FGF2 (NM_002006) Human Untagged Clone

CAT#: SC118884

FGF2 (untagged)-Human fibroblast growth factor 2 (basic) (FGF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGF2
• Synonyms: BFGF; FGF-2; FGFB; HBGF-2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002006 edited
  GAATTCGGCACGAGGGGAGGGAGGAGAACTGGGGGCGCGGGAGGCTGGTGGGTGTGGGGG
  GTGGAGATGTAGAAGATGTGACGCCGCGGCCCGGCGGGTGCCAGATTAGCGGACGCGGTG
  CCCGCGGTTGCAACGGGATCCCGGGCGCTGCAGCTTGGGAGGCGGCTCTCCCCAGGCGGC
  GTCCGCGGAGACACCCATCCGTGAACCCCAGGTCCCGGGCCGCCGGCTCGCCGCGCACCA
  GGGGCCGGCGGACAGAAGAGCGGCCGAGCGGCTCGAGGCTGGGGGACCGCGGGCGCGGCC
  GCGCGCTGCCGGGCGGGAGGCTGGGGGGCCGGGGCCGGGGCCGTGCCCCGGAGCGGGTCG
  GAGGCCGGGGCCGGGGCCGGGGGACGGCGGCTCCCCGCGCGGCTCCAGCGGCTCGGGGAT
  CCCGGCCGGGCCCCGCAGGGACCATGGCAGCCGGGAGCATCACCACGCTGCCCGCCTTGC
  CCGAGGATGGCGGCAGCGGCGCCTTCCCGCCCGGCCACTTCAAGGACCCCAAGCGGCTGT
  ACTGCAAAAACGGGGGCTTCTTCCTGCGCATCCACCCCGACGGCCGAGTTGACGGGGTCC
  GGGAGAAGAGCGACCCTCACATCAAGCTACAACTTCAAGCAGAAGAGAGAGGAGTTGTGT
  CTATCAAAGGAGTGTGTGCTAACCGTTACCTGGCTATGAAGGAAGATGGAAGATTACTGG
  CTTCTAAATGTGTTACGGATGAGTGTTTCTTTTTTGAACGATTGGAATCTAATAACTACA
  ATACTTACCGGTCAAGGAAATACACCAGTTGGTATGTGGCACTGAAACGAACTGGGCAGT
  ATAAACTTGGATCCAAAACAGGACCTGGGCAGAAAGCTATACTTTTTCTTCCAATGTCTG
  CTAAGAGCTGATTTTAATGGCCACATCTAATCTCATTTCACATGAAAGAAGAAGTATATT
  TTAGAAATTTGTTAATGAGAGTAAAAGAAAATAAATGTGTATAGCTCAGTTTGGATAATT
  GGTCAAACAATTTTTTATCCAGTAGTAAAATATGTAACCATTGTCCCAGTAAAGAAAAAT
  AACAAAAGTTGTAAAATGTATATTCTCCCTTTTATATTGCATCTGCTGTTACCCAGTGAA
  GCTTACCTAGAGCAATGATCTTTTTCACGCATTTGCTTTATTCGAAAAGAGGCTTTTAAA
  ATGTGCATGTTTAGAAACAAAATTTCTTCATGGAAATCATATACATTAGAAAATCACAGT
  CAGATGTTTAATCAATCCAAAATGTCCACTATTTCTTATGTCATTCGTTAGTCTACATGT
  TTCTAAACATATAAATGTGAATTTAATCAATTCCTTTCATAGTTTTATAATTCTCTGGCA
  GTTCCTTATGATAGAGTTTATAAAACAGTCCTGTGTAAACTGCTGGAAGTTCTTCCACAG
  TCAGGTCAATTTTGTCAAACCCTTCTCTGTACCCATACAGCAGCAGCCTAGCAACTCTGC
  TGGTGATGGGAGTTGTATTTTCAGTCTTCGCCAGGTCATTGAGATCCATCCACTCACATC
  TTAAGCATTCTTCCTGGCAAAAATTTATGGTGAATGAATATGGCTTTAGGCGGCAGATGA
  TATACATATCTGACTTCCCAAAAGCTCCAGGATTTGTGTGCTGTTGCCGAATACTCAGGA
  CGGACCTGAATTCTGATTTTATACCAGTCTCTTCAAAAACTTCTCGAACCGCTGTGTCTC
  CTACGTAAAAAAAGAGATGTACAAATCAATAATAATTACACTTTTAGAAACTGTATCATC
  AAAGATTTTCAGTTAAAGTAGCATTATGTAAAGGCTCAAAACATTACCCTAACAAAGTAA
  AGTTTTCAATACAAATTCTTTGCCTTGTGGATATCAAGAAATCCCAAAATATTTTCTTAC
  CACTGTAAATTCAAGAAGCTTTTGAAATGCTGAATATTTCTTTGGCTGCTACTTGGAGGC
  TTATCTACCTGTACATTTTTGGGGTCAGCTCTTTTTAACTTCTTGCTGCTCTTTTTCCCA
  AAAGGTAAAAATATAGATTGAAAAGTTAAAACATTTTGCATGGCTGCAGTTCCTTTGTTT
  CTTGAGATAAGATTCCAAAGAACTTAGATTCATTTCTTCAACACCGAAATGCTGGAGGTG
  TTTGATCAGTTTTCAAGAAACTTGGAATATAAATAATTTTATAATTCAACAAAGGTTTTC
  ACATTTTATAAGGTTGATTTTTCAATTAAATGCAAATTTATGTGGCAGGATTTTTATTGC
  CATTAACATATTTTTGTGGCTGCTTTTTCTACACATCCAGATGGTCCCTCTAACTGGGCT
  TTCTCTAATTTTGTGATGTTCTGTCATTGTCTCCCAAAGTATTTAGGAGAAGCCCTTTAA
  AAAGCTGCCTTCCTCTACCACTTTGCTGGAAAGCTTCACAATTGTCACAGACAAAGATTT
  TTGTTCCAATACTCGTTTTGCCTCTATTTTTCTTGTTTGTCAAATAGTAAATGATATTTG
  CCCTTGCAGTAATTCTACTGGTGAAAAACATGCAAAGAAGAGGAAGTCACAGAAACATGT
  CTCAATTCCCATGTGCTGTGACTGTAGACTGTCTTACCATAGACTGTCTTACCCATCCCC
  TGGATATGCTCTTGTTTTTTCCCTCTAATAGCTATGGAAAGATGCATAGAAAGAGTATAA
  TGTTTTAAAACATAAGGCATTCGTCTGCCATTTTTCAATTACATGCTGACTTCCCTTACA
  ATTGAGATTTGCCCATAGGTTAAACATGGTTAGAAACAACTGAAAGCATAAAAGAAAAAT
  CTAGGCCGGGTGCAGTGGCTCATGCCTATATTCCCTGCACTTTGGGAGGCCAAAGCAGGA
  GGATCGCTTGAGCCCAGGAGTTCAAGACCAACCTGGTGAAACCCCGTCTCTACAAAAAAA
  CACAAAAAATAGCCAGGCATGGTGGCGTGTACATGTGGTCTCAGATACTTGGGAGGCTGA
  GGTGGGAGGGTTGATCACTTGAGGCTGAGAGGTCAAGGTTGCAGTGAGCCATAATCGTGC
  CACTGCAGTCCAGCCTAGGCAACAGAGTGAGACTTTGTCTCAAAAAAAGAGAAATTTTCC
  TTAATAAGAAAAGTAATTTTTACTCTGATGTGCAATACATTTGTTATTAAATTTATTATT
  TAAGATGGTAGCACTAGTCTTAAATTGTATAAAATATCCCCTAACATGTTTAAATGTCCA
  TTTTTATTCATTATGCTTTGAAAAATAATTATGGGGAAATACATGTTTGTTATTAAATTT
  ATTATTAAAGATAGTAGCACTAGTCTTAAATTTGATATAACATCTCCTAACTTGTTTAAA
  TGTCCATTTTTATTCTTTATGTTTGAAAATAAATTATGGGGATCCTATTTAGCTCTTAGT
  ACCACTAATCAAAAGTTCGGCATGTAGCTCATGATCTATGCTGTTTCTATGTCGTGGAAG
  CACCGGATGGGGGTAGTGAGCAAATCTGCCCTGCTCAGCAGTCACCATAGCAGCTGACTG
  AAAATCAGCACTGCCTGAGTAGTTTTGATCAGTTTAACTTGAATCACTAACTGACTGAAA
  ATTGAATGGGCAAATAAGTGCTTTTGTCTCCAGAGTATGCGGGAGACCCTTCCACCTCAA
  GATGGATATTTCTTCCCCAAGGATTTCAAGATGAATTGAAATTTTTAATCAAGATAGTGT
  GCTTTATTCTGTTGTATTTTTTATTATTTTAATATACTGTAAGCCAAACTGAAATAACAT
  TTGCTGTTTTATAGGTTTGAAGAACATAGGAAAAACTAAGAGGTTTTGTTTTTATTTXXX
  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002006 unedited
  ACATTTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGAGGGAGGAG
  AACTGGGGGCGCGGGAGGCTGGTGGGTGTGGGGGGTGGAGATGTAGAAGATGTGACGCCG
  CGGCCCGGCGGGTGCCAGATTAGCGGACGCGGTGCCCGCGGTTGCAACGGGATCCCGGGC
  GCTGCAGCTTGGGAGGCGGCTCTCCCCAGGCGGCGTCCGCGGAGACACCCATCCGTGAAC
  CCCAGGTCCCGGGCCGCCGGCTCGCCGCGCACCAGGGGCCGGCGGACAGAAGAGCGGCCG
  AGCGGCTCGAGGCTGGGGGACCGCGGGCGCGGCCGCGCGCTGCCGGGCGGGAGGCTGGGG
  GGCCGGGGCCGGGGCCGTGCCCCGGAGCGGGTCGGAGGCCGGGGCCGGGGCCGGGGGACG
  GCGGCTCCCCGCGCGGCTCCAGCGGCTCGGGGATCCCGGCCGGGCCCCGCAGGGACCATG
  GCAGCCGGGAGCATCACCACGCTGCCCGCCTTGCCCGAGGATGGCGGCAGCGGCGCCTTC
  CCGCCCGGCCACTTCAAGGACCCCAAGCGGCTGTACTGCAAAAACGGNGGCTTCTTCCTG
  CGCATCCACCCCGACGGCCGAGTTGACTGGGTCCGGGAGAAGAGCGACCCTCACATCAAG
  CTACAACTTTAAGCAGAAGAGAGAGGAGTTGTGTCTATCAAATGAGTGTGTGCTAACCGT
  TACCTGGCTATGAACGAAGAATGGAAGATTACTGGCTTCTAAAGTGTTACCGATGAGTGT
  TTCTCTTTTCACCGATCGGAATCTATTACTACAATCTTTCCCGTCAAGGAATACACCATT
  TGGTTTGTGGCACTGAACAAACTGCGCGCTTAACTCGGATCCAAAAGGAACTGGGCAGAA
  AGCTTTCTTTTTCTTCCAAGTTGCTAAAACCTATTTAATGGCCCCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002006 unedited
  TGTTGTTTTTCATCCTAATTCTATTATTAAATATAACAAAATAGAAATAACAAATATAAA
  CTCCATTTCATTATTGTCCTGTAACTAAACAAAACAATACAACATATTTAAACATATCTC
  TTCATCAGCAAAAATAAAAACAAAACCTCTTAGTTTTTCCTATGTTCTTCAAACCTATAA
  AACAGCAAATGTTATTTCAGTTTGGCTTACAGTATATTAAAATAATAAAAAATACAACAG
  AATAAAGCACACTATCTTGATTAAAAATTTCAATTCATCTTGAAATCCTTGGGGAAGAAA
  TATCCATCTTGAGGTGGAAGGGTCTCCCGCATACTCTGGAGACAAAAGCACTTATTTGCC
  CATTCAATTTTCAGTCAGTTAGTGATTCAAGTTAAACTGATCAAAACTACTCAGGCAGTG
  CTGATTTTCAGTCAGCTGCTATGGTGACTGCTGAGCAGGGCAGATTTGCTCACTACCCCC
  ATCCGGTGCTTCCACGACATAGAAACAGCATAGATCATGAGCTACATGCCGAACTTTTGA
  TTAGTGGTACTAAGAGCTAAATAGGATCCCCATAATTTATTTTCAAACATAAAGAATAAA
  AATGGACATTTAAACAAGTTAGGAGATGTTATATCAAATTTAAGACTAGTGCTACTATCT
  CTAATAATAAATTTAATAACAAACATGTATCTCCCCATAATTATCTTTCACAGCATAATG
  AATAAAAATGGACATTTAAACATGTTAGGGGATATTTTATACAATTTAAGACTAGTGCTA
  CCATCTTAAATATAAATTTATAACACTGTATTGCACATCAGAGTAAAAATACTTTTCTAT
  AAGGGAAATTTCTCTTTTTTGAGACAAGTCTACTCTGTGGCTAGCCTGACTGCAATGCAC
  GATATTGCTCACTGCACTCTGACTCTCAGCCCCAGGATTACCCTTCCACTCACGCTCCAA
  GGATTGNAACACATGCCCNCCACT
  
• Restriction Sites: Please inquire
• ACCN: NM_002006
• Insert Size: 3570 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002006.3, NP_001997.4
• RefSeq Size: 6803 bp
• RefSeq ORF: 867 bp
• Locus ID: 2247
• Cytogenetics: 4q28.1
• Domains: FGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This transcript may encode multiple distinct isoforms through the use of alternative translation start codons (PMID: 17131363). This record represents use of the 5' most non-AUG start codon and encodes the longest (34-kDa) isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.