Plastin L (LCP1) (NM_002298) Human Untagged Clone

CAT#: SC118739

LCP1 (untagged)-Human lymphocyte cytosolic protein 1 (L-plastin) (LCP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCP1
• Synonyms: CP64; HEL-S-37; L-PLASTIN; LC64P; LPL; PLS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118739 sequence for NM_002298 edited (data generated by NextGen Sequencing)
  ATGGCCAGAGGATCAGTGTCCGATGAGGAAATGATGGAGCTCAGAGAAGCTTTTGCCAAA
  GTTGATACTGATGGCAATGGATACATCAGCTTCAATGAGTTGAATGACTTGTTCAAGGCT
  GCTTGCTTGCCTTTGCCTGGGTATAGAGTACGAGAAATTACAGAAAACCTGATGGCTACA
  GGTGATCTGGACCAAGATGGAAGGATCAGCTTTGATGAGTTTATCAAGATTTTCCATGGC
  CTAAAAAGCACAGATGTTGCCAAGACCTTTAGAAAAGCAATCAATAAGAAGGAAGGGATT
  TGTGCAATCGGTGGTACTTCAGAGCAGTCTAGCGTTGGCACCCAACACTCCTATTCAGAG
  GAAGAAAAGTATGCCTTTGTCAACTGGATAAACAAAGCCCTGGAAAATGATCCTGATTGT
  CGGCATGTCATCCCAATGAACCCAAACACGAATGATCTCTTTAATGCTGTTGGAGATGGC
  ATTGTCCTTTGTAAAATGATCAACCTGTCAGTGCCAGACACAATTGATGAAAGAACAATC
  AACAAAAAGAAGCTAACCCCTTTCACCATTCAGGAAAATCTGAACTTGGCTCTGAACTCT
  GCCTCAGCCATCGGGTGCCATGTGGTCAACATAGGGGCTGAGGACCTGAAGGAGGGGAAG
  CCTTATCTGGTCCTGGGACTTCTGTGGCAAGTCATCAAGATTGGGTTGTTTGCTGACATT
  GAACTCAGCAGAAATGAAGCTCTGATTGCTCTTTTGAGAGAAGGTGAGAGCCTGGAGGAT
  TTGATGAAACTCTCCCCTGAAGAGCTCTTGCTGAGGTGGGCTAATTACCACCTGGAAAAT
  GCAGGCTGCAACAAAATTGGCAACTTCAGTACTGACATCAAGGACTCAAAAGCTTATTAC
  CACCTGCTTGAGCAGGTGGCTCCAAAAGGAGATGAAGAAGGTGTTCCTGCTGTTGTTATT
  GACATGTCAGGACTGCGGGAGAAGGATGACATCCAGAGGGCAGAATGCATGCTGCAGCAG
  GCGGAGAGGCTGGGCTGCCGGCAGTTTGTCACAGCCACAGATGTTGTCCGAGGGAACCCC
  AAGTTGAACTTGGCTTTTATTGCCAACCTCTTTAACAGATACCCTGCCCTGCACAAACCA
  GAGAACCAGGACATTGACTGGGGGGCTCTTGAAGGTGAGACGAGAGAAGAGCGGACATTT
  AGGAACTGGATGAACTCCCTGGGTGTTAACCCTCGAGTCAATCATTTGTACAGTGACTTA
  TCAGATGCCCTGGTCATCTTCCAGCTCTATGAAAAGATCAAAGTTCCTGTTGACTGGAAC
  AGAGTAAACAAACCGCCATACCCCAAACTGGGAGGCAATATGAAGAAGCTTGAGAATTGT
  AACTACGCGGTAGAATTGGGGAAGAATCAAGCGAAGTTCTCCCTGGTTGGCATCGGTGGA
  CAAGATCTCAATGAAGGAAACCGCACTCTCACACTGGCCTTGATTTGGCAGCTAATGAGA
  AGGTATACACTGAATATCCTCGAAGAAATTGGTGGTGGCCAGAAGGTCAATGATGACATT
  ATTGTCAACTGGGTGAATGAAACATTGAGGGAAGCAGAGAAAAGTTCATCCATCTCTAGT
  TTCAAGGACCCTAAGATTAGTACAAGTCTGCCTGTTCTGGACCTCATCGATGCCATCCAA
  CCAGGTTCCATTAACTATGACCTTCTGAAGACAGAAAATCTGAATGATGATGAGAAACTC
  AACAATGCAAAATATGCCATCTCTATGGCCCGAAAAATTGGAGCAAGAGTGTATGCCCTG
  CCAGAAGACCTGGTTGAAGTGAACCCCAAAATGGTCATGACCGTGTTTGCCTGCCTCATG
  GGGAAAGGAATGAAGAGGGTGTGA
  
  Clone variation with respect to NM_002298.4
  1597 a=>g;1632 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002298 unedited
  TTCACTATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCCACC
  TTTTTCCCTGGCTCTTTCACTAAAAAGAAAAATTGTTGATCTTTGCTGTAAGTAGTCACT
  TCCTGCCTTGTGACCACACACCCAGGCTTGACAAAGCTGTTCTGCAGATCAGAAAGAAGG
  GGTTCCTGGTCATACACCAGTACTACCAAGGACAGCTTTTTTCCTGCAAGATCTGTTACC
  TAAAGCAATAAAAAATGGCCAGAGGATCAGTGTCCGATGAGGAAATGATGGAGCTCAGAG
  AAGCTTTTGCCAAAGTTGATACTGATGGCAATGGATACATCAGCTTCAATGAGTTGAATG
  ACTTGTTCAAGGCTGCTTGCTTGCCTTTGCCTGGGTATAGAGTACGAGAAATTACAGAAA
  ACCTGATGGCTACAGGTGATCTGGACCAAGATGGAAGGATCAGCTTTGATGAGTTTATCA
  AGATTTTCCATGGCCTAAAAAGCACAGATGTTGCCAAGACCTTTAGAAAAGCAATCAATA
  AGAAGGAAGGGATTTGTGCAATCGGTGGTACTTCAGAGCAGTCTAGCGTTGGCACCCAAC
  ACTCCTATTCAGAGGAAGAAAAGTATGCCTTTGTCAACTGGATAAACAAAGCCCTGNAAA
  ATGATCCTGATTGTCGGCATGTCATCCCAATGAACCCAAACACGAATGATCTCTTTAATG
  CTGTTGGAGATGGCATTGTCCTTTGTAAAAGATCACCTGTCAGTGCCAAAACAATTGATG
  AAAGACAATCACAAAAAGGAGCTACCCCTTTCACCATTCAGGAAATCTGAACTTGGCTCT
  GAATCTGCCTCAACCATCCGGTGCCATGTGGCCACATAGGGGCTGAGGACCTGAAGGAGG
  GAAGCCTTACTCGTCCCTGGACTTTGTGGCAGTCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002298 unedited
  GCGGCCGCATTTAGNATCGAGTTTTTTTTTTTTTTTTTTATAGGGAACATTTTATTTGGA
  AAGATTTTGAGCATCATTCTCTTTTCATTTTTTATTAAGGCAGTAACATTCTTTCTTCCT
  GGAGGCGGAGGGGGGAAATCATCTGAGGCTACAGTAAGTTCAATCTGAAGTCAAAACCAA
  CCAATTTGTATTCAGAAGCAGCAAAAATACTGGTTATGGGCTGGGAGGAGCCACATGTTG
  GCTTTCTGAGTCCACTGCCAAGGAACAGATCCAGAGCAATGCATTTCAATTGCTTTGAAA
  AATGGCTAAGTTCCATTACATACCACCACTAAAGAAGATACCTCATTGATGGAGCTTTTC
  ACCATGATAGAGGGCGTCTTGCATTGGTTCACAATGGCCTGTTGCTTTTTAGCCTTCAGT
  TGGTTTAGGGGGAAATTGCTCAACCTATGAGACCTGCTCAAATTCATACTGTTCTGGTTT
  TGCTTTGGAATTCTTTGTCCAGTAAATTAGCTCTTCATAACGCAGTTCCACCATTTCTTA
  TCCAGCATAGATTCATTCTGCCCTTGACAGCTGGCTAGGTGTGTGTGTATGTGAGTAGGG
  GTGCTATTGATTGGCACATATTGTCCCCCCTGGAGTTTAGGGGTGCACAAAGCTTTATAG
  TACCAAAAAAGTAAACTTGATAATATGGCCCGCAACCATCAATATGCTAAGCCTTAAAAC
  AAAAGCAGGCAGGATTGCCCCCAACTGTTCCAGGCTTAACCCTACCCACAGCACCTAATC
  CATGGTGAGGCTAATGCCCGGAAAGCAGAACCCTAAGGACGCTAACTTAGCTCCTTCTGC
  CCCCAAGGCTTGGGTCCAATAACCCTCTTGGACCCCCTTCCATACGCGCCAGGTTTTAGG
  GCCAACCCCTACAACCTCCACCGCTCAACCCACTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002298
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002298.2, NP_002289.1
• RefSeq Size: 3723 bp
• RefSeq ORF: 1884 bp
• Locus ID: 3936
• Cytogenetics: 13q14.13
• Domains: CH, EFh
• Gene Summary: 'Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]'