M6PR (NM_002355) Human Untagged Clone

CAT#: SC118693

M6PR (untagged)-Human mannose-6-phosphate receptor (cation dependent) (M6PR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: M6PR
• Synonyms: CD-M6PR; CD-MPR; MPR-46; MPR 46; MPR46; SMPR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118693 sequence for NM_002355 edited (data generated by NextGen Sequencing)
  ATGTTCCCTTTCTACAGCTGCTGGAGGACTGGACTGCTACTACTACTCCTGGCTGTGGCA
  GTGAGAGAATCCTGGCAGACAGAAGAAAAAACTTGCGACTTGGTAGGAGAAAAGGGTAAA
  GAGTCAGAGAAAGAGTTGGCTCTAGTGAAGAGGCTGAAACCACTGTTTAATAAAAGCTTT
  GAGAGCACTGTGGGCCAGGGTTCAGACACATACATCTACATCTTCAGGGTGTGCCGGGAA
  GCTGGCAACCACACTTCTGGGGCAGGCCTGGTGCAAATCAACAAAAGTAATGGGAAGGAG
  ACAGTGGTAGGGAGACTCAACGAGACTCACATCTTCAACGGAAGTAATTGGATCATGCTG
  ATCTATAAAGGGGGTGATGAATATGACAACCACTGTGGCAAGGAGCAGCGTCGTGCAGTG
  GTGATGATCTCCTGCAATCGACACACCCTAGCGGACAATTTTAACCCTGTGTCTGAGGAG
  CGTGGCAAAGTCCAAGATTGTTTCTACCTCTTTGAGATGGATAGCAGCCTGGCCTGTTCA
  CCAGAGATCTCCCACCTCAGTGTGGGTTCCATCTTACTTGTCACGTTTGCATCACTGGTT
  GCTGTTTATGTTGTTGGGGGGTTCCTATACCAGCGACTGGTAGTGGGAGCCAAAGGAATG
  GAGCAGTTTCCCCACTTAGCCTTCTGGCAGGATCTTGGCAACCTGGTAGCAGATGGCTGT
  GACTTTGTCTGCCGTTCTAAACCTCGAAATGTGTCTGCAGCATATCGTGGTGTGGGGGAT
  GACCAGCTGGGGGAGGAGTCAGAAGAAAGGGATGACCATTTATTACCAATGTAG
  
  Clone variation with respect to NM_002355.3
  754 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002355 unedited
  GTGTTCAGCCATATTTGTNAATACGCACTCACTATAGGGCGGCCGCGAATTCGCCGAGGG
  CCTCTGAACTAGCTCTGGGACCGGGGTCTGCGGCCGGCCCCTAGCTGGCCCCGTCTCCCA
  TCCCCAGAAGGGTATTCACTGGGGATTCTGAGCTTTGGCTACTCCAGTTTCCCACGACAC
  GATGTTCCCTTTCTACAGCTGCTGGAGGACTGGACTGCTACTACTACTCCTGGCTGTGGC
  AGTGAGAGAATCCTGGCAGACAGAAGAAAAAACTTGCGACTTGGTAGGAGAAAAGGGTAA
  AGAGTCAGAGAAAGAGTTGGCTCTAGTGAAGAGGCTGAAACCACTGTTTAATAAAAGCTT
  TGAGAGCACTGTGGGCCAGGGTTCAGACACATACATCTACATCTTCAGGGTGTGCCGGGA
  AGCTGGCAACCACACTTCTGGGGCAGGCCTGGTGCAAATCAACAAAAGTAATGGGAAGGA
  GACAGTGGTAGGGAGACTCAACGAGACTCACATCTTCAACGGAAGTAATTGGATCATGCT
  GATCTATAAAGGGGGTGATGAATATGACAACCACTGTGGCAAGGAGCAGCGTCGTGCAGT
  GGTGATGATCTCCTGCAATCGACACACCCTAGCGGACAATTTTAACCCTGTGTCTGAGGA
  GCGTGGCAAAGTCCAAGATTGTTTCTACCTCTTTGAGATGGATAGCAGCCTGGCCTGTTC
  ACCAGAGATCTCCCACCTCAGTGTGGGTTCCATCTTACTTGTNCACGTTGCATCACTGGT
  TGCTGTTTAATGTGTTGGNGGGTTCCTATCCCGCGACTGTTAGTGGGAGCCAAAGGATGG
  AGCAGTTCCCCACTTACCCTCTGGCAGATCCTGGCACCCTCTAGCAGAGGCTGTGACTTG
  TCTGCGTTCTAACCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002355 unedited
  CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTGGGGGGTTTTTAAACATTAAAAAATTTTGTACCAAAGGGGAAGGGT
  TTTTTTTTTTATTTTAAAACACCAAGGGGGGGGGGAGGGATGCCAACCAATAACAAAAAA
  AAAGCTTTTGGAACATTATTTTCCCTGTTTAAAAAAAAAAAAAACACTCCCATAATATTG
  AAAAGTCCAAAAAAGAAAAAATTTCATTTTTTTTTCCTAAGGGTTAAAAAAAGGCCCCTG
  CCTGTTGAATCCCTCCCTTTTTTGGGGCCAGGGGAGCCCTGTTACTTTTTAAGGGCCCAC
  GGGGTTACTATTAAAAAAAGAACAATCCAAGTTTCTGGGCCCCTGGCCCAAACAGGAAAA
  TGGGAGCCTCTGAGGATTAAAAACCTTTCCCCACAAAAATGGGGGGCAAAAAAAACTTTC
  CTTAACCAACAAAAAGGACAGGTGCCCTATTATTTCACACCCACCGCACAAGAACGCAAA
  ACCCCCCATGGCCCACTCTCTGGCTTCCGTCCCTTCTTAAAAAACTGAAAAAAAGGGCAC
  AACCCACTCCCTGTGGAAAAAAAGGCCACATTGTTTAAAAAAACCGGGGGGGGAAACACC
  AATTAAAAAAACCCTTCCCACTTTTTTCTACACATGGTGGAAATAAATTTTGGGTCCCCC
  CTCCCCCACGGGCAAACGGCCTTCCCCTTTTTTGGGAACCCCTGGGTGAAAAGAGTAACC
  ATTTTTCCTTTCCCAACACACTGGATTCCTTGCAAAGATTTTTTGCTGCTTGTGACACAA
  AAAACACCAATACTTAATCCCATTTCAATTTTCAAAGTCCCCCAATTTCCAACAAAGATG
  TGAGGCGCCCGCATAATCCCCGCAATTGGGAACACCTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002355
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002355.2, NP_002346.1
• RefSeq Size: 2454 bp
• RefSeq ORF: 834 bp
• Locus ID: 4074
• Cytogenetics: 12p13.31
• Domains: Man-6-P_recep
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: 'This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).