FYN (NM_002037) Human Untagged Clone

CAT#: SC323428

FYN (untagged)-Kinase deficient mutant (K299M) of Human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FYN
• Synonyms: p59-FYN; SLK; SYN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002037, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAACAAAACTGACGGAGGAGAGGGACGGCAGCCTGAACC
  AGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCCAGCTTCGGTGTGACCTCCAT
  CCCCAACTACAACAACTTCCACGCAGCCGGGGGCCAAGGACTCACCGTCTTTGGAGGTGTGAACTCTTCG
  TCTCATACGGGGACCTTGCGTACGAGAGGAGGAACAGGAGTGACACTCTTTGTGGCCCTTTATGACTATG
  AAGCACGGACAGAAGATGACCTGAGTTTTCACAAAGGAGAAAAATTTCAAATATTGAACAGCTCGGAAGG
  AGATTGGTGGGAAGCCCGCTCCTTGACAACTGGAGAGACAGGTTACATTCCCAGCAATTATGTGGCTCCA
  GTTGACTCTATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGAAAAGATGCTGAGCGACAGCTAT
  TGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGTGAAACCACCAAAGGTGCCTATTCACT
  TTCTATCCGTGATTGGGATGATATGAAAGGAGACCATGTCAAACATTATAAAATTCGCAAACTTGACAAT
  GGTGGATACTACATTACCACCCGGGCCCAGTTTGAAACACTTCAGCAGCTTGTACAACATTACTCAGAGA
  GAGCTGCAGGTCTCTGCTGCCGCCTAGTAGTTCCCTGTCACAAAGGGATGCCAAGGCTTACCGATCTGTC
  TGTCAAAACCAAAGATGTCTGGGAAATCCCTCGAGAATCCCTGCAGTTGATCAAGAGACTGGGAAATGGG
  CAGTTTGGGGAAGTATGGATGGGTACCTGGAATGGAAACACAAAAGTAGCCATAAAGACTCTTAAACCAG
  GCACAATGTCCCCCGAATCATTCCTTGAGGAAGCGCAGATCATGAAGAAGCTGAAGCACGACAAGCTGGT
  CCAGCTCTATGCAGTGGTGTCTGAGGAGCCCATCTACATCGTCACCGAGTATATGAACAAAGGAAGTTTA
  CTGGATTTCTTAAAAGATGGAGAAGGAAGAGCTCTGAAATTACCAAATCTTGTGGACATGGCAGCACAGG
  TGGCTGCAGGAATGGCTTACATCGAGCGCATGAATTATATCCATAGAGATCTGCGATCAGCAAACATTCT
  AGTGGGGAATGGACTCATATGCAAGATTGCTGACTTCGGATTGGCCCGATTGATAGAAGACAATGAGTAC
  ACAGCAAGACAAGGTGCAAAGTTCCCCATCAAGTGGACGGCCCCCGAGGCAGCCCTGTACGGGAGGTTCA
  CAATCAAGTCTGACGTGTGGTCTTTTGGAATCTTACTCACAGAGCTGGTCACCAAAGGAAGAGTGCCATA
  CCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAGCGAGGCTACAGGATGCCCTGCCCGCAGGAC
  TGCCCCATCTCTCTGCATGAGCTCATGATCCACTGCTGGAAAAAGGACCCTGAAGAACGCCCCACTTTTG
  AGTACTTGCAGAGCTTCCTGGAAGACTACTTTACCGCGACAGAGCCCCAGTACCAACCTGGTGAAAACCT
  GTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002037 unedited
  ACCGCCCGTCCAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTTTTTGAA
  GAAGCAGGATGCTGACTCTAAACGTGGAAAAAGACCAGTCCTGCCTCTGTTGTAGAAGACATGTGGTGTA
  TATAAAGCTTGTGATCGTTGGCGGAAATTTTGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAA
  GAAGCAACAAAACTGACGGAGGAGAGGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAG
  ACCCCACCCCTCAGCACTACCCCCAGCCTTCGGGTGGTGACCTCCATCCCCAACTACAACCAACTTTCCA
  CGCCAGCCCGGGGGCAAAGGACTCCCCGTCTTTGGAGGGTGTGAACCTCTTCTTCTATTACGGGAACCTT
  GCGTACAAAAGGAGGAACAGAGATTGACACTCTTGGTGGCCCTTTATAACAATGAGGAACGGACGGAGAA
  TGACTGGAGTTTTCAAAAAGGAAAAAAATTCAAATATTTAAAAGCCCTGGAAAGAGATTGGGGGGGAACC
  CCGCTCTCTGACCAATGGGGAGACACGTTACATTTCCAGCGCATATTGTGGGGCCCCGTTAGACTCTACC
  CACGCAGAGAGGTGACACTTTGAAACCTCTGCCAAAGATGCTGAACACAACTCTTGTGCTCTTTGAACCC
  AGAGAGCCTTCTTATACGCGAGATGAGAACACCCAAGTGGCCATACACTTCTACCTGATTGGATGATGAA
  AGGAACACTGTCACACTATAAATCCCACTTGACATGTGTATCACGTAACCGCAGTGACCTCACGACTTGC
  CAATCCAAGGTCAGTCTGCGCGAATTCGTCAGATGCAGCTA
  
  Kinase domain sequence

  >SC323428 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GTGWCAGAGMTGGGAATGGGCAGTTTGGGGAAGTATGGATGGGTACCTGGAATGGAAACACAAAAGTAGC
  CATAATGACTCTTAAACCAGGCACAATGTCCCCCGAATCATTCCTTGAGGAAGCGCAGATCATGAAGAAG
  CTGAAGCACGACAAGCTGGTCCAGCTCTATGCAGTGGTGTCTGAGGAGCCCATCTACATCGTCACCGAGT
  ATATGAACAAAGGAAGTTTACTGGATTTCTTAAAAGATGGAGAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_002037
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002037.3, NP_002028.1
• RefSeq Size: 2650 bp
• RefSeq ORF: 1614 bp
• Locus ID: 2534
• Cytogenetics: 6q21
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis
• Gene Summary: 'This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.