NFYA (NM_002505) Human Untagged Clone

CAT#: SC118595

NFYA (untagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFYA
• Synonyms: CBF-A; CBF-B; HAP2; NF-YA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118595 sequence for NM_002505 edited (data generated by NextGen Sequencing)
  ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA
  CAGATTCAGCAGCAGCAGCAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAG
  GTGGCATCCGCCTCAGGCCAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTA
  ATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAG
  GCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGT
  TTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTG
  CAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCT
  GTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCA
  CAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTC
  CAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGCAGGA
  GCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGACTGTC
  ACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATGGTT
  CCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTT
  GAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAA
  GCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCAT
  GAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTTCTCT
  CCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGATGAAGAAGCAATG
  ACACAGATCATCCGAGTGTCCTAA
  
  Clone variation with respect to NM_002505.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002505 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCG
  GCACGAGGCGGCGGCAGCGGCGGCTGGAGCCTCTGATTGGGTTTCGGAGTCCGGTACTGG
  AGCCAATCAGCGCGGGCAGCGAACCGGGGGAGCGAGGCACGGAGTGTACCTCACAGCCTT
  CTAGGATCTCCAGAGTGGACAGGAATCTCACTTGGAGGGACCATGGAGCAGTATACAGCA
  AACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGCAGCAGCAG
  CAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAGGTGGCATCCGCCTCAGGC
  CAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGA
  GGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAGGCTGTCCCTGGTGGACAA
  GGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGTTTGCAGCAAATACAGTTG
  GTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGCAGGGCCAGCAG
  GGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGACT
  CAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAG
  ACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAGTTACAGTCCCTG
  GTTCAGGCATGATCACTATCCAGCAGCCAGTTTGGGCAGAGCCCAGATTGTNAAACAGGA
  GCCATACCACACACCAGCAGTGGCAAGGGACTGTACTGTGCACTACAGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002505
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002505.3, NP_002496.1
• RefSeq Size: 2834 bp
• RefSeq ORF: 1044 bp
• Locus ID: 4800
• Cytogenetics: 6p21.1
• Domains: CBF
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation
• Gene Summary: 'The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) includes alternate exon B, resulting in the long isoform, isoform 1.