DBT (NM_001918) Human Untagged Clone

CAT#: SC118943

DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein


  "NM_001918" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DBT
Synonyms BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001918, the custom clone sequence may differ by one or more nucleotides


ATGGCTGCAGTCCGTATGCTGAGAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTTCGCTATTTTC
AAACATGTGGTAATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTTGGTTATCCTTCATTCAAGTA
TAGTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTCCGTGGACAGGTTGTTCAGTTCAAGCTCTCA
GACATTGGAGAAGGGATTAGAGAAGTAACTGTTAAAGAATGGTATGTAAAAGAAGGAGATACAGTGTCTC
AGTTTGATAGCATCTGTGAAGTTCAAAGTGATAAAGCTTCTGTTACCATCACTAGTCGTTATGATGGAGT
CATTAAAAAACTCTATTATAATCTAGACGATATTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACG
GAAGCTTTAAAAGATTCAGAAGAAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAACATACACACC
AAGAGATAAAGGGCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTGGCAATGGAAAACAATATTAA
GCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTAAAGAAGATATCCTCAACTATTTGGAA
AAGCAGACAGGAGCTATATTGCCTCCTTCACCCAAAGTTGAAATTATGCCACCTCCACCAAAGCCAAAAG
ACATGACTGTTCCTATACTAGTATCAAAACCTCCGGTATTCACAGGCAAAGACAAAACAGAACCCATAAA
AGGCTTTCAAAAAGCAATGGTCAAGACTATGTCTGCAGCCCTGAAGATACCTCATTTTGGTTATTGTGAT
GAGATTGACCTTACTGAACTGGTTAAGCTCCGAGAAGAATTAAAACCCATTGCATTTGCTCGTGGAATTA
AACTCTCCTTTATGCCTTTCTTCTTAAAGGCTGCTTCCTTGGGATTACTACAGTTTCCTATCCTTAACGC
TTCTGTGGATGAAAACTGCCAGAATATAACATATAAGGCTTCTCATAACATTGGGATAGCAATGGATACT
GAGCAGGGTTTGATTGTCCCTAATGTGAAAAATGTTCAGATCTGCTCTATATTTGACATCGCCACTGAAC
TGAACCGCCTCCAGAAATTGGGCTCTGTGGGTCAGCTCAGCACCACTGATCTTACAGGAGGAACATTTAC
TCTTTCCAACATTGGATCAATTGGTGGTACCTTTGCCAAACCAGTGATAATGCCACCTGAAGTAGCCATT
GGGGCCCTTGGATCAATTAAGGCCATTCCCCGATTTAACCAGAAAGGAGAAGTATATAAGGCACAGATAA
TGAATGTGAGCTGGTCAGCTGATCACAGAGTTATTGATGGTGCTACAATGTCACGCTTCTCCAATTTGTG
GAAATCCTATTTAGAAAACCCAGCTTTTATGCTACTAGATCTGAAATGA


>OriGene 5' read for NM_001918 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCAGTCCGTATGCTGA
GAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTTCGCTATTTTCAAACATGTGGTA
ATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTTGGTTATCCTTCATTCAAGTATA
GTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTCCGTGGACAGGTTGTTCAGTTCA
AGCTCTCAGACATTGGAGAAGGGATTAGAGAAGTAACTGTTAAAGAATGGTATGTAAAAG
AAGGAGATACAGTGTCTCAGTTTGATAGCATCTGTGAAGTTCAAAGTGATAAAGCTTCTG
TTACCATCACTAGTCGTTATGATGGAGTCATTAAAAAACTCTATTATAATCTAGACGATA
TTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACGGAAGCTTTAAAAGATTCAGAAG
AAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAACATACACACCAAGAGATAAAGG
GCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTGGCAATGGAAAACAATATTAAGC
TGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTANAGAAGATATCCTCAACT
ATTTGGAAAAGCAGACAGGAGCTATATTGCCTNCTTCACCCAAGTTGAAATATGCCACCT
NCACAAAGCCAAAGACATGACTGTCCTATACTAGTATCAAAACCTCCGTATCACAGGCAA
GACAAACAGAACCATAAAAGGCTTCAAAAGCATGGTCAAGACTTGTCTGCAGCCTGAGAT
ACCTATTTTGGTTATGNGAGAAATGACCTACTGACTGGNTAGCTCGAAAAAATAAACCCA
TGCT
>OriGene 3' read for NM_001918 unedited
AGACTCATGGTCGCTGCCGCAATCCANGATCAGTTTTTTTTTTTTTTTTTTGAGACAAAT
GTTGCTCTGCCACCCAGGCTGGAGTGCAGTGGTGTGATCTCTGCTCACTGTAACCTCCAC
CTCTTGGGCTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGTG
TGCCACCAGGCCCAGGTAATTTTTTGTATTTTTACAAAACTGTAGAGATGGGGTTTCACC
ATGTGGGCCAGGGTGGTCTCGAACTCCTGACCTCAGGTGATCTGGCCACCTGGGCCTCCC
AAAGTGCTGGGATTACAGGCGTGAGCCACCAGGCTAATTTTAAAAATAATGTTTTTTGAG
ATGGGATCTCACTATGTTGCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGTGATCCTCCT
GGTTCAGCCTCCCCTATAACTGGGGATTACAGTTACAAGTCATCACACCTGGCTGATTTA
TTCTTTCCTGATTTTAAAATTATTTCCATTTTGGGAAAAACCAAGTTGATTTTAAAACAA
CCTTTTTTCTGGCACAAAACTTGCCTTAAACAACTAATTTTGGCATACAGGTTTCAACTG
GACCAAATTAGAAACAACTGGGATTTCCGAAAGCCAATTGGAATGGTGGAACAATTATCC
CGGTCCTTTTTCTGAGTAAGTGGACCTTTTACAAAATGGTTCTTTGTGAAACCTTCGTTT
CCATACAAAAAGTCTCATTTTTTTTATCCTTAATACCATTAAGCTTTTCCTTCTCCTGGT
AAGGAACATTTTACCCCGGCATCTGGGTAAATGAATAGACCCTAATTTCATAAACCGATT
TTTAAGGCGGAGACCCCATAAGTTAATATGGGAGTGCGCTTCTCTCATTTTTTTGATAAA
AACAAAATCGGTGGGTGCCTAT
Restriction Sites NotI-NotI     
ACCN NM_001918
Insert Size 3670 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001918.1, NP_001909.1
RefSeq Size 10831 bp
RefSeq ORF 1449 bp
Locus ID 1629
Cytogenetics 1p21.2
Domains biotin_lipoyl, 2-oxoacid_dh, e3_binding
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Valine, leucine and isoleucine degradation
Gene Summary 'The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'

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