAAAA
  TCTCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002814 unedited
  GCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCTTTCAACATTTTATTTTTT
  AGTTTCTGAAATACAAATCAAAATACATTTTTGTTACATGTCATTTTAACAAGCTTGACA
  TTCTTTTCAATACAGGATGACACAAAATAGGTACTTTAAAACTTCCTTTTAAGAAAACCA
  TACATTTTATTTCAGAGTAAACAACCCATCAAAAACAAATACATAGGTATATGGGCTATT
  GTTTTTCAGTATTTTAGAGCACACCATCCAACTTAAAATGTGGTCCACTAAAATATGTAA
  ATATCCCTCCTATCACAAAAATAAATTAAACCTCATGGTTAAAAAAAACAGAAACGTTTG
  GCGTAGGCCTGAGTGAGCTTGAAAAACAAGTTAGGATGTTTTAACTGTATGGAGGAACAT
  TACAGAATCCACATAGAAACATCTGGCAGAACAACTAGCTTGTTGCTATTGGAGCTGTTC
  TGTTTCAGAGAGGGATATAAGGTACATGGGAGGGTGCTGAAGACTCACAACAGCCACAGA
  ATTAGTTACTCCAAATTAACCAGAATTCAATATAGAAGAATATACGACACCAGTAGAACA
  ATCTCAACAGTTATTCGATGCCTGGAAAACAAGCTGTAAGCTTCGAACAAGTAACTCAGC
  AGGAACAAGAGTCAACATGTTTATAAAGACTTTGAAGGTGAGAAAAACTCATCATTCATA
  GATGATGTCTTGGGCACCAATACATTAGTTGGTAGGACACTGGGGACACCACCCCACCTA
  AAAGTAAGATAATTCCAGCTGTTTAACTTTCCCCATTCTCTTGAGGATTAAACCCAGCCA
  CCTTTGCCCACTGCAGGGNTGTCTTTTCTTCTTATTTCCAAGGAAAACCTGCTCCTTGGG
  ACCAAAAGGTTTGTCTCTCCATCCTTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002814
• Insert Size: 1600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002814.2, NP_002805.1
• RefSeq Size: 1546 bp
• RefSeq ORF: 681 bp
• Locus ID: 5716
• Cytogenetics: Xq22.3
• Domains: ANK
• Gene Summary: 'This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform.