PAFAH1B2 (NM_002572) Human Untagged Clone

CAT#: SC118559

PAFAH1B2 (untagged)-Human platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa) (PAFAH1B2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAFAH1B2
• Synonyms: HEL-S-303
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002572, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCAAGGAGACTCAAACCCAGCAGCTATTCCGCATGCAGCAGAAGATATTCAAGGAGATGACCGAT
  GGATGTCTCAGCACAACAGATTTGTTTTGGACTGTAAAGACAAAGAGCCTGATGTACTGTTCGTGGGAGA
  CTCCATGGTGCAGTTAATGCAGCAATATGAGATATGGCGAGAGCTTTTTTCCCCACTTCATGCACTGAAT
  TTTGGAATTGGGGGAGATACAACAAGACATGTTTTGTGGAGACTAAAGAATGGAGAACTGGAGAATATTA
  AGCCTAAGGTCATTGTTGTCTGGGTAGGAACAAATAACCACGAAAATACAGCAGAAGAAGTAGCAGGTGG
  GATCGAGGCCATTGTACAACTTATCAACACAAGGCAGCCACAGGCCAAAATCATTGTATTGGGTTTGTTA
  CCTCGAGGTGAGAAACCCAATCCTTTGAGGCAAAAGAACGCCAAGGTGAACCAACTCCTCAAGGTTTCGC
  TGCCGAAGCTTGCCAACGTGCAGCTCCTGGATACCGACGGGGGTTTTGTGCACTCGGACGGTGCCATCTC
  CTGCCACGACATGTTTGATTTTCTGCATCTGACAGGAGGGGGCTATGCAAAGATCTGCAAACCCCTGCAT
  GAACTGATCATGCAGTTGTTGGAGGAAACACCTGAGGAGAAACAAACCACCATTGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002572 unedited
  GGTGCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCACGGACCC
  TCTACTTCAGTGTAGAATGAGCCAAGGAGACTCAAACCCAGCAGCTATTCCGCATGCAGC
  AGAAGATATTCAAGGAGATGACCGATGGATGTCTCAGCACAACAGATTTGTTTTGGACTG
  TAAAGACAAAGAGCCTGATGTACTGTTCGTGGGAGACTCCATGGTGCAGTTAATGCAGCA
  ATATGAGATATGGCGAGAGCTTTTTTCCCCACTTCATGCACTGAATTTTGGAATTGGGGG
  AGATACAACAAGACATGTTTTGTGGAGACTAAAGAATGGAGAACTGGAGAATATTAAGCC
  TAAGGTCATTGTTGTCTGGGTAGGAACAAATAACCACGAAAATACAGCAGAAGAAGTAGC
  AGGTGGGATCGAGGCCATTGTACAACTTATCAACACAAGGCAGCCACAGGCCAAAATCAT
  TGTATTGGGTTTGTTACCTCGAGGTGAGAAACCCAATCCTTTGAGGCAAAAGAACGCCAA
  GGTGAACCAACTCCTCAAGGTTTCGCTGCCGAAGCTTGCCAACGTGCAGCTCCTGGATAC
  CGACGGGGGTTTTGTGCACTCGGACGGTGCCATCTCCTGCCACGACATGTTTGATTTTCT
  GCATCTGACAGGAGGGGGCTATGCAAAGATCTGCAAACCCCTGCATGAACTGATCATGCA
  GTTGTTGGAGGAAACACCTGAGGAGAAACAACCACCATTGCCTGACTGGCTCTTATCAGT
  GTTAATAGCATCTCAGCTTCCTCAGATCAGTTCTATCACTGGCACTACAGAATCCTTCTC
  TTTCTTAGGCCACTTGCATTGTAAATGTTCCTGAATGTCATATCTAGTGTTTGAAGGGGA
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002572
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002572.1, NP_002563.1
• RefSeq Size: 1195 bp
• RefSeq ORF: 690 bp
• Locus ID: 5049
• Cytogenetics: 11q23.3
• Domains: Lipase_GDSL
• Protein Pathways: Ether lipid metabolism, Metabolic pathways
• Gene Summary: 'Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.