PRSS8 (NM_002773) Human Untagged Clone

CAT#: SC118401

PRSS8 (untagged)-Human protease, serine, 8 (PRSS8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRSS8
• Synonyms: CAP1; PROSTASIN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118401 sequence for NM_002773 edited (data generated by NextGen Sequencing)
  ATGGCCCAGAAGGGGGTCCTGGGGCCTGGGCAGCTGGGGGCTGTGGCCATTCTGCTCTAT
  CTTGGATTACTCCGGTCGGGGACAGGAGCGGAAGGGGCAGAAGCTCCCTGCGGTGTGGCC
  CCCCAAGCACGCATCACAGGTGGCAGCAGTGCAGTCGCCGGTCAGTGGCCCTGGCAGGTC
  AGCATCACCTATGAAGGCGTCCATGTGTGTGGTGGCTCTCTCGTGTCTGAGCAGTGGGTG
  CTGTCAGCTGCTCACTGCTTCCCCAGCGAGCACCACAAGGAAGCCTATGAGGTCAAGCTG
  GGGGCCCACCAGCTAGACTCCTACTCCGAGGACGCCAAGGTCAGCACCCTGAAGGACATC
  ATCCCCCACCCCAGCTACCTCCAGGAGGGCTCCCAGGGCGACATTGCACTCCTCCAACTC
  AGCAGACCCATCACCTTCTCCCGCTACATCCGGCCCATCTGCCTCCCTGCAGCCAACGCC
  TCCTTCCCCAACGGCCTCCACTGCACTGTCACTGGCTGGGGTCATGTGGCCCCCTCAGTG
  AGCCTCCTGACGCCCAAGCCACTGCAGCAACTCGAGGTGCCTCTGATCAGTCGTGAGACG
  TGTAACTGCCTGTACAACATCGACGCCAAGCCTGAGGAGCCGCACTTTGTCCAAGAGGAC
  ATGGTGTGTGCTGGCTATGTGGAGGGGGGCAAGGACGCCTGCCAGGGTGACTCTGGGGGC
  CCACTCTCCTGCCCTGTGGAGGGTCTCTGGTACCTGACGGGCATTGTGAGCTGGGGAGAT
  GCCTGTGGGGCCCGCAACAGGCCTGGTGTGTACACTCTGGCCTCCAGCTATGCCTCCTGG
  ATCCAAAGCAAGGTGACAGAACTCCAGCCTCGTGTGGTGCCCCAAACCCAGGAGTCCCAG
  CCCGACAGCAACCTCTGTGGCAGCCACCTGGCCTTCAGCTCTGCCCCAGCCCAGGGCTTG
  CTGAGGCCCATCCTTTTCCTGCCTCTGGGCCTGGCTCTGGGCCTCCTCTCCCCATGGCTC
  AGCGAGCACTGA
  
  Clone variation with respect to NM_002773.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002773 unedited
  AGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGGTGACTCG
  TCCACACTGCTCGCTTCGGATACTCCAGGCGTCTCCCGTTGCGGCCGCTCCCTGCCTTAG
  AGGCCAGCCTTGGACACTTGCTGCCCCTTTCCAGCCCGGATTCTGGGATCCTTCCCTCTG
  AGCCAACATCTGGGTCCTGCCTTCGACACCACCCCAAGGCTTCCTACCTTGCGTGCCTGG
  AGTCTGCCCCAGGGGCCCTTGTCCTGGGCCATGGCCCAGAAGGGGGTCCTGGGGCCTGGG
  CAGCTGGGGGCTGTGGCCATTCTGCTCTATCTTGGATTACTCCGGTCGGGGACAGGAGCG
  GAAGGGGCAGAAGCTCCCTGCGGTGTGGCCCCCCAAGCACGCATCACAGGTGGCAGCAGT
  GCAGTCGCCGGTCAGTGGCCCTGGCAGGTCAGCATCACCTATGAAGGCGTCCATGTGTGT
  GGTGGCTCTCTCGTGTCTGAGCAGTGGGTGCTGTCAGCTGCTCACTGCTTCCCCAGCGAG
  CACCACAAGGAAGCCTATGAGGTCAAGCTGGGGGCCCACCAGCTAGACTCCTACTCCGAG
  GACGCCAAGGTCAGCACCCTGAAGGACATCATCCCCCACCCCAGCTACCTCCAGGAGGGC
  TCCCAGGGCGACATTGCACTCCTCCAACTCAGCAGACCCATCACCTTCTCCCGCTACATC
  CGGCCCATCTGCCTCCCTGCAGCCAACGCCTNCTTCCCCACGGCCTCCACTGCACTGTCA
  CTGGCTGGGGTCATGTGGCCCCCTCAGTGAGCCTCCTGACGCCCAAGCCACTGCAGCAAC
  TCGAGGTGCCTCTGATCAGTCGTGAGACGTGTAACTTGCTGTACACATCGACGCCAACCC
  TGAGACCGCACTTTGCCCAGAGACATGGTGTGTGCTGGCTATGTGAGGGGGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002773 unedited
  ATCTTGNCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTGCTCAAACA
  TTTTAATCATTTCTGCCCTGTTACTCCCACCCCAAATCCAAGCGCCAGCCCAGTTCCGGT
  GGGGGCTCAGTCCTCCGGAGTCCAGGAGTCAGGGCTCGGGGGCGCTCAGCGGCCAGTGGG
  CAAGATTGGGGCCTTTCCTGTCCTCGAAGCTGCACAAAGGTGGCCCCAGCCCAGAACACA
  GGGAGAGGGCAGAGAGATGTGCTCATCAGTCTGGGCAGGCGGGCCGGGAGCAGTCTTCCA
  GAAACAGGTGGGAGCCAGGGCTCATTTTCATAGCCAAGGGTCCCAGGAGCTCCCCAGGAG
  CTCGGCCAATGGGCTGGTCCATGGTGGGTGAGGGGCCAGAGGCTCTGGCCATTGCTACAG
  GCAGTAAAACTCCTGACTCTCAGTGATGGTCCCAAAAAGCACACCCAGAAGAATGGGCTC
  AAAGATCAAGATGGGGCCCCAGCCTCCCGCAGAGTCCTGAAGGAAGGAGTGGCTCAAGTC
  AGGCCCTGGGTCCAAGGCCATCAGGGAAGGACCANGCTCCTGTCCTTGAGTGTGATGCAT
  CCATCCTGGAAGTAGGGCCAGCTCAGTGCTCGCTGAGCCCTGGGAAGAGAGGCCCAAAGC
  CGGCCCCAGAGCCAGAAAAGATGGGCCTCAGCAAGCCCTGGGCTGGGGCANAACTGAAGC
  CACGTGGCTGCACANAAGTTGCTGTCGGGCTGGGACTCCTGGTTTGGGGCACCCACCAGC
  CTGGAGTTTTGTCACCTTGCTTTGGATCCACGAGGCCTACCTGGAAGGCCCAGGGGTCCC
  CCAGGCCTGTTGGGGGCCCCCAGGCTTTTCCCAGTTAAAATGCCGGCAGGGACCAAAGAC
  CTCCCCAGGCCGGGAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002773
• Insert Size: 1770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002773.2, NP_002764.1
• RefSeq Size: 1938 bp
• RefSeq ORF: 1032 bp
• Locus ID: 5652
• Cytogenetics: 16p11.2
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation. [provided by RefSeq, Feb 2016]'