Glutaredoxin 1 (GLRX) (NM_002064) Human Untagged Clone

CAT#: SC118879

GLRX (untagged)-Human glutaredoxin (thioltransferase) (GLRX), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLRX
• Synonyms: GRX; GRX1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002064 edited
  ATGGCTCAAGAGTTTGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAG
  CCCACCTGCCCGTACTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAA
  GGGCTTCTGGAATTTGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTAT
  TTGCAACAGCTCACGGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATA
  GGCGGATGCAGTGATCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAG
  CAGATTGGAGCTCTGCAGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002064 unedited
  TCACCATTTTGTAATACGACTTACTATTANNGGCGGCCGCGCAATTCGGCACGAGGGCAA
  TACCTGCAACTGAGGATTCTTCCCGGGGAGACCGCAGCCCATCGGCATGGCTCAAGAGTT
  TGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAGCCCACCTGCCCGTA
  CTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAAGGGCTTCTGGAATT
  TGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTATTTGCAACAGCTCAC
  GGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATAGGCGGATGCAGTGA
  TCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAGCAGATTGGAGCTCT
  GCAGTAACCACAGGTGAGTGGCAGGTGAGGTGCTGGGCCAGGAACATGGGAGACCGTGGA
  ATTCTTCTGTCGTGGTTTGTTATACTGTATGACAGCATTCTGGTTGGGGGAAGAAATGAT
  TTGGGGTGAGTTGAGGAACGGTGATGTCAATCACTTATGAATTTCACACTTTACANAGAG
  ATCGTGTCAATGAGAGAAATCCTTTCACTCAAGCTTCATTTAAGAAATATTTATTAAGCA
  TGTGCCAGACACATGAGAATATAATGATGCATAAAATGATAACCCTGGACTTATATTTTA
  GCAGAGTAGACCGATCAAAAACAAGCAAATTCTGCAATAGAATAGAATAATGTGACAGAA
  TATGGCATGGGGTGGCGGGGAAAATTGAGCTAACATGGTAAAGGGAGGCCCCTTCCTCAC
  GAAAGGGTTGAGCTTGACACCTGGTTTACCAAGGGGACTGGCTTTCCAAACATGAAGCAC
  AAGAGCTTTTTCAGCCATAGGGAATAACCAGTGCGAAAGCTTCTGAGCTGCAACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002064 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATCTATAGGTTTTTTTTA
  TTAAAAAAAAAATTAAGTGTTGTCACTAGAGATTGAAGGAAATTAACAATCTTACAATTC
  TAGAACAGGCAAACTTCATGATAAGGGATTCTTAAGTTTTCAAAGTGTAGGAGCTCTTTC
  ACAGATACACTCTTGGTGTAGGGGGCTGTAAGTTTTATCAGATGTTTTACATTTCATGCT
  TTAATCTTTGCTGGTAGTCTAAATAAAATAAATACACAGTGTTCAGGATTATCTGGATAG
  CCATCTTAAATAACAGAGTGTTCCGCATGACCACAGCCTCTGCACTTGTGCCTCTGATCC
  ATATGACACCAAGACCAACTATGCTTTTCATATGATTTCTCCAATTGGGTCCTGTGACCT
  TTCACAGAATTGTTGAACATTTCCTATGAGATCTGTGGAGGACAAGGTGGAGAACACTGG
  TTCAGAGCAGTGATTTTCAAGGTGGGATGGGATGGGGTGGAGTTGAGTGGAGAGATGCAT
  GTATTTACAAAAGACACATTCAAAATACCCACTGATTTCATAATACAAACTCCCTAAAGC
  CCAACAGCAAAATGATTTTAGCTAATGGGCCAGAGCAAATAACCAACTGTGGACTCATCA
  TTGTGGAAAAAGGACCCTGGATAAACCATATTGAAAAACCCTGTCCTAAAGGTAGAAACC
  TGCCGACCCTGGCAGAACAGTAGAAACCCCTGCCCAAGAAGAACATTGGAGGTCACGGAG
  CCGATCCTGTGCCAACACTGTCCAGTCTTTTGGAAACCAAGTCCCCTCCCCAATAACACC
  AAGTCGGCCCCCCACCTTTTGCCCCTCGCAAAAAAAAACCCGCCCCCTTTTAAACCTGTC
  CAACAAGGAAAACGCCGTTTGCCCCCTATTCTTCTCCCAAAGGTCTTTCCTTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002064
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002064.1, NP_002055.1
• RefSeq Size: 1328 bp
• RefSeq ORF: 321 bp
• Locus ID: 2745
• Cytogenetics: 5q15
• Domains: glutaredoxin
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein.