HMG1 (HMGB1) (NM_002128) Human Untagged Clone

CAT#: SC324540

HMGB1 (untagged)-Human high mobility group box 1 (HMGB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMGB1
• Synonyms: HMG-1; HMG1; HMG3; SBP-1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002128.4 CAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCAAGTGAGAGCCGGACGGGCACTGGG
  CGACTCTGTGCCTCGCTGAGGAAAAATAACTAAACATGGGCAAAGGAGATCCTAAGAAGC
  CGAGAGGCAAAATGTCATCATATGCATTTTTTGTGCAAACTTGTCGGGAGGAGCATAAGA
  AGAAGCACCCAGATGCTTCAGTCAACTTCTCAGAGTTTTCTAAGAAGTGCTCAGAGAGGT
  GGAAGACCATGTCTGCTAAAGAGAAAGGAAAATTTGAAGATATGGCAAAAGCGGACAAGG
  CCCGTTATGAAAGAGAAATGAAAACCTATATCCCTCCCAAAGGGGAGACAAAAAAGAAGT
  TCAAGGATCCCAATGCACCCAAGAGGCCTCCTTCGGCCTTCTTCCTCTTCTGCTCTGAGT
  ATCGCCCAAAAATCAAAGGAGAACATCCTGGCCTGTCCATTGGTGATGTTGCGAAGAAAC
  TGGGAGAGATGTGGAATAACACTGCTGCAGATGACAAGCAGCCTTATGAAAAGAAGGCTG
  CGAAGCTGAAGGAAAAATACGAAAAGGATATTGCTGCATATCGAGCTAAAGGAAAGCCTG
  ATGCAGCAAAAAAGGGAGTTGTCAAGGCTGAAAAAAGCAAGAAAAAGAAGGAAGAGGAGG
  AAGATGAGGAAGATGAAGAGGATGAGGAGGAGGAGGAAGATGAAGAAGATGAAGATGAAG
  AAGAAGATGATGATGATGAATAAGTTGGTTCTAGCGCAGTTTTTTTTTTTTCTTGTCTAT
  AAAGCATTTAACCCCCCTGTACACAACTCACTCCTTTTAAAGAAAAAAATTGAAATGTAA
  GGCTGTGTAAGATTTGTTTTTAAACTGTACAGTGTCTTTTTTTGTATAGTTAACACACTA
  CCGAATGTGTCTTTAGATAGCCCTGTCCTGGTGGTATTTTCAATAGCCACTAACCTTGCC
  TGGTACAGTATGGGGGTTGTAAATTGGCATGGAAATTTAAAGCAGGTTCTTGTTGGTGCA
  CAGCACAAATTAGTTATATATGGGGATGGTAGTTTTTTCATCTTCAGTTGTCTCTGATGC
  AGCTTATACGAAATAATTGTTGTTCTGTTAACTGAATACCACTCTGTAATTGCAAAAAAA
  AAAAAGTTGCAGCTGTTTTGTTGACATTCTGAATGCTTCTAAGTAAATACAATTTTTTTT
  ATTAGTATTGTTGTCCTTTTCATAGGTCTGAAATTTTTCTTCTTGAGGGGAAGCTAGTCT
  TTTGCTTTTGCCCATTTTGAATCACATGAATTATTACAGTGTCTATCCTTTCATATAGTT
  AGCTAATAAAAAGCTTTTGTCTACACACCCTGCATATCATAATGGGGGTAAAGTTAAGTT
  GAGATAGTTTTCATCCATAACTGAACATCCAAAATCTTGATCAGTTAAGAAATTTCACAT
  AGCCCACTTACATTTACAAACTGAAGAGTAATCAATCTACTCAAAGCATGGGATTATTAG
  AATCAAACATTTTGAAAGTCTGTCCTTGAAGGACTAATAGAAAAGTATGTTCTAACCTTT
  ACATGAGGACTCTATTCTTTAACTCCCATTACCATGTAATGGCAGTTATATTTTGCAGTT
  CCCACATTAAAGAAGACCTGAGAATGTATCCCCAAAAGCGTGAGCTTAAAATACAAGACT
  GCCATATTAAATTTTTTGTTGACATTAGTCTCAGTGAAGACTATGAAAATGCTGGCTATA
  GATGTCTTTTCCCATTTATCTAAATATGGACTGCTCAGGAAACGAGACTTTCATTACAAG
  TATTTTTAATTAATTGGGCCAGCTTTTCAAACAAAGATGCCACATTCAAAATAGGGTATA
  TTTTCCTATATTACGGTTTGCCCCTTTATAAATCCAAGTAGATAGGAAGAAAGAAGACAA
  ACTTTGCATCTCAGTATGAATTATTCAATTTATTTGAATGATTTTTCTTTACAAAACAAA
  CTCATTCATTAGTCATGTTTATCTGCTTAGGAGTTTAGGGAACAATTTGGCAATTTTGTG
  GTTTTCGAGATTATCGTTTTCTTAAAGTGCCAGTATTTTAAAATAGCGTTCTTGTAATTT
  TACACGCTTTTGTGATGGAGTGCTGTTTTGTTATATAATTTAGACTTGGATTCTTTCCAT
  TTGCATTTGTTTATGTAATTTCAGGAGGAATACTGAACATCTGAGTCCTGGATGATACTA
  ATAAACTAATAATTGCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002128
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002128.4, NP_002119.1
• RefSeq Size: 3428 bp
• RefSeq ORF: 648 bp
• Locus ID: 3146
• Cytogenetics: 13q12.3
• Domains: HMG
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair
• Gene Summary: 'This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.