COX7B (NM_001866) Human Untagged Clone

CAT#: SC118991

COX7B (untagged)-Human cytochrome c oxidase subunit VIIb (COX7B), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COX7B
• Synonyms: APLCC; LSDMCA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118991 sequence for NM_001866 edited (data generated by NextGen Sequencing)
  ATGTTTCCCTTGGTCAAAAGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACA
  ATGGCAAGGCAGAGCCACCAGAAACGTACACCTGATTTTCATGACAAATACGGTAATGCT
  GTATTAGCTAGTGGAGCCACTTTCTGTATTGTTACATGGACATATGTAGCAACACAAGTC
  GGAATAGAATGGAACCTGTCCCCTGTTGGCAGAGTTACCCCAAAGGAATGGAGGAATCAG
  TAA
  
  Clone variation with respect to NM_001866.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001866 unedited
  GTCGGATTTGTATACGACTTCTATAGGCGGCCGCGNAATTCGCACGAGGCTGAAGCGAAT
  TGGCACCAAAGCAGCAGCTGTATTGCCGCAGTTCTAGCTTCACCTTCACGATGTTTCCCT
  TGGTCAAAAGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAGGC
  AGAGCCACCAGAAACGTACACCTGATTTTCATGACAAATACGGTAATGCTGTATTAGCTA
  GTGGAGCCACTTTCTGTATTGTTACATGGACATATGTAGCAACACAAGTCGGAATAGAAT
  GGAACCTGTCCCCTGTTGGCAGAGTTACCCCAAAGGAATGGAGGAATCAGTAATCATCCC
  AGCTGGTGTAATAATGAATTGTTTAAAAAACAGCTCATAATTGATGCCAAATTAAAGCAC
  TGTGTACCCATTAAGATATGGCATTATTGAAGAAATAAAGTACATTTGAAACCTTCAAAA
  AAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGA
  ACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTT
  GCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGAC
  TAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGTTGGTATGGNANCCAGGGGCCAAG
  TTTGGAAAGACAACCTGTAGGGCCTGCCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGT
  GGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCA
  GCCTCCCGAGNTGTTTGGGATTCCCAGCATGCATGACCAGGCTCACTAATTTTTGGTCTT
  TTGGTAAGAACCGGGGTTCACCATAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001866 unedited
  GGCACGCATTTCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTGAAGGGTTCAAATGT
  ACTTTATTTCTTCAATAATGCCATATCTTAATGGGTACACAGTGCTTTAATTTGGCATCA
  ATTATGAGCTGTTTTTTAAACAATTCATTATTACACCAGCTGGGATGATTACTGATTCCT
  CCATTCCTTTGGGGTAACTCTGCCAACAGGGGACAGGTTCCATTCTATTCCGACTTGTGT
  TGCTACATATGTCCATGTAACAATACAGAAAGTGGCTCCACTAGCTAATACAGCATTACC
  GTATTTGTCATGAAAATCAGGTGTACGTTTCTGGTGGCTCTGCCTTGCCATTGTTTGCTG
  AATGCTTCGAACTTGGAGACGATTTATTGCGCTTTTGACCAAGGGAAACATCGTGAAGGT
  GAAGCTAGAACTGCGGCAATACAGCTGCTGCTTTGGTGCCAATTCGCTTCAGCCTCGTGC
  CGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGA
  GCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGNGCG
  GAGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTG
  ACGTCAATGGGGGTGGAGACTCGGAAATCCCCGTGAGTCATACCGCTATCCACGCCCATT
  GGNTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACCTANATGTACT
  GCCCAGTAGAAAGTCCCGTAAGGCATGTACTTGGCATATGCCCAGCGGCCATTTACGTCT
  TGACGNAATAGGGGGCGACCTGGCTATAACACTGAGTCTGCCAGGGGCAGTAACCTAAAA
  CTCACCATGACGCATGAAAGCCCTATGCGTACTGGGACAACTCTTTTGCCCAGGGCGGGT
  CGTTGCGCCACCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001866
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001866.2, NP_001857.1
• RefSeq Size: 456 bp
• RefSeq ORF: 243 bp
• Locus ID: 1349
• Cytogenetics: Xq21.1
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes subunit VIIb, which is highly similar to bovine COX VIIb protein and is found in all tissues. This gene may have several pseudogenes on chromosomes 1, 2, 20 and 22. [provided by RefSeq, Jun 2011]'