CDK7 (NM_001799) Human Untagged Clone

CAT#: SC119024

CDK7 (untagged)-Human cyclin-dependent kinase 7 (CDK7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK7
• Synonyms: CAK; CAK1; CDKN7; HCAK; MO15; p39MO15; STK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001799 edited
  ATGGCTCTGGACGTGAAGTCTCGGGCAAAGCGTTATGAGAAGCTGGACTTCCTTGGGGAG
  GGACAGTTTGCCACCGTTTACAAGGCCAGAGATAAGAATACCAACCAAATTGTCGCCATT
  AAGAAAATCAAACTTGGACATAGATCAGAAGCTAAAGATGGTATAAATAGAACCGCCTTA
  AGAGAGATAAAATTATTACAGGAGCTAAGTCATCCAAATATAATTGGTCTCCTTGATGCT
  TTTGGACATAAATCTAATATTAGCCTTGTCTTTGATTTTATGGAAACTGATCTAGAGGTT
  ATAATAAAGGATAATAGTCTTGTGCTGACACCATCACACATCAAAGCCTACATGTTGATG
  ACTCTTCAAGGATTAGAATATTTACATCAACATTGGATCCTACATAGGGATCTGAAACCA
  AACAACTTGTTGCTAGATGAAAATGGAGTTCTAAAACTGGCAGATTTTGGCCTGGCCAAA
  TCTTTTGGGAGCCCCAATAGAGCTTATACACATCAGGTTGTAACCAGGTGGTATCGGGCC
  CCCGAGTTACTATTTGGAGCTAGGATGTATGGTGTAGGTGTGGACATGTGGGCTGTTGGC
  TGTATATTAGCAGAGTTACTTCTAAGGGTTCCTTTTTTGCCAGGAGATTCAGACCTTGAT
  CAGCTAACAAGAATATTTGAAACTTTGGGCACACCAACTGAGGAACAGTGGCCGGACATG
  TGTAGTCTTCCAGATTATGTGACATTTAAGAGTTTCCCTGGAATACCTTTGCATCACATC
  TTCAGTGCAGCAGGAGACGACTTACTAGATCTCATACAAGGCTTATTCTTATTTAATCCA
  TGTGCTCGAATTACGGCCACACAGGCACTGAAAATGAAGTATTTCAGTAATCGGCCAGGG
  CCAACACCTGGATGTCAGCTGCCAAGACCAAACTGTCCAGTGGAAACCTTAAAGGAGCAA
  TCAAATCCAGCTTTGGCAATAAAAAGGAAAAGAACAGAGGCCTTAGAACAAGGAGGATTG
  CCCAAGAAACTAATTTTTTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001799 unedited
  NGTCGGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGGTAGCTTT
  AAATTCGTGTTGTCCTGGGAGCTCGCCCTTTTCGGCTGGAGTCGGGCTTTACGGCGCCGG
  ATGGCTCTGGACGTGAAGTCTCGGGCAAAGCGTTATGAGAAGCTGGACTTCCTTGGGGAG
  GGACAGTTTGCCACCGTTTACAAGGCCAGAGATAAGAATACCAACCAAATTGTCGCCATT
  AAGAAAATCAAACTTGGACATAGATCAGAAGCTAAAGATGGTATAAATAGAACCGCCTTA
  AGAGAGATAAAATTATTACAGGAGCTAAGTCATCCAAATATAATTGGTCTCCTTGATGCT
  TTTGGACATAAATCTAATATTAGCCTTGTCTTTGATTTTATGGAAACTGATCTAGAGGTT
  ATAATAAAGGATAATAGTCTTGTGCTGACACCATCACACATCAAAGCCTACATGTTGATG
  ACTCTTCAAGGATTAGAATATTTACATCAACATTGGATCCTACATAGGGATCTGAAACCA
  AACAACTTGTTGCTAGATGAAAATGGAGTTCTAAAACTGGCAGATTTTGGCCTGGCCAAA
  TCTTTTGGGAGCCCCAATAGAGCTTATACACATCAGGTTGTAACCAGGTGGTATCGGGCC
  CCCGAGTTACTATTTGGAGCTAGGATGTATGGTGTANGTGTGGACATGTGGGCTGTTGGC
  TGTATATTAGCAGAGTTACTTCTAAGGGTTCCTTTTTTGCCAGNAGATTCAGACCTTGAT
  CAGCTAACAAGAATATTTGAAACTTTGNGCACACCAACTGAGNAACAGTGGCCGGACATG
  TGTAGTCTTCCAGAATATGTGACATTTAAGAGTTTCCCTGGATACTTNGCATCACATCTC
  AGTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001799 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATTTTTATTTTAAAATACAT
  TTAATAAAAATAACCCATAGTTTTACATATTTTACATGTGTAGAATATTTACAAACTCAC
  TTCTACAGCATTTACTTAATGTTTACTATTTTTCCATTATTTGCCTTTTTGGCTATTTCC
  CTCAGTAGTAAAATGTTGTCCAGTGTTCTCTTTAAAAAATTAGTTTCTTGGGCAATCCTC
  CTTGTTCTAAGGCCTCTGTTCTTTTCCTTTTTATTGCCAAAGCTGGATTTGATTGCTCCT
  TTAAGGTTTCCACTGGACAGTTTGGTCTTGGCAGCTGACATCCAGGTGTTGGCCCTGGCC
  GATTACTGAAATACTTCATTTTCAGTGCCTGTGTGGCCGTAATTCGAGCACATGGATTAA
  ATAAGAATAAGCCTTGTATGAGATCTAGTAAGTCGTCTCCTGCTGCACTGAAGATGTGAT
  GCAAAGGTATTCCAGGGAAACTCTTAAATGTCACATAATCTGGAAGACTACACATGTCCG
  GCCACTGTTCCTCAGTTGGTGTGCCCAAAGTTTCAAATATTCTTGTTAGCTGATCAAGGT
  CTGAATCTCCTGGCAAAAAAGGAACCCTTAGAAGTAACTCTGCTAATATACAGCCAACAG
  CCCACATGTCCACACCTACACCATACATCCTAGCTCCAAATAGTAACTCGGNGGCCCGAT
  ACCACCTGGTTACAACCTGATGTGTATAAGCTCTATTGGGGCTCCCAAAAGAATTGGCCA
  GGCCAAAATCTGCCAGTTTTAGAACTCCATTCTCATCTAGCAACCCAGTGTTTGGNTTCA
  GATCCCTATGTAGATCCAAGTTGATGTAAATATCTAATCCTTGAGAGTCTCACCTGTTAG
  CTTGAGGGTGATGGGTCACCACAGAAATATCCTTATTAAACCCTTAACAGTTCCATAAAA
  CAAGCAAGCTAATTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001799
• Insert Size: 1280 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001799.2, NP_001790.1
• RefSeq Size: 1427 bp
• RefSeq ORF: 1041 bp
• Locus ID: 1022
• Cytogenetics: 5q13.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, Nucleotide excision repair
• Gene Summary: 'The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008]'