ATP6V1B2 (NM_001693) Human Untagged Clone

CAT#: SC119083

ATP6V1B2 (untagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V1B2
• Synonyms: ATP6B1B2; ATP6B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001693, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCACCGGTGGGC
  CGGCGGTGGGAGCTCGGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTCCCAGCCTCGCCTCACATA
  CAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGATCATGTTAAGTTTCCCAGGTATGCTGAA
  ATTGTCCATTTGACCTTACCGGATGGCACAAAGAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGG
  CAGTAGTTCAGGTATTTGAAGGGACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGA
  TATTCTCCGAACACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGAC
  AGAGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCAATGTCGAA
  TCTACCCAGAGGAAATGATTCAGACTGGCATTTCGGCCATCGATGGGATGAACAGTATTGCTAGGGGGCA
  GAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGAGATTGCAGCTCAGATCTGTCGCCAGGCT
  GGTTTGGTAAAGAAATCCAAAGATGTAGTAGACTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTA
  TGGGTGTAAACATGGAAACTGCCCGGTTCTTCAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT
  CTGCCTCTTTTTGAACTTGGCTAATGACCCAACCATTGAGCGAATTATCACTCCTCGCCTGGCTCTAACC
  ACAGCTGAATTTCTGGCGTACCAATGTGAGAAACATGTATTGGTTATTCTAACAGACATGAGTTCTTATG
  CTGAAGCACTTCGAGAGGTTTCAGCAGCCAGGGAAGAGGTACCTGGTCGACGAGGTTTTCCAGGTTACAT
  GTATACAGATTTAGCCACGATATATGAACGCGCTGGGCGAGTGGAAGGGAGAAACGGCTCGATTACTCAA
  ATCCCTATTCTAACCATGCCTAATGATGATATCACTCACCCCATCCCAGACTTGACTGGCTACATTACAG
  AGGGGCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTATCCACCTATCAATGTGCTGCCCTC
  ACTATCACGGTTAATGAAGTCTGCTATTGGAGAAGGGATGACCAGGAAGGATCATGCCGATGTATCTAAC
  CAGCTATATGCGTGCTATGCTATTGGAAAGGATGTGCAAGCCATGAAAGCTGTCGTTGGAGAAGAAGCCC
  TTACCTCAGATGATCTTCTCTACTTGGAATTTCTGCAGAAGTTTGAGAGGAACTTCATTGCTCAGGGTCC
  TTACGAAAATCGCACTGTCTTTGAGACTTTGGACATTGGCTGGCAGCTACTCCGAATCTTCCCCAAAGAA
  ATGCTGAAGAGAATCCCTCAGAGCACCCTCAGCGAATTTTACCCTCGAGACTCTGCAAAGCATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001693 unedited
  TTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGACAGAGGAGACAAGAT
  GGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCAC
  CGGTGGGCCGGCGGTGGGAGCTCGGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTC
  CCAGCCTCGCCTCACATACAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGA
  TCATGTTAAGTTTCCCAGGTATGCTGAAATTGTCCATTTGACCTTACCGGATGGCACAAA
  GAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGGCAGTAGTTCAGGTATTTGAAGG
  GACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGATATTCTCCGAAC
  ACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGACAG
  AGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCA
  ATGTCGAATCTACCCAGAGGAAATGATTCAGACTGGCATTTCGGCCATCGATGGGATGAA
  CAGTATTGCTAGGGGGCAGAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGA
  GATTGCAGCTCAGATCTGTCGCCAGGCTGGTTTGGTAAAGAAATCCAAAGATGTAGTAGA
  CTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTATGGGGTGTAACATGGNNAACTG
  NCCNGTTCTTCANNATCTGACTTGAAGAAAATGGCTCATGGGACAATGTCTGCCTCTTTN
  TGAAACTGGGCTATGACCCACCATTGAGCGAATATCACTNCTCGNCTGGCTCTACCCAGT
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001693 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTAATTTAACATTTATTT
  TCATTTTATATCCAGATGTCACATTTTCTACATTAAAATATAATCATGCTGACTCCCCCA
  ACCCCCAGCCCTCAGAGAAAAAAGTGAAGAACTTTACACAACATGAAAGATGGAATGCAC
  GGGGTAGGGAAGTTGCTATTGCACCCAAGTAACAAAGAACACAGAGAGACCAACAGGACG
  CATGGCAGAACTGCATAGAAGATCGCTCAGGGCTCACGTCAGGAAGGCGCAGGAATTCAG
  AAAACTCTAAAGCTTGGAAATGCTATCGTTTGTGCCACAAAGATTACCCTGAAAAGGTAG
  AGAGAGGTCAAAAAGCAGAACCAAATTGGGGATTTTCAAAGGCTGCTCTCTAACAGAGTT
  TAAAATATCCGTAAACATAAGTGCTTCTGCTCCCTCCCCTTGCCTTCAGCATCTGGTAAA
  CACTTTAGAAACTTAAACCACTGTTAGCATGTCAGAATGTAGAAAGGCTGTAGGTTTTTC
  TTTCTTCTCATCTTGTACCCCAAACAACAACTCTACTGGCAATTGTATTCATGTGACTCC
  TCTGCTTGGAAACCAGTAAGGATCACCAAGACAAGGGGTCTCACTGCTACCAGACAGATA
  CCAGCTGTGCAAAAAGTTGCCAGATTCTGTGTATTATTTGGAGAGGGAGAGCTGTAAGAC
  ACAGGAATCCAATACGTAAGTACTAAAGAGAAAAAGAAACCCCTTTCCCTCATCTATTCC
  TTATATAGGAAAGAGCCAAAAGACCGTCAAACCTTCTGCTTTGAGCAGAACACACGCCAC
  AATACAGGCTACCACCAACCGAAGCCACCCTCAGAAAAACCTCTTGGCCTTCCAAAGAAA
  CTTCCTTAACTGGATCTGCCCTATGGAAAACAAGCTTTTATTTAAAACTGACTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001693
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001693.2, NP_001684.2
• RefSeq Size: 3054 bp
• RefSeq ORF: 3054 bp
• Locus ID: 526
• Cytogenetics: 8p21.3
• Domains: ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N
• Protein Families: Druggable Genome
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]'