ACADL (NM_001608) Human Untagged Clone

CAT#: SC119148

ACADL (untagged)-Human acyl-CoA dehydrogenase, long chain (ACADL), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACADL
• Synonyms: ACAD4; LCAD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119148 sequence for NM_001608 edited (data generated by NextGen Sequencing)
  ATGGCCGCACGCCTTCTCCGAGGGTCCCTACGCGTCCTGGGCGGCCACCGTGCGCCGCGC
  CAGCTGCCCGCCGCGCGATGTTCTCATTCCGGAGGGGAAGAACGTCTAGAAACTCCTTCT
  GCTAAAAAATTAACAGATATAGGAATTCGAAGAATCTTTTCTCCAGAGCATGACATTTTC
  CGGAAAAGTGTAAGGAAGTTTTTCCAAGAAGAAGTGATTCCTCATCACTCAGAATGGGAG
  AAAGCTGGAGAAGTAAGTAGGGAGGTTTGGGAAAAAGCTGGAAAACAAGGACTGCTTGGT
  GTCAATATTGCAGAGCATCTTGGTGGAATTGGAGGGGATCTGTACTCCGCAGCTATTGTC
  TGGGAGGAGCAAGCTTATTCAAATTGTTCAGGCCCAGGTTTTAGTATTCATTCAGGTATT
  GTCATGTCCTATATTACAAACCATGGCTCAGAAGAACAGATTAAGCACTTTATTCCCCAG
  ATGACTGCAGGCAAATGTATTGGTGCAATAGCAATGACAGAGCCTGGAGCTGGAAGTGAC
  TTACAGGGAATAAAAACAAATGCTAAAAAGGATGGAAGTGACTGGATTCTCAATGGAAGC
  MAGGTGTTCATCAGTAATGGGTCATTAAGTGATGTTGTGATTGTAGTTGCGGTCACAAAT
  CATGAAGCTCCCTCCCCTGCCCATGGTATTAGCCTTTTTCTGGTGGAAAATGGAATGAAA
  GGATTTATCAAGGGACGAAAGCTACATAAAATGGGATTAAAAGCCCAGGATACCGCAGAA
  CTATTCTTTGAAGATATACGGTTGCCAGCTAGTGCCCTACTTGGAGAAGAGAATAAAGGC
  TTCTATTACATCATGAAAGAGCTTCCACAGGAAAGGCTGTTAATTGCTGATGTGGCAATT
  TCAGCTAGTGAATTCATGTTTGAAGAAACCAGGAACTATGTTAAACAAAGAAAAGCTTTT
  GGCAAAACAGTTGCTCACCTACAGACAGTGCAACATAAATTAGCAGAATTAAAAACACAT
  ATATGTGTAACCCGAGCATTTGTGGACAACTGTCTCCAGCTGCATGAAGCGAAACGTTTG
  GACTCCGCCACTGCTTGCATGGCGAAATATTGGGCATCTGAGTTACAAAATAGTGTAGCT
  TACGACTGTGTACAGCTCCATGGAGGTTGGGGATACATGTGGGAGTACCCAATTGCAAAA
  GCTTATGTGGATGCCAGAGTTCAGCCAATCTATGGTGGTACAAATGAAATAATGAAGGAG
  CTGATTGCAAGAGAGATTGTCTTTGACAAGTAG
  
  Clone variation with respect to NM_001608.3
  601 a=>m
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001608 unedited
  TTTCAGCATTTGTATACGATTCCTATAGGCGGCNCGCGATTCGGCACGAGGTGGCGTGGT
  TGGGGCTCCGGAGGGCGCGCGCGAGCGCTTTTTTGGGAGGACACCACAGGTGGACGCCTC
  AGCTGATCGTCCTCCCTCCCGGGGACCCTGCCCCGAGTCGCCGAGTAGCCGCAGAGTCGC
  CTCCGTCGCCCCGCCGCCCCTGTGTTTCGGACATGGCCGCACGCCTTCTCCGAGGGTCCC
  TACGCGTCCTGGGCGGCCACCGTGCGCCGCGCCAGCTGCCCGCCGCGCGATGTTCTCATT
  CCGGAGGGGAAGAACGTCTAGAAACTCCTTCTGCTAAAAAATTAACAGATATAGGAATTC
  GAAGAATCTTTTCTCCAGAGCATGACATTTTCCGGAAAAGTGTAAGGAAGTTTTTCCAAG
  AAGAAGTGATTCCTCATCACTCAGAATGGGAGAAAGCTGGAGAAGTAAGTAGGGAGGTTT
  GGGAAAAAGCTGGAAAACAAGGACTGCTTGGTGTCAATATTGCAGAGCATCTTGGTGGAA
  TTGGAGGGGATCTGTACTCCGCAGCTATTGTCTGGGAGGAGCAGTAAGTATGGAGACTTT
  TAAGGTTGAGTCTTGTTTTGTAAACACACCACTCACCTTTCTTGAATGACCCAACAAACA
  AACCAACAAACAAATGTTTTCTAATCTTGGAAAGATGAAATTCAGTGTAAATGTAAAAGA
  TATACCGCTCATGCACTCTTGACATTTTTTGAATGGGGGATATAAGAAAATCCTTTTAAG
  TTGCTGAAATAGGTGCTATTTGAAAATTAAGAGATAGTTTTTTTTTTTCCTGCCATGCCT
  ATGANTACTTTTCCCCTCTAAAAAGAATTTTGAAAGACACCGCCGAGGAGAAAATTATTC
  CCTCAAAATTTTACAAATTTAATTTCCGGACAATAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001608
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001608.2, NP_001599.1
• RefSeq Size: 2497 bp
• RefSeq ORF: 1293 bp
• Locus ID: 33
• Cytogenetics: 2q34
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq, Jul 2008]'