FYN (NM_002037) Human Untagged Clone

CAT#: SC118862

FYN (untagged)-Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FYN
• Synonyms: p59-FYN; SLK; SYN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118862 sequence for NM_002037 edited (data generated by NextGen Sequencing)
  ATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAACAAAACTGACGGAGGAGAGGGACGGC
  AGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCC
  AGCTTCGGTGTGACCTCCATCCCCAACTACAACAACTTCCACGCAGCCGGGGGCCAAGGA
  CTCACCGTCTTTGGAGGTGTGAACTCTTCGTCTCATACGGGGACCTTGCGTACGAGAGGA
  GGAACAGGAGTGACACTCTTTGTGGCCCTTTATGACTATGAAGCACGGACAGAAGATGAC
  CTGAGTTTTCACAAAGGAGAAAAATTTCAAATATTGAACAGCTCGGAAGGAGATTGGTGG
  GAAGCCCGCTCCTTGACAACTGGAGAGACAGGTTACATTCCCAGCAATTATGTGGCTCCA
  GTTGACTCTATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGAAAAGATGCTGAG
  CGACAGCTATTGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGTGAAACC
  ACCAAAGGTGCCTATTCACTTTCTATCCGTGATTGGGATGATATGAAAGGAGACCATGTC
  AAACATTATAAAATTCGCAAACTTGACAATGGTGGATACTACATTACCACCCGGGCCCAG
  TTTGAAACACTTCAGCAGCTTGTACAACATTACTCAGAGAGAGCTGCAGGTCTCTGCTGC
  CGCCTAGTAGTTCCCTGTCACAAAGGGATGCCAAGGCTTACCGATCTGTCTGTCAAAACC
  AAAGATGTCTGGGAAATCCCTCGAGAATCCCTGCAGTTGATCAAGAGACTGGGAAATGGG
  CAGTTTGGGGAAGTATGGATGGGTACCTGGAATGGAAACACAAAAGTAGCCATAAAGACT
  CTTAAACCAGGCACAATGTCCCCCGAATCATTCCTTGAGGAAGCGCAGATCATGAAGAAG
  CTGAAGCACGACAAGCTGGTCCAGCTCTATGCAGTGGTGTCTGAGGAGCCCATCTACATC
  GTCACCGAGTATATGAACAAAGGAAGTTTACTGGATTTCTTAAAAGATGGAGAAGGAAGA
  GCTCTGAAATTACCAAATCTTGTGGACATGGCAGCACAGGTGGCTGCAGGAATGGCTTAC
  ATCGAGCGCATGAATTATATCCATAGAGATCTGCGATCAGCAAACATTCTAGTGGGGAAT
  GGACTCATATGCAAGATTGCTGACTTCGGATTGGCCCGATTGATAGAAGACAATGAGTAC
  ACAGCAAGACAAGGTGCAAAGTTCCCCATCAAGTGGACGGCCCCCGAGGCAGCCCTGTAC
  GGGAGGTTCACAATCAAGTCTGACGTGTGGTCTTTTGGAATCTTACTCACAGAGCTGGTC
  ACCAAAGGAAGAGTGCCATACCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAG
  CGAGGCTACAGGATGCCCTGCCCGCAGGACTGCCCCATCTCTCTGCATGAGCTCATGATC
  CACTGCTGGAAAAAGGACCCTGAAGAACGCCCCACTTTTGAGTACTTGCAGAGCTTCCTG
  GAAGACTACTTTACCGCGACAGAGCCCCAGTACCAACCTGGTGAAAACCTGTAA
  
  Clone variation with respect to NM_002037.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002037 unedited
  TCGGCACGAGGGTTGTGCGAGCGGCGCGTCCTCTCCCGCCCGGGCGCGCCGCGCTTCTCC
  CAGCGCACCGAGGACCGCCCGGGCGCACACAAAGCCGCCGCCCGCGCCGCACCGCCCGGC
  GGCCGCCGCCCGCGCCAGGGAGGGATTCGGCCGCCGGGCCGGGGACACCCCGGCGCCGCC
  CCCTCGGTGCTCTCGGAAGGCCCACCGGCTCCCGGGCCCGCCGGGGACCCCCCGGAGCCG
  CCTCGGCCGCGCCGGAGGAGGGCGGGGAGAGGACCATGTGAGTGGGCTCCGGAGCCTCAG
  CGCCGCGCAGTTTTTTTGAAGAAGCAGGATGCTGATCTAAACGTGGAAAAAGACCAGTCC
  TGCCTCTGTTGTAGAAGACATGTGGTGTATATAAAGTTTGTGATCGTTGGCGGAAATTTT
  GGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAACAAAACTGACGGAG
  GAGAGGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCT
  CAGCACTACCCCAGCTTCGGTGTGACCTCCATCCCCAACTACAACAACTTCCACGCAGCC
  GGNGGCCAAGGACTCACCGTCTTTGGAGGTGTGAACTCTTCGTCTCATACGGGGACCTTG
  CGTACGAGAGGAGGAACAGGAGTGACACTCTNTGTGGCCCTTTATGACTATGAAGCACGG
  ACAGAAGATGACCTGAGTTNTCACAAAGGGAGAAAAAATTCAATATTGAACAGCTCGAAN
  GGAGATGGTGGGAAGCCCGCTCCTTGACACTGGAGAGAAGGGTACATTCCAGCATTATGT
  GGCTCCAGTTGACTCTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002037 unedited
  CACTGGGGGCAGGGGTACACAGGGCATGCCACTCCGGGTATCTGTTCAGGAAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGTTGAATCAGGTGA
  AGACAGAGTTAAAATCACATAGGATTTGCATTTTTAAAAAAGGAAAGCACTAGGATTGTT
  GGCACTGAAGTAACTATTTACACTGAACAGAGGTTTGGCCTTTTACATAACATCGATACA
  ATGCATTTTCCAAAGTCTGAGAAATAACAAGGTTCTGTCTCGAATGCTTCACAGAGGAGG
  TTCNGATTTGGGGACAAGTGTTATTAAAGAAGGCCGAGGAAGCTCCAGAACATCATAGTG
  AGACGCAATCAAAAAACAAGCAATATACGAGCAAAATGAAAAAGAAGGAGAAAGGGGGAG
  GGCGCAGCAGACGGACCACTCCAAAACAAAACCAAAAAGGAAAGAAACAAATAGGGCGGA
  CGCAAGAAATTGAGCGATATAGAGGGGAGATAGTAAAAAAAAAGGCCCCGCAGAAGGAAC
  AGAAAATCGTCGCAAGCGACCCGAAACACGAACAGTAAATAGAGAAGACGAAAAGGGGAC
  CCGAACGGCGGATCATGGAGAATAGTATAAGAAGAAGGACAAAAGAAGACAGTCAAACAG
  AAAGCAACGAGGCCGCAGAAGTACCCAAGATGCGGAGTGCGGCGGGCGTGGGAAAAGAAA
  GATCGGGAGTGGTGGAGAAGAAATGAGGGGGCTATAGAGAGTGAACTCAGCCGAAAAAGA
  AGTACGCGACTCCACGAACTAAAACAATCCAGCGGGCATGCAGAGACCGGGCAAGTTACC
  GAAACAGTATACGATTGTGTCTTCAGATGTATCGTGAATAAAAGACGATAGCAGAACATA
  CGACACAAGACTCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002037
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002037.3, NP_002028.1
• RefSeq Size: 2650 bp
• RefSeq ORF: 1614 bp
• Locus ID: 2534
• Cytogenetics: 6q21
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis
• Gene Summary: 'This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.