DBT (NM_001918) Human Untagged Clone

CAT#: SC322453

DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBT
• Synonyms: BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322453 ATGGCTGCAGTCCGTATGCTGAGAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTT
  CGCTATTTTCAAACATGTGGTAATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTT
  GGTTATCCTTCATTCAAGTATAGTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTC
  CGTGGACAGGTTGTTCAGTTCAAGCTCTCAGACATTGGAGAAGGGATTAGAGAAGTAACT
  GTTAAAGAATGGTATGTAAAAGAAGGAGATACAGTGTCTCAGTTTGATAGCATCTGTGAA
  GTTCAAAGTGATAAAGCTTCTGTTACCATCACTAGTCGTTATGATGGAGTCATTAAAAAA
  CTCTATTATAATCTAGACGATATTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACG
  GAAGCTTTAAAAGATTCAGAAGAAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAA
  CATACACACCAAGAGATAAAGGGCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTG
  GCAATGGAAAACAATATTAAGCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATA
  CTTAAAGAAGATATCCTCAACTATTTGGAAAAGCAGACAGGAGCTATATTGCCTCCTTCA
  CCCAAAGTTGAAATTATGCCACCTCCACCAAAGCCAAAAGACATGACTGTTCCTATACTA
  GTATCAAAACCTCCGGTATTCACAGGCAAAGACAAAACAGAACCCATAAAAGGCTTTCAA
  AAAGCAATGGTCAAGACTATGTCTGCAGCCCTGAAGATACCTCATTTTGGTTATTGTGAT
  GAGATTGACCTTACTGAACTGGTTAAGCTCCGAGAAGAATTAAAACCCATTGCATTTGCT
  CGTGGAATTAAACTCTCCTTTATGCCTTTCTTCTTAAAGGCTGCTTCCTTGGGATTACTA
  CAGTTTCCTATCCTTAACGCTTCTGTGGATGAAAACTGCCAGAATATAACATATAAGGCT
  TCTCATAACATTGGGATAGCAATGGATACTGAGCAGGGTTTGATTGTCCCTAATGTGAAA
  AATGTTCAGATCTGCTCTATATTTGACATCGCCACTGAACTGAACCGCCTCCAGAAATTG
  GGCTCTGTGGGTCAGCTCAGCACCACTGATCTTACAGGAGGAACATTTACTCTTTCCAAC
  ATTGGATCAATTGGTGGTACCTTTGCCAAACCAGTGATAATGCCACCTGAAGTAGCCATT
  GGGGCCCTTGGATCAATTAAGGCCATTCCCCGATTTAACCAGAAAGGAGAAGTATATAAG
  GCACAGATAATGAATGTGAGCTGGTCAGCTGATCACAGAGTTATTGATGGTGCTACAATG
  TCACGCTTCTCCAATTTGTGGAAATCCTATTTAGAAAACCCAGCTTTTATGCTACTAGAT
  CTGAAATGAAGACTGATAAGACATTCTTGAACTTTTTGAGCTTCCAAAGAGTATGTAAAC
  CCTAGCTGTGCCAGCACATGTTCATCTTTACAATTTATATTGTAAATGATTTGTATCGTA
  TGATTAAGGATCTAAGGCACAATATTTGTCACTGTTCTATTAGACTTTTTACTGAAAATG
  AATAATGGTGTAATGGTTCTCCTGGGGCTGTCACATTTTATAGGTCAGAGTGTGACTTCT
  TAATATGGTGCTGATGTTTTTGTGTCAATGGCTTGAAACTGGCAAGATTAACAAAATTAG
  GCCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCCAGGTGGGCGGAT
  CACCTGAGGTTAGAAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCTGGCCTCTACTA
  AAAATACAAAAATTAGCCGGGTGTGGTGGTGGGTACCTATAATCCCAGCTACTTGGGAGG
  CTGAGGCAGGAGAATCGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCGTGC
  TATTGCACTCCAGCCTGGGCGACAGAGCAAGACGCCATCTCAAAAACAAAAAAACAAAAT
  TCATGTTACTAAAAGACAGGTAGCCATATACAGACAGTATATGCCCTATTTTTTTTAACT
  GACTCTTAATGAAACTTTAATTTTACTTAATTAAGAAATGGAATTTATATACAAAAATAT
  TTTCCATTTCCGTTATTATGCTAATTGTTGTATGAAATAAGTGCAATTATACTTCTCTTT
  TGAGATATCCAAGAGTATATTCTTGCTCTGTATAGAGAATATCATCTGATAGTGTCTTAT
  TTATATTAATTAATGTCTTTGAAAAGGGAAAAGTATAAACTGGCCTTAAAATTGTCCAAT
  TATAGTTTTATAACCAGTCTATTAAAGGTGTTTGTTTAAAATGGAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001918
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001918.2, NP_001909.2
• RefSeq Size: 10831 bp
• RefSeq ORF: 1449 bp
• Locus ID: 1629
• Cytogenetics: 1p21.2
• Domains: biotin_lipoyl, 2-oxoacid_dh, e3_binding
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: 'The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'