PRSS8 (NM_002773) Human Untagged Clone

CAT#: SC320203

PRSS8 (untagged)-Human protease, serine, 8 (PRSS8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRSS8
• Synonyms: CAP1; PROSTASIN
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002773.3 CCGTTGCGGCCGCTCCCTGCCTTAGAGGCCAGCCTTGGACACTTGCTGCCCCTTTCCAGC
  CCGGATTCTGGGATCCTTCCCTCTGAGCCAACATCTGGGTCCTGCCTTCGACACCACCCC
  AAGGCTTCCTACCTTGCGTGCCTGGAGTCTGCCCCAGGGGCCCTTGTCCTGGGCCATGGC
  CCAGAAGGGGGTCCTGGGGCCTGGGCAGCTGGGGGCTGTGGCCATTCTGCTCTATCTTGG
  ATTACTCCGGTCGGGGACAGGAGCGGAAGGGGCAGAAGCTCCCTGCGGTGTGGCCCCCCA
  AGCACGCATCACAGGTGGCAGCAGTGCAGTCGCCGGTCAGTGGCCCTGGCAGGTCAGCAT
  CACCTATGAAGGCGTCCATGTGTGTGGTGGCTCTCTCGTGTCTGAGCAGTGGGTGCTGTC
  AGCTGCTCACTGCTTCCCCAGCGAGCACCACAAGGAAGCCTATGAGGTCAAGCTGGGGGC
  CCACCAGCTAGACTCCTACTCCGAGGACGCCAAGGTCAGCACCCTGAAGGACATCATCCC
  CCACCCCAGCTACCTCCAGGAGGGCTCCCAGGGCGACATTGCACTCCTCCAACTCAGCAG
  ACCCATCACCTTCTCCCGCTACATCCGGCCCATCTGCCTCCCTGCAGCCAACGCCTCCTT
  CCCCAACGGCCTCCACTGCACTGTCACTGGCTGGGGTCATGTGGCCCCCTCAGTGAGCCT
  CCTGACGCCCAAGCCACTGCAGCAACTCGAGGTGCCTCTGATCAGTCGTGAGACGTGTAA
  CTGCCTGTACAACATCGACGCCAAGCCTGAGGAGCCGCACTTTGTCCAAGAGGACATGGT
  GTGTGCTGGCTATGTGGAGGGGGGCAAGGACGCCTGCCAGGGTGACTCTGGGGGCCCACT
  CTCCTGCCCTGTGGAGGGTCTCTGGTACCTGACGGGCATTGTGAGCTGGGGAGATGCCTG
  TGGGGCCCGCAACAGGCCTGGTGTGTACACTCTGGCCTCCAGCTATGCCTCCTGGATCCA
  AAGCAAGGTGACAGAACTCCAGCCTCGTGTGGTGCCCCAAACCCAGGAGTCCCAGCCCGA
  CAGCAACCTCTGTGGCAGCCACCTGGCCTTCAGCTCTGCCCCAGCCCAGGGCTTGCTGAG
  GCCCATCCTTTTCCTGCCTCTGGGCCTGGCTCTGGGCCTCCTCTCCCCATGGCTCAGCGA
  GCACTGAGCTGGCCCTACTTCCAGGATGGATGCATCACACTCAAGGACAGGAGCCTGGTC
  CTTCCCTGATGGCCTTTGGACCCAGGGCCTGACTTGAGCCACTCCTTCCTTCAGGACTCT
  GCGGGAGGCTGGGGCCCCATCTTGATCTTTGAGCCCATTCTTCTGGGTGTGCTTTTTGGG
  ACCATCACTGAGAGTCAGGAGTTTTACTGCCTGTAGCAATGGCCAGAGCCTCTGGCCCCT
  CACCCACCATGGACCAGCCCATTGGCCGAGCTCCTGGGGAGCTCCTGGGACCCTTGGCTA
  TGAAAATGAGCCCTGGCTCCCACCTGTTTCTGGAAGACTGCTCCCGGCCCGCCTGCCCAG
  ACTGATGAGCACATCTCTCTGCCCTCTCCCTGTGTTCTGGGCTGGGGCCACCTTTGTGCA
  GCTTCGAGGACAGGAAAGGCCCCAATCTTGCCCACTGGCCGCTGAGCGCCCCCGAGCCCT
  GACTCCTGGACTCCGGAGGACTGAGCCCCCACCGGAACTGGGCTGGCGCTTGGATCTGGG
  GTGGGAGTAACAGGGCAGAAATGATTAAAATGTTTGAGCACAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002773
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002773.3, NP_002764.1
• RefSeq Size: 1940 bp
• RefSeq ORF: 1032 bp
• Locus ID: 5652
• Cytogenetics: 16p11.2
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation. [provided by RefSeq, Feb 2016]'