DHPS (NM_001930) Human Untagged Clone

CAT#: SC118951

DHPS (untagged)-Human deoxyhypusine synthase (DHPS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHPS
• Synonyms: DHS; DS; MIG13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118951 sequence for NM_001930 edited (data generated by NextGen Sequencing)
  ATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCAC
  AGCTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTG
  AATTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGG
  CGCGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTGGAACCACTGTCACAGGAT
  GAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTTACCAGCTGCACCATTTTC
  CTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCGCTACCTTGTG
  CAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATC
  AAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAGCTCCGGGAG
  AACGGGATCAATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAG
  GACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAG
  TGGACGCCTTCTAAGATGATCGCCCGGCTGGGCAAGGAGATCAACAACCCAGAGTCCGTG
  TATTACTGGGCCCAGAAGAACCACATCCCTGTGTTTAGTCCCGCACTTACAGACGGCTCG
  CTGGGCGACATGATCTTCTTCCATTCCTACAAGAACCCGGGCCTGGTCCTGGACATCGTT
  GAGGACCTGAGGCTCATCAACACACAGGCCATCTTTGCCAAGTGCACTGGGATGATCATT
  CTGGGCGGGGGCGTGGTCAAGCACCACATTGCCAATGCCAACCTCATGCGGAACGGGGCC
  GACTACGCTGTTTACATCAACACAGCCCAGGAGTTTGATGGCTCTGACTCAGGTGCCCGA
  CCAGACGAGGCTGTCTCCTGGGGCAAGATCCGGGTGGATGCACAGCCCGTCAAGGTCTAT
  GCTGACGCCTCCCTGGTCTTCCCCCTGCTTGTGGCTGAAACCTTTGCCCAGAAGATGGAT
  GCCTTCATGCATGAGAAGAACGAGGACTGA
  
  Clone variation with respect to NM_001930.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001930 unedited
  GCAAAAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAGACGCGCGC
  GTCGGGGTTTAACGCGTTTCTGGGCCGCCGTAAGCCCGGCCTAGGGGCAGCTTTGACTCG
  AGAGCCGGCTATAGGCGCATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTG
  GCCGCCGTGCTAAAGCACAGCTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTAC
  GACTTCAACCGCGGTGTGAATTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTC
  CAAGCAACCAACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTG
  GAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTT
  ACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAG
  ACCATTCGCTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGC
  GTGGAGGAAGACCTCATCAAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGG
  GGGAAGGAGCTCCGGGAGAACGGGATCAATAGGATCGGAACCCTGGCTGGTGCCCAATGA
  GAATTACTGCCAGTTTGAGGACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCA
  GAACACAGAGGGTGTAAAGTGGACGCCTTCTAAGATGATCGCCGGCTGGGCAAGGAGATC
  AACACCCCAGAGTCGTGTATTACTGGGCCCCAGAGGACCACATCCCTGTGGTTAGTCCCG
  CACTTACAGACAGCTCGCTGGGCGACATGAACTTCTTCCATTCCTACCAGAACCCNGGGC
  TGTTCCTGGACATCGTTGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001930 unedited
  GGGGGGATGGCCCACNCNANNNTNTTCTTACACAAGAATTTGGCTTACACTACCANCTTT
  ATCTANACCGTAGCTGACCAAAAAGTAGGGAGGGGCTGGGTCTGCAAATTAATAAATAGA
  AGAGGGGGTAAGACCTTCCTGGGACCGCAGCCGCTCAGTCCTCGTTCTTCTCATGCATGA
  AGGCATCCATCTTCTGGGCAAAGGTTTCAGCCACAAGCAGGGGGAAGACCAGGGAGGCGT
  CAGCATAGACCTTGACGGGCTGTGCATCCACCCGGATCTTGCCCCAGGAGACAGCCTCGT
  CTGGTCGGGCACCTGAGTCAGAGCCATCAAACTCCTGGGCTGTGTTGATGTAAACAGCGT
  AGTCGGCCCCGTTCCGCATGAGGTTGGCATTGGCAATGTGGTGCTTGACCACGCCCCCGC
  CCAGAATGATCATCCCAGTGCACTTGGCAAAGATGGCCTGTGTGTTGATGAGCCTCAGGT
  CCTCAACGATGTCCAGGACCAGGCCCGGGTTCTTGTAGGAATGGAAGAAGATCATGTCGC
  CCAGCGAGCCGTCTGTAAGTGCGGGACTAAACACAGGGATGTGGTTCTTCTGGGCCCAGT
  AATACACGGACTCTGGGTTGTTGATCTCCTTGCCCAGCCGGGCGATCATCTTAGAAGGCG
  TCCACTTTACACCCTCTGTGTTCTGCTCCATCACCATCTGGTCCAGAATGGGCATCAGCC
  AGTCCTCAAACTTGCAGTAATTCTCATTGGGCACCAGCANGTTTCCGATCCTATTGATCC
  CGTTCTCCGGNAGCTNCTTTCCCCCTGAGCTAAACTCGCCCAAGTATGTGGGGCGCCAGG
  CACTTGATGAGGTCTTNCTNCACGCCGNCAGCTTGGGTCACCATTACGTCCACCCATGTT
  GTGCTGCACAAGGTANCGAATGGTCTCACGGATGCCCTGACTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001930
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001930.2, NP_001921.1
• RefSeq Size: 1351 bp
• RefSeq ORF: 1110 bp
• Locus ID: 1725
• Cytogenetics: 19p13.13
• Domains: DS
• Gene Summary: 'This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).