DBT (NM_001918) Human Untagged Clone

CAT#: SC118943

DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBT
• Synonyms: BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001918, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGCAGTCCGTATGCTGAGAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTTCGCTATTTTC
  AAACATGTGGTAATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTTGGTTATCCTTCATTCAAGTA
  TAGTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTCCGTGGACAGGTTGTTCAGTTCAAGCTCTCA
  GACATTGGAGAAGGGATTAGAGAAGTAACTGTTAAAGAATGGTATGTAAAAGAAGGAGATACAGTGTCTC
  AGTTTGATAGCATCTGTGAAGTTCAAAGTGATAAAGCTTCTGTTACCATCACTAGTCGTTATGATGGAGT
  CATTAAAAAACTCTATTATAATCTAGACGATATTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACG
  GAAGCTTTAAAAGATTCAGAAGAAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAACATACACACC
  AAGAGATAAAGGGCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTGGCAATGGAAAACAATATTAA
  GCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTAAAGAAGATATCCTCAACTATTTGGAA
  AAGCAGACAGGAGCTATATTGCCTCCTTCACCCAAAGTTGAAATTATGCCACCTCCACCAAAGCCAAAAG
  ACATGACTGTTCCTATACTAGTATCAAAACCTCCGGTATTCACAGGCAAAGACAAAACAGAACCCATAAA
  AGGCTTTCAAAAAGCAATGGTCAAGACTATGTCTGCAGCCCTGAAGATACCTCATTTTGGTTATTGTGAT
  GAGATTGACCTTACTGAACTGGTTAAGCTCCGAGAAGAATTAAAACCCATTGCATTTGCTCGTGGAATTA
  AACTCTCCTTTATGCCTTTCTTCTTAAAGGCTGCTTCCTTGGGATTACTACAGTTTCCTATCCTTAACGC
  TTCTGTGGATGAAAACTGCCAGAATATAACATATAAGGCTTCTCATAACATTGGGATAGCAATGGATACT
  GAGCAGGGTTTGATTGTCCCTAATGTGAAAAATGTTCAGATCTGCTCTATATTTGACATCGCCACTGAAC
  TGAACCGCCTCCAGAAATTGGGCTCTGTGGGTCAGCTCAGCACCACTGATCTTACAGGAGGAACATTTAC
  TCTTTCCAACATTGGATCAATTGGTGGTACCTTTGCCAAACCAGTGATAATGCCACCTGAAGTAGCCATT
  GGGGCCCTTGGATCAATTAAGGCCATTCCCCGATTTAACCAGAAAGGAGAAGTATATAAGGCACAGATAA
  TGAATGTGAGCTGGTCAGCTGATCACAGAGTTATTGATGGTGCTACAATGTCACGCTTCTCCAATTTGTG
  GAAATCCTATTTAGAAAACCCAGCTTTTATGCTACTAGATCTGAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001918 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCAGTCCGTATGCTGA
  GAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTTCGCTATTTTCAAACATGTGGTA
  ATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTTGGTTATCCTTCATTCAAGTATA
  GTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTCCGTGGACAGGTTGTTCAGTTCA
  AGCTCTCAGACATTGGAGAAGGGATTAGAGAAGTAACTGTTAAAGAATGGTATGTAAAAG
  AAGGAGATACAGTGTCTCAGTTTGATAGCATCTGTGAAGTTCAAAGTGATAAAGCTTCTG
  TTACCATCACTAGTCGTTATGATGGAGTCATTAAAAAACTCTATTATAATCTAGACGATA
  TTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACGGAAGCTTTAAAAGATTCAGAAG
  AAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAACATACACACCAAGAGATAAAGG
  GCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTGGCAATGGAAAACAATATTAAGC
  TGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTANAGAAGATATCCTCAACT
  ATTTGGAAAAGCAGACAGGAGCTATATTGCCTNCTTCACCCAAGTTGAAATATGCCACCT
  NCACAAAGCCAAAGACATGACTGTCCTATACTAGTATCAAAACCTCCGTATCACAGGCAA
  GACAAACAGAACCATAAAAGGCTTCAAAAGCATGGTCAAGACTTGTCTGCAGCCTGAGAT
  ACCTATTTTGGTTATGNGAGAAATGACCTACTGACTGGNTAGCTCGAAAAAATAAACCCA
  TGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001918 unedited
  AGACTCATGGTCGCTGCCGCAATCCANGATCAGTTTTTTTTTTTTTTTTTTGAGACAAAT
  GTTGCTCTGCCACCCAGGCTGGAGTGCAGTGGTGTGATCTCTGCTCACTGTAACCTCCAC
  CTCTTGGGCTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGTG
  TGCCACCAGGCCCAGGTAATTTTTTGTATTTTTACAAAACTGTAGAGATGGGGTTTCACC
  ATGTGGGCCAGGGTGGTCTCGAACTCCTGACCTCAGGTGATCTGGCCACCTGGGCCTCCC
  AAAGTGCTGGGATTACAGGCGTGAGCCACCAGGCTAATTTTAAAAATAATGTTTTTTGAG
  ATGGGATCTCACTATGTTGCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGTGATCCTCCT
  GGTTCAGCCTCCCCTATAACTGGGGATTACAGTTACAAGTCATCACACCTGGCTGATTTA
  TTCTTTCCTGATTTTAAAATTATTTCCATTTTGGGAAAAACCAAGTTGATTTTAAAACAA
  CCTTTTTTCTGGCACAAAACTTGCCTTAAACAACTAATTTTGGCATACAGGTTTCAACTG
  GACCAAATTAGAAACAACTGGGATTTCCGAAAGCCAATTGGAATGGTGGAACAATTATCC
  CGGTCCTTTTTCTGAGTAAGTGGACCTTTTACAAAATGGTTCTTTGTGAAACCTTCGTTT
  CCATACAAAAAGTCTCATTTTTTTTATCCTTAATACCATTAAGCTTTTCCTTCTCCTGGT
  AAGGAACATTTTACCCCGGCATCTGGGTAAATGAATAGACCCTAATTTCATAAACCGATT
  TTTAAGGCGGAGACCCCATAAGTTAATATGGGAGTGCGCTTCTCTCATTTTTTTGATAAA
  AACAAAATCGGTGGGTGCCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001918
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001918.1, NP_001909.1
• RefSeq Size: 10831 bp
• RefSeq ORF: 1449 bp
• Locus ID: 1629
• Cytogenetics: 1p21.2
• Domains: biotin_lipoyl, 2-oxoacid_dh, e3_binding
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: 'The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'