HMG1 (HMGB1) (NM_002128) Human Untagged Clone

CAT#: SC118802

HMGB1 (untagged)-Human high mobility group box 1 (HMGB1)


  "NM_002128" in other vectors (7)

Reconstitution Protocol

USD 540.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HMGB1
Synonyms HMG-1; HMG1; HMG3; SBP-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002128, the custom clone sequence may differ by one or more nucleotides


ATGGGCAAAGGAGATCCTAAGAAGCCGAGAGGCAAAATGTCATCATATGCATTTTTTGTGCAAACTTGTC
GGGAGGAGCATAAGAAGAAGCACCCAGATGCTTCAGTCAACTTCTCAGAGTTTTCTAAGAAGTGCTCAGA
GAGGTGGAAGACCATGTCTGCTAAAGAGAAAGGAAAATTTGAAGATATGGCAAAAGCGGACAAGGCCCGT
TATGAAAGAGAAATGAAAACCTATATCCCTCCCAAAGGGGAGACAAAAAAGAAGTTCAAGGATCCCAATG
CACCCAAGAGGCCTCCTTCGGCCTTCTTCCTCTTCTGCTCTGAGTATCGCCCAAAAATCAAAGGAGAACA
TCCTGGCCTGTCCATTGGTGATGTTGCGAAGAAACTGGGAGAGATGTGGAATAACACTGCTGCAGATGAC
AAGCAGCCTTATGAAAAGAAGGCTGCGAAGCTGAAGGAAAAATACGAAAAGGATATTGCTGCATATCGAG
CTAAAGGAAAGCCTGATGCAGCAAAAAAGGGAGTTGTCAAGGCTGAAAAAAGCAAGAAAAAGAAGGAAGA
GGAGGAAGATGAGGAAGATGAAGAGGATGAGGAGGAGGAGGAAGATGAAGAAGATGAAGATGAAGAAGAA
GATGATGATGATGAATAA


>OriGene 5' read for NM_002128 unedited
ACTCACTATAGGCGGCCGCGAATTCGCACGAGGAGAGTGAGGAGGCTGCGTCTGGCTCCC
GCTCTCACAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGTGAGAG
CCGGACGGGCACTGGGCGACTCTGTGCCTCGCTGAGGAAAAATAACTAAACATGGGCAAA
GGAGATCCTAAGAAGCCGAGAGGCAAAATGTCATCATATGCATTTTTTGTGCAAACTTGT
CGGGAGGAGCATAAGAAGAAGCACCCAGATGCTTCAGTCAACTTCTCAGAGTTTTCTAAG
AAGTGCTCAGAGAGGTGGAAGACCATGTCTGCTAAAGAGAAAGGAAAATTTGAAGATATG
GCAAAAGCGGACAAGGCCCGTTATGAAAGAGAAATGAAAACCTATATCCCTCCCAAAGGG
GAGACAAAAAAGAAGTTCAAGGATCCCAATGCACCCAAGAGGCCTCCTTCGGCCTTCTTC
CTCTTCTGCTCTGAGTATCGCCCAAAAATAAAGGAGAACATCCTGGCCTGTCCATTGGTG
ATGTTGCGAAGAAACTGGGAGAGATGTGGAATAACACTGCTGCAGATGACAAGCAGCCTT
ATGAAAAGAAGGCTGCGAAGCTGAAGGAANAATACAAAAGGATATTGCTGCATATCGAGC
TAAAGAAAGCCTGATGCAGCAAANAGGGGAGTGTCAAGGGCTGAAAAAGNCAGAAAAGAA
GGAAGAAGANGAAGATGANGAANATGAANAGGATGANNGAGAGNANGAAGATGAANAANA
TGAAGATGAAGAAAAGATGATGATGATGAAATAAGNTGGTTCTAAGCGCAGTTTTTTTTT
TCTTGGCTATAAGCATTTTACCCCCCCTGTACACAACTNACTTCTTTTAAGAAAAAATTG
AATGNANGGCTGGGTAANAATTGTTTTAAACTGNACAGGCCTTTTTTTGGATAGGTAACA
CCTACCCAAGTGGGTTAAAAAACCTGGCCCGGGGGGATTTCAAAGCACCTACCTGCTGAC
GGNGGGGGGA
>OriGene 3' read for NM_002128 unedited
NNNTTAGCTCTGNNACCGCGGCCGCATTCTAGGATCGAGNTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTAACTAAAAAAAAAAATGGATTTACTTAAAAGCATTCA
AAATGGCAACAAAACAGCTGCAACTTTTTTTTTTTTGCAATTACAGAGGGGTATTCAGTT
AACAAAACAACAATTATTTCGTATAAGCTGCATCAAAGACAACTGAAGATGAAAAAACTA
CCATCCCCATATATAACTAATTTGGGCTGGGCCCCAACAAAAACCTGCTTTAAATTTCCA
TGCCAATTTACAACCCCCATACTGTACCAGGCAAGGTTAGGGGCTATTGAAAATACCACC
AGGACAGGGCTATCTAAAGACACATTCGGGAGGGGGTTAACTATACAAAAAAAGACACTG
GACAGTTTAAAAACAAATTTTACACAGCCTTACATTTAAATTTTTTTCTTTAAAAGGAGT
GAGTTGGGCACAGGGGGGTTAAATGCTTTATAGACAAAAAAAAAAAAACTGCGCTAGAAC
CAACTTATTCATCATCATCATCTTCTTCTTAATCTTCATATTCTTAATCTTCCTCCTCCT
CCCCATCCCCTTTATCTTCCTCATCTTCCTCCTCTTCCTTTCTTTTCTTGCTTTTTNCAG
CCTTGACAACTCCCTTTTTTGCTGGATAAGGCCTTTCCTTTAACCCGAAAAGCCGAAATA
ACCCTTTTGGAATTTTTCCTTTAGCTTCCCAACCTCTTTTTTATAAGGCGGCTTGCATTC
GGCAGAGGGTTTTTCCCAATCTTCCCCGGTTTTTCCCACCACCACAAGGGCCAGCCCAGG
AGGTCCCTTTTGTTTTTGGGCCAACCCCAAAACCAAAGAGAAAAAGCGCCCAAGAAGCCC
TCTGGGGGC
Restriction Sites NotI-NotI     
ACCN NM_002128
Insert Size 1320 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002128.3, NP_002119.1
RefSeq Size 3428 bp
RefSeq ORF 648 bp
Locus ID 3146
Cytogenetics 13q12.3
Domains HMG
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways Base excision repair
Gene Summary 'This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

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