MAPK11 (NM_002751) Human Untagged Clone

CAT#: SC118466

MAPK11 (untagged)-Human mitogen-activated protein kinase 11 (MAPK11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK11
• Synonyms: p38-2; P38B; p38Beta; P38BETA2; PRKM11; SAPK2; SAPK2B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002751 edited
  GAATTCGCACGAGGCTCGGGCGCGGGCGCGGGGCGCGGGGCTGGGCCCGGGCGGAGCGGC
  GGCTGCTCCGGACATGTCGGGCCCTCGCGCCGGCTTCTACCGGCAGGAGCTGAACAAGAC
  CGTGTGGGAGGTGCCGCAGCGGCTGCAGGGGCTGCGCCCGGTGGGCTCCGGCGCCTACGG
  CTCCGTCTGTTCGGCCTACGACGCCCGGCTGCGCCAGAAGGTGGCGGTGAAGAAGCTGTC
  GCGCCCCTTCCAGTCGCTGATCCACGCGCGCAGAACGTACCGGGAGCTGCGGCTGCTCAA
  GCACCTGAAGCACGAGAACGTCATCGGGCTTCTGGACGTCTTCACGCCGGCCACGTCCAT
  CGAGGACTTCAGCGAAGTGTACTTGGTGACCACCCTGATGGGCGCCGACCTGAACAACAT
  CGTCAAGTGCCAGGCGCTGAGCGACGAGCACGTTCAATTCCTGGTTTACCAGCTGCTGCG
  CGGGCTGAAGTACATCCACTCGGCCGGGATCATCCACCGGGACCTGAAGCCCAGCAACGT
  GGCTGTGAACGAGGACTGTGAGCTCAGGATCCTGGATTTCGGGCTGGCGCGCCAGGCGGA
  CGAGGAGATGACCGGCTATGTGGCCACGCGCTGGTACCGGGCACCTGAGATCATGCTCAA
  CTGGATGCATTACAACCAAACAGTGGATATCTGGTCCGTGGGCTGCATCATGGCTGAGCT
  GCTCCAGGGCAAGGCCCTCTTCCCGGGAAGCGACTACATTGACCAGCTGAAGCGCATCAT
  GGAAGTGGTGGGCACACCCAGCCCTGAGGTTCTGGCAAAAATCTCCTCAGAACACGTGAG
  TTGGTGCCCGCCAGCCAGCAGCTCCCTCTCCTGGCTGAGCCCCCAGGCTCTCTGGGGGAG
  AGGGTGGTGTGTTAATGCGGGGACCATATGGGGAACATGGGATGTGGTGGGATAGAGCCT
  AGGGCAGGTGCCCAATCCCTGCAGGGGAAACAGGAGAAGGGGGAAGACATTCCTGACACC
  AGGGACAGTGAGTGCAAAGCCATGACCCCAGCAAGCAGTGCCTGGCCAGGGCAGTGGGGG
  TGGGCTGGGGGCCCAGCAAGCAGTGCCTGGCCAGGGCAGTGGGGGTGGGCTGGGGGACCC
  CAGCAAGCAGTGCCTGGCCAGGGCAGTGGGGGTGGGCTGGGTGGAGGGAGTGTACTTGGA
  CCCAGCTCCTGCAGGCATTAGGGGCCAACTGAGGAGCTAGAACCACCAGATCCTATATGG
  AGGGGGTCACTGGTCTGCCTGTGCCCTTGTCCTGGCCTGTTGGAGTGAATGAGTCTGGGG
  GCTTGCCAAGAGAAGCCCCAGTTTGGGGCCAGGGAGGCAGAGAGTGAGCAGCCTCCCCAG
  GTGTTCTTGTGTGCCCCCAGGCCCGGACATATATCCAGTCCCTGCCCCCCATGCCCCAGA
  AGGACCTGAGCAGCATCTTCCGTGGAGCCAACCCCCTGGCCATAGACCTCCTTGGAAGGA
  TGCTGGTGCTGGACAGTGACCAGAGGGTCAGTGCAGCTGAGGCACTGGCCCACGCCTACT
  TCAGCCAGTACCACGACCCCGAGGATGAGCCAGAGGCCGAGCCATATGATGAGAGCGTTG
  AGGCCAAGGAGCGCACGCTGGAGGAGTGGAAGGAGCTCACTTACCAGGAAGTCCTCAGCT
  TCAAGCCCCCAGAGCCACCGAAGCCACCTGGCAGCCTGGAGATTGAGCAGTGAGGTGCTG
  CCCAGCAGCCCCTGAGAGCCTGTGGAGGGGCTTGGGCCTGCACCCTTCCACAGCTGGCCT
  GGTTTCCTCGAGAGGCACCTCCCACACTCCTATGGTCACAGACTTCTGGCCTAGGACCCC
  TCGCCTTCAGGAGAATCTACACGCATGTATGCATGCACAAACATGTGTGTACATGTGCTT
  GCCATGTGTAGGAGTCTGGGCACAAGTGTCCCTGGGCCTACCTTGGTCCTCCTGTCCTCT
  TCTGGCTACTGCACTCTCCACTGGGACCTGACTGTGGGGTCCTAGATGCCAAAGGGGTTC
  CCCTGCGGAGTTCCCCTGTCTGTCCCAGGCCGACCCAAGGGAGTGTCAGCCTTGGGCTCT
  CTTCTGT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002751 unedited
  AGACCAAGGACCAACAACTTTCNNCCCCCCCGTTCAGATTTGTATACGATCATATAGGCG
  GCCGCGNAATTCGCACGAGGCTCGGGCGCGGGCGCGGGGCGCGGGGCTGGGCCCGGGCGG
  AGCGGCGGCTGCTCCGGACATGTCGGGCCCTCGCGCCGGCTTCTACCGGCAGGAGCTGAA
  CAAGACCGTGTGGGAGGTGCCGCAGCGGCTGCAGGGGCTGCGCCCGGTGGGCTCCGGCGC
  CTACGGCTCCGTCTGTTCGGCCTACGACGCCCGGCTGCGCCAGAAGGTGGCGGTGAAGAA
  GCTGTCGCGCCCCTTCCAGTCGCTGATCCACGCGCGCAGAACGTACCGGGAGCTGCGGCT
  GCTCAAGCACCTGAAGCACGAGAACGTCATCGGGCTTCTGGACGTCTTCACGCCGGCCAC
  GTCCATCGAGGACTTCAGCGAAGTGTACTTGGTGACCACCCTGATGGGCGCCGACCTGAA
  CAACATCGTCAAGTGCCAGGCGCTGAGCGACGAGCACGTTCAATTCCTGGTTTACCAGCT
  GCTGCGCGGGCTGAAGTACATCCACTCGGCCGGGATCATCCACCGGGACCTGAAGCCCAG
  CAACGTGGCTGTGAACGAGGACTGTGAGCTCAGGATCCTGGATTTCGGGCTGGCGCGCCA
  GGCGGACCAGGAGACGACCGGCTATGTGGCCACGCGCTGGTACCGGGCCCCTGACACATG
  CCCCACTGGATGCATTACCACCAACCAGTGGATACTCCCGGCCGCGGGCCTGCACTATGC
  GCTGACCTGCTCCAGGGCAAGGGCCCCTCTTCCCCGGGAAGCGACCTACATTGACCCAGC
  TAAAACCCATAATGTGAACGGGTGGGCCACACACACACCTTGGGCTTTTCGGAACAAATT
  TCCTACAACTCACTTAATTCCCGCGCCGAACAACCCCATATCTACCTCTCTCTGGCCGCA
  CCCCCCACGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002751 unedited
  GCGCTCGAGGACCCCCTTTTACGTGCATCCCTGGTGCAGGCACTTGNCACCCAGGCTTCC
  CTGGGCCCCTCGAGGCAAGAGGTCACCCCCTTTCCAGGCAGAGCCACACCCCCAACTCAG
  CTCTGGGCAGGGTCCCGTCTCCGAGGTTACTGGATCAGCTCCATCCTGGGACAAGGAAAG
  AGGACTGACCCACAGACCAGAGCACCTCAGATCTCCGGCTGCAGGGCCACAACGGTGGGC
  AGAGGTAGAGAGCGGGCCAGGGTGCAGGGCAGAAGTGTCCGAGTCCAAGTCCACATCCAG
  GTGTGCTGCCTGCCCTAGCGTCCTCAAGGCCAAACCACCCACCCACCCACCCCCAGCCTC
  AGGAAGCTGCTGTGGGTCCCACATCCAAGGAGAAAGCCCCTGCCCATGCTCATGTGGTTG
  CCCTTTGGCCCACTGGAGCCGCCAGACTCAAACACCCCTCAACGCATCAGGCCCCGTCCG
  ATAGGGAACGTAACAGCCCACCCCCCCTATCTTCACCTCCTTGCCAGACCTGGCCGTTCG
  CCTTGGGCCCCCACACACTTCCACGCCCCTCATTACATGTCCCCTCGTCCTTCCATCCCC
  GTGGCGTGCACCCCCTCTTTCCACCTGTTCTCCCATCTCTCGCCGGTCGCATCTCTTCAT
  CCCCAGACATCCCCGACTCATCTTGTCACTTTGGTGGCTCCCCCCTCCCCCACTCCCCCT
  GTCACTCTGCTTCGCCTCCCCTCTACTCAACACTCCCATGTTTCCCCCGCCGTTCTTTTT
  CCCCCCTCTCTTCCCCTTCTTTCCCCCTCCTCCTATTCCTTCCCCTCACCTCGATTCTTT
  TTCCCCCTCCCCGCCCTTTTTGCCCATTTATCCCCCGTACCCAACACACACACCCTTTCT
  CCCCCCCTTCTCTTCGTTTCCCTGTTTCCACTTCGCTGCGTCCGCTTTATCCCCCCTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002751
• Insert Size: 1120 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002751.3, NP_002742.3
• RefSeq Size: 1310 bp
• RefSeq ORF: 1095 bp
• Locus ID: 5600
• Cytogenetics: 22q13.33
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway
• Gene Summary: 'This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the protein.