ATP6V1B2 (NM_001693) Human Untagged Clone

CAT#: SC119083

ATP6V1B2 (untagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)


  "NM_001693" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ATP6V1B2
Synonyms ATP6B1B2; ATP6B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001693, the custom clone sequence may differ by one or more nucleotides


ATGGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCACCGGTGGGC
CGGCGGTGGGAGCTCGGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTCCCAGCCTCGCCTCACATA
CAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGATCATGTTAAGTTTCCCAGGTATGCTGAA
ATTGTCCATTTGACCTTACCGGATGGCACAAAGAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGG
CAGTAGTTCAGGTATTTGAAGGGACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGA
TATTCTCCGAACACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGAC
AGAGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCAATGTCGAA
TCTACCCAGAGGAAATGATTCAGACTGGCATTTCGGCCATCGATGGGATGAACAGTATTGCTAGGGGGCA
GAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGAGATTGCAGCTCAGATCTGTCGCCAGGCT
GGTTTGGTAAAGAAATCCAAAGATGTAGTAGACTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTA
TGGGTGTAAACATGGAAACTGCCCGGTTCTTCAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT
CTGCCTCTTTTTGAACTTGGCTAATGACCCAACCATTGAGCGAATTATCACTCCTCGCCTGGCTCTAACC
ACAGCTGAATTTCTGGCGTACCAATGTGAGAAACATGTATTGGTTATTCTAACAGACATGAGTTCTTATG
CTGAAGCACTTCGAGAGGTTTCAGCAGCCAGGGAAGAGGTACCTGGTCGACGAGGTTTTCCAGGTTACAT
GTATACAGATTTAGCCACGATATATGAACGCGCTGGGCGAGTGGAAGGGAGAAACGGCTCGATTACTCAA
ATCCCTATTCTAACCATGCCTAATGATGATATCACTCACCCCATCCCAGACTTGACTGGCTACATTACAG
AGGGGCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTATCCACCTATCAATGTGCTGCCCTC
ACTATCACGGTTAATGAAGTCTGCTATTGGAGAAGGGATGACCAGGAAGGATCATGCCGATGTATCTAAC
CAGCTATATGCGTGCTATGCTATTGGAAAGGATGTGCAAGCCATGAAAGCTGTCGTTGGAGAAGAAGCCC
TTACCTCAGATGATCTTCTCTACTTGGAATTTCTGCAGAAGTTTGAGAGGAACTTCATTGCTCAGGGTCC
TTACGAAAATCGCACTGTCTTTGAGACTTTGGACATTGGCTGGCAGCTACTCCGAATCTTCCCCAAAGAA
ATGCTGAAGAGAATCCCTCAGAGCACCCTCAGCGAATTTTACCCTCGAGACTCTGCAAAGCATTAG


>OriGene 5' read for NM_001693 unedited
TTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGACAGAGGAGACAAGAT
GGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCAC
CGGTGGGCCGGCGGTGGGAGCTCGGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTC
CCAGCCTCGCCTCACATACAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGA
TCATGTTAAGTTTCCCAGGTATGCTGAAATTGTCCATTTGACCTTACCGGATGGCACAAA
GAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGGCAGTAGTTCAGGTATTTGAAGG
GACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGATATTCTCCGAAC
ACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGACAG
AGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCA
ATGTCGAATCTACCCAGAGGAAATGATTCAGACTGGCATTTCGGCCATCGATGGGATGAA
CAGTATTGCTAGGGGGCAGAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGA
GATTGCAGCTCAGATCTGTCGCCAGGCTGGTTTGGTAAAGAAATCCAAAGATGTAGTAGA
CTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTATGGGGTGTAACATGGNNAACTG
NCCNGTTCTTCANNATCTGACTTGAAGAAAATGGCTCATGGGACAATGTCTGCCTCTTTN
TGAAACTGGGCTATGACCCACCATTGAGCGAATATCACTNCTCGNCTGGCTCTACCCAGT
A
>OriGene 3' read for NM_001693 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTAATTTAACATTTATTT
TCATTTTATATCCAGATGTCACATTTTCTACATTAAAATATAATCATGCTGACTCCCCCA
ACCCCCAGCCCTCAGAGAAAAAAGTGAAGAACTTTACACAACATGAAAGATGGAATGCAC
GGGGTAGGGAAGTTGCTATTGCACCCAAGTAACAAAGAACACAGAGAGACCAACAGGACG
CATGGCAGAACTGCATAGAAGATCGCTCAGGGCTCACGTCAGGAAGGCGCAGGAATTCAG
AAAACTCTAAAGCTTGGAAATGCTATCGTTTGTGCCACAAAGATTACCCTGAAAAGGTAG
AGAGAGGTCAAAAAGCAGAACCAAATTGGGGATTTTCAAAGGCTGCTCTCTAACAGAGTT
TAAAATATCCGTAAACATAAGTGCTTCTGCTCCCTCCCCTTGCCTTCAGCATCTGGTAAA
CACTTTAGAAACTTAAACCACTGTTAGCATGTCAGAATGTAGAAAGGCTGTAGGTTTTTC
TTTCTTCTCATCTTGTACCCCAAACAACAACTCTACTGGCAATTGTATTCATGTGACTCC
TCTGCTTGGAAACCAGTAAGGATCACCAAGACAAGGGGTCTCACTGCTACCAGACAGATA
CCAGCTGTGCAAAAAGTTGCCAGATTCTGTGTATTATTTGGAGAGGGAGAGCTGTAAGAC
ACAGGAATCCAATACGTAAGTACTAAAGAGAAAAAGAAACCCCTTTCCCTCATCTATTCC
TTATATAGGAAAGAGCCAAAAGACCGTCAAACCTTCTGCTTTGAGCAGAACACACGCCAC
AATACAGGCTACCACCAACCGAAGCCACCCTCAGAAAAACCTCTTGGCCTTCCAAAGAAA
CTTCCTTAACTGGATCTGCCCTATGGAAAACAAGCTTTTATTTAAAACTGACTTT
Restriction Sites NotI-NotI     
ACCN NM_001693
Insert Size 2940 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001693.2, NP_001684.2
RefSeq Size 3054 bp
RefSeq ORF 3054 bp
Locus ID 526
Cytogenetics 8p21.3
Domains ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N
Protein Families Druggable Genome
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]'

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