Proteasome 20S alpha 5 (PSMA5) (NM_002790) Human Untagged Clone

CAT#: SC118413

PSMA5 (untagged)-Human proteasome (prosome, macropain) subunit, alpha type, 5 (PSMA5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMA5
• Synonyms: PSC5; ZETA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118413 sequence for NM_002790 edited (data generated by NextGen Sequencing)
  ATGTTTCTTACCCGGTCTGAGTACGACAGGGGCGTGAATACTTTTTCTCCCGAAGGAAGA
  TTATTTCAAGTGGAATATGCCATTGAGGCTATCAAGCTTGGTTCTACAGCCATTGGGATC
  CAGACATCAGAGGGTGTGTGCCTAGCTGTGGAGAAGAGAATTACTTCCCCACTGATGGAG
  CCCAGCAGCATTGAGAAAATTGTAGAGATTGATGCTCACATAGGTTGTGCCATGAGTGGG
  CTAATTGCTGATGCTAAGACTTTAATTGATAAAGCCAGAGTGGAGACACAGAACCACTGG
  TTCACCTACAATGAGACAATGACAGTGGAGAGTGTGACCCAAGCTGTGTCCAATCTGGCT
  TTGCAGTTTGGAGAAGAAGATGCAGATCCAGGTGCCATGTCTCGTCCCTTTGGAGTAGCA
  TTATTATTTGGAGGAGTTGATGAGAAAGGACCCCAGCTGTTTCATATGGACCCATCTGGG
  ACCTTTGTACAGTGTGATGCTCGAGCAATTGGCTCTGCTTCAGAGGGTGCCCAGAGCTCC
  TTGCAAGAAGTTTACCACAAGTCTATGACTTTGAAAGAAGCCATCAAGTCTTCACTCATC
  ATCCTCAAACAAGTAATGGAGGAGAAGCTGAATGCAACAAACATTGAGCTAGCCACAGTG
  CAGCCTGGCCAGAATTTCCACATGTTCACAAAGGAAGAACTTGAAGAGGTTATCAAGGAC
  ATTTAA
  
  Clone variation with respect to NM_002790.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002790 unedited
  GGTACTATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGTTGGGTGCC
  GGTGGAGTCGTGTTGGTCCTCAGAATCCCCGCGTAGCCGCTGCCTCCTCCTACCCTCGCC
  ATGTTTCTTACCCGGTCTGAGTACGACAGGGGCGTGAATACTTTTTCTCCCGAAGGAAGA
  TTATTTCAAGTGGAATATGCCATTGAGGCTATCAAGCTTGGTTCTACAGCCATTGGGATC
  CAGACATCAGAGGGTGTGTGCCTAGCTGTGGAGAAGAGAATTACTTCCCCACTGATGGAG
  CCCAGCAGCATTGAGAAAATTGTAGAGATTGATGCTCACATAGGTTGTGCCATGAGTGGG
  CTAATTGCTGATGCTAAGACTTTAATTGATAAAGCCAGAGTGGAGACACAGAACCACTGG
  TTCACCTACAATGAGACAATGACAGTGGAGAGTGTGACCCAAGCTGTGTCCAATCTGGCT
  TTGCAGTTTGGAGAAGAAGATGCAGATCCAGGTGCCATGTCTCGTCCCTTTGGAGTAGCA
  TTATTATTTGGAGGAGTTGATGAGAAAGGACCCCAGCTGTTTCATATGGACCCATCTGGG
  ACCTTTGTACAGTGTGATGCTCGAGCAATTGGCTCTGCTTCAGAGGGTGCCCAGAGCTNC
  TTGNCAGAGTTTACCCACAGTCTATGACTTTGAAAGAAGCCATCAAGTCTTCACTCATCA
  TCCTCAAACAAGTAATGGGAGAGAAAGCTGATGCAACANACATTGAGCTAGCCACAGTGC
  AGCCTGGCCAGAATTTCCACATGTTCACAAAGGAAGAACTTGAAGAGTTATCCAAGACAT
  TTAAGGAATCCTGATCCTCAAAACTCTCTGGGACAATTNCAGTNCTAATATGGCCCTAAA
  TTTTATTTCAGCTCCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002790 unedited
  CGGCCACGGCCAATCCTNAGAAANTNCGAAGNTCTTTCTTCTTCTTCTTCTTCTTCTTAA
  TCTTCTAAAAAAATTTCTNCTCTTTATCTTCCAGAAAACATGGCCTCTTCATGTCACAGC
  ACATCACTTTAAAAAATGCACATACAATGGAGATTTTCCAAGGAACAGGAGCTGGAAATA
  AAATTTAAGGACATTATTAGAACTGAAATTGTCCCAGAGAAGTTCTGAGGATCAGGATTC
  CTTAAATGTCCTTGATAACCTCTTCAAGTTCTTCCTTTGTGAACATGTGGAAATTCTGGC
  CAGGCTGCACTGTGGCTAGCTCAATGTTTGTTGCATTCAGCTTCTCCTCCATTACTTGTT
  TGAGGATGATGAGTGAAGACTTGATGGCTTCTTTCAAAGTCATAGACTTGTGGTAAACTT
  CTTGCAAGGAGCTCTGGGCACCCTCTGAAGCAGAGCCAATTGCTCGAGCATCACACTGTA
  CAAAGGTCCCAGATGGGTCCATATGAAACAGCTGGGGTCCTTTCTCATCAACTCCTCCAA
  ATAATAATGCTACTCCAAAGGGACGAGACATGGCACCTGGATCTGCATCTTCTTCTCCAA
  ACTGCAAAGCCAGATTGGACACAGCTTGGGTCACACTCTCCACTGTCATTGTCTCATTGC
  AAGTGAACCCATGGTTCTGTGCCTCCACTCTGGCTTCACAATTAAAGTCTTAACCTTAGC
  AATTACCCCACTCATGGCACAACCTATGGGACCACAATCCCTTACATTTTCTCAATGCCG
  CTGGGCCTCCATCAAGGCGGAAATAATCTTCTTCTCCACAGCTTGGCCCACACCCTCTGA
  AGCCCGGACCCAAAGGCTGTAAAAACCACCTGGTAACCCCAAGGGCTATTCCACTGGAAT
  AATCTTCCTTGGGAGAAAAATTCCACCCCCCTGTCTCCCCAACGGGTAAAAACATGGCAG
  GCCCGGAGGAGGCATGGTCTCCCGGATTTTAGACCAATCTATCCCGGCACCCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002790
• Insert Size: 880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002790.2, NP_002781.2
• RefSeq Size: 1023 bp
• RefSeq ORF: 726 bp
• Locus ID: 5686
• Cytogenetics: 1p13.3
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]'
  Transcript Variant: This variant (1) is the predominant transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.