Granulin (GRN) (NM_002087) Human Untagged Clone

CAT#: SC118822

GRN (untagged)-Human granulin (GRN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRN
• Synonyms: CLN11; GEP; GP88; PCDGF; PEPI; PGRN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118822 sequence for NM_002087 edited (data generated by NextGen Sequencing)
  ATGTGGACCCTGGTGAGCTGGGTGGCCTTAACAGCAGGGCTGGTGGCTGGAACGCGGTGC
  CCAGATGGTCAGTTCTGCCCTGTGGCCTGCTGCCTGGACCCCGGAGGAGCCAGCTACAGC
  TGCTGCCGTCCCCTTCTGGACAAATGGCCCACAACACTGAGCAGGCATCTGGGTGGCCCC
  TGCCAGGTTGATGCCCACTGCTCTGCCGGCCACTCCTGCATCTTTACCGTCTCAGGGACT
  TCCAGTTGCTGCCCCTTCCCAGAGGCCGTGGCATGCGGGGATGGCCATCACTGCTGCCCA
  CGGGGCTTCCACTGCAGTGCAGACGGGCGATCCTGCTTCCAAAGATCAGGTAACAACTCC
  GTGGGTGCCATCCAGTGCCCTGATAGTCAGTTCGAATGCCCGGACTTCTCCACGTGCTGT
  GTTATGGTCGATGGCTCCTGGGGGTGCTGCCCCATGCCCCAGGCTTCCTGCTGTGAAGAC
  AGGGTGCACTGCTGTCCGCACGGTGCCTTCTGCGACCTGGTTCACACCCGCTGCATCACA
  CCCACGGGCACCCACCCCCTGGCAAAGAAGCTCCCTGCCCAGAGGACTAACAGGGCAGTG
  GCCTTGTCCAGCTCGGTCATGTGTCCGGACGCACGGTCCCGGTGCCCTGATGGTTCTACC
  TGCTGTGAGCTGCCCAGTGGGAAGTATGGCTGCTGCCCAATGCCCAACGCCACCTGCTGC
  TCCGATCACCTGCACTGCTGCCCCCAAGACACTGTGTGTGACCTGATCCAGAGTAAGTGC
  CTCTCCAAGGAGAACGCTACCACGGACCTCCTCACTAAGCTGCCTGCGCACACAGTGGGG
  GATGTGAAATGTGACATGGAGGTGAGCTGCCCAGATGGCTATACCTGCTGCCGTCTACAG
  TCGGGGGCCTGGGGCTGCTGCCCTTTTACCCAGGCTGTGTGCTGTGAGGACCACATACAC
  TGCTGTCCCGCGGGGTTTACGTGTGACACGCAGAAGGGTACCTGTGAACAGGGGCCCCAC
  CAGGTGCCCTGGATGGAGAAGGCCCCAGCTCACCTCAGCCTGCCAGACCCACAAGCCTTG
  AAGAGAGATGTCCCCTGTGATAATGTCAGCAGCTGTCCCTCCTCCGATACCTGCTGCCAA
  CTCACGTCTGGGGAGTGGGGCTGCTGTCCAATCCCAGAGGCTGTCTGCTGCTCGGACCAC
  CAGCACTGCTGCCCCCAGGGCTACACGTGTGTAGCTGAGGGGCAGTGTCAGCGAGGAAGC
  GAGATCGTGGCTGGACTGGAGAAGATGCCTGCCCGCCGGGCTTCCTTATCCCACCCCAGA
  GACATCGGCTGTGACCAGCACACCAGCTGCCCGGTGGGGCAGACCTGCTGCCCGAGCCTG
  GGTGGGAGCTGGGCCTGCTGCCAGTTGCCCCATGCTGTGTGCTGCGAGGATCGCCAGCAC
  TGCTGCCCGGCTGGCTACACCTGCAACGTGAAGGCTCGATCCTGCGAGAAGGAAGTGGTC
  TCTGCCCAGCCTGCCACCTTCCTGGCCCGTAGCCCTCACGTGGGTGTGAAGGACGTGGAG
  TGTGGGGAAGGACACTTCTGCCATGATAACCAGACCTGCTGCCGAGACAACCGACAGGGC
  TGGGCCTGCTGTCCCTACCGCCAGGGCGTCTGTTGTGCTGATCGGCGCCACTGCTGTCCT
  GCTGGCTTCCGCTGCGCAGCCAGGGGTACCAAGTGTTTGCGCAGGGAGGCCCCGCGCTGG
  GACGCCCCTTTGAGGGACCCAGCCTTGAGACAGCTGCTGTGA
  
  Clone variation with respect to NM_002087.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002087 unedited
  TAGATTTTGTACACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGCGCTACCCG
  GTTGCTGCTGCCCAAGGACCGCGGAGTCGGACGCAGGCAGACCATGTGGACCCTGGTGAG
  CTGGGTGGCCTTAACAGCAGGGCTGGTGGCTGGAACGCGGTGCCCAGATGGTCAGTTCTG
  CCCTGTGGCCTGCTGCCTGGACCCCGGAGGAGCCAGCTACAGCTGCTGCCGTCCCCTTCT
  GGACAAATGGCCCACAACACTGAGCAGGCATCTGGGTGGCCCCTGCCAGGTTGATGCCCA
  CTGCTCTGCCGGCCACTCCTGCATCTTTACCGTCTCAGGGACTTCCAGTTGCTGCCCCTT
  CCCAGAGGCCGTGGCATGCGGGGATGGCCATCACTGCTGCCCACGGGGCTTCCACTGCAG
  TGCAGACGGGCGATCCTGCTTCCAAAGATCAGGTAACAACTCCGTGGGTGCCATCCAGTG
  CCCTGATAGTCAGTTCGAATGCCCGGACTTCTCCACGTGCTGTGTTATGGTCGATGGCTC
  CTGGGGGTGCTGCCCCATGCCCCAGGCTTCCTGCTGTGAAGACAGGGTGCACTGCTGTCC
  GCACGGTGCCTTCTGCGACCTGGTTCACACCCGCTGCATCACACCCACGGGCACCCACCC
  CCTGNCAAAGAAGCTCCCTGCCCAGAGGACTAACAGGGCAGTGGCCTTGTCCAGCTCGGT
  CATGTGTCCCGACGCACGGTCCCGGTGCCCTGATGGTTCTACCTGCTGTGAGCTTGCCAG
  TGGGAAGTATGGCTGCTGCCCATGCCCAAACGCCACTGCTGCTCCGATCACCTGCACTGC
  TGCCCCCAAGACACTGTGTGTGACCTGATCCANAGTTAGTGCCTCTCCAAGAGAACGCTA
  CACCGACCTCTCACTAGCTGNCTGCCAACATGGGGGGATGTGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002087 unedited
  CCCCGCGCCGCCCCTCGCCCTCCCCCCTCCCCCCTCTCTTTACTTAGTACCCGGCCCGCA
  TACTACGATCGGTTTTTTTTTTTTTTTTTTCGAAGTGTACAAACTTTTATTGAAACGCAC
  ACGCGCACACACACAAACACCCCTGTGGTTAGGGAAAAGCACCTGCCCACAGGGTCCACT
  GAAACGGGGAGGGGATGGCAGCTTGTAATGTGGCTTTTGCCACAACCCCCTTCTGACAGG
  GAAGGCCTTAGATTGAGGCCCCACCTCCCATGGTGATGGGGAGCTCACAACGGTGCCCAG
  CGAGACTTTGGTTAGGGCGAGGTGCTAGGGAGGCCTGAGCATAGGGCACCTCTCCGAGTG
  GGGTCCCCACGCCTGCACAGTCTTCAGTACCTGCATCTCACAATACCTGTCTCAAGGCTG
  GGTCCCTTAAAGGGGCGCCCCCACGCGGGGCCTCCCTGTGCACACACTAGCTCCCCCTGG
  CTGCCCCGTGCCAGCCAATAAGACCAGCTGTGCTTTCCACTCGCCCAACAAACCTCCTGT
  CTGTAGGCGACCACCACATCAGCCCTTGCCCCTTGTCTCCCCTCCCATTCCCGTCTATTT
  ATGCCCCCACGGCCCCTGCTTTAATTCCACGCCCTTTTACACCCACTCCCGGTTCCCCGC
  CCCCGAACCCCTCACGTTGTCCACACCCACTTCCTTTTCCCCAGGACCGACCCTTCCCCC
  CTCCCCCCCCACCCCCCCGAACCCAACTTTTGCCACCCCCACAACCCACCTACCCGGCCC
  CCTTGTCCCTATGCCCCCCTCCATCCCCACCTTCCCCCCCTATTTCACTTCACCCCCCCC
  ATTTTTCTCTCCCCCCCCCCCCTTCATCTCACGCGCGCGACAAAGACACATTCTTTCCTC
  CTACTCCTCCATTACCCCACCCCCCCACCCCCTTTCTTTCCCCCCTCCCGACTATAAC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002087
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002087.2, NP_002078.1
• RefSeq Size: 2323 bp
• RefSeq ORF: 1782 bp
• Locus ID: 2896
• Cytogenetics: 17q21.31
• Domains: GRAN
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).