GSTA4 (NM_001512) Human Untagged Clone

CAT#: SC119179

GSTA4 (untagged)-Human glutathione S-transferase alpha 4 (GSTA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSTA4
• Synonyms: GSTA4-4; GTA4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119179 sequence for NM_001512 edited (data generated by NextGen Sequencing)
  ATGGCAGCAAGGCCCAAGCTCCACTATCCCAACGGAAGAGGCCGGATGGAGTCCGTGAGA
  TGGGTTTTAGCTGCCGCCGGAGTCGAGTTTGATGAAGAATTTCTGGAAACAAAAGAACAG
  TTGTACAAGTTGCAGGATGGTAACCACCTGCTGTTCCAACAAGTGCCCATGGTTGAAATT
  GACGGGATGAAGTTGGTACAGACCCGAAGCATTCTCCACTACATAGCAGACAAGCACAAT
  CTCTTTGGCAAGAACCTCAAGGAGAGAACCCTGATTGACATGTACGTGGAGGGGACACTG
  GATCTGCTGGAACTGCTTATCATGCATCCTTTCTTAAAACCAGATGATCAGCAAAAGGAA
  GTGGTTAACATGGCCCAGAAGGCTATAATTAGATACTTTCCTGTGTTTGAAAAGATTTTA
  AGGGGTCACGGACAAAGCTTTCTTGTTGGTAATCAGCTGAGCCTTGCAGATGTGATTTTA
  CTCCAAACCATTTTAGCTCTAGAAGAGAAAATTCCTAATATCCTGTCTGCATTTCCTTTC
  CTCCAGGAATACACAGTGAAACTAAGTAATATCCCTACAATTAAGAGATTCCTTGAACCT
  GGCAGCAAGAAGAAGCCTCCCCCTGATGAAATTTATGTGAGAACCGTCTACAACATCTTT
  AGGCCATAA
  
  Clone variation with respect to NM_001512.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001512 unedited
  CGGTGTGTCACCTATATGTTNGTCAATCCACGCACGTTCACTTATTCAGGGNCGGNCCCG
  CGCCAATTNCGGNCACGCAGGGCTTCCGNAGTGGTACTCCAGNAAAGCCTTGAAAAGCTA
  TCATGGCAGCAAGGCCCAAGCTCCACTATCCCAACGGAAGAGGCCGGATGGAGTCCGTGA
  GATGGGTTTTAGCTGCCGCCGGAGTCGAGTTTGATGAAGAATTTCTGGAAACAAAAGAAC
  AGTTGTACAAGTTGCAGGATGGTAACCACCTGCTGTTCCAACAAGTGCCCATGGTTGAAA
  TTGACGGGATGAAGTTGGTACAGACCCGAAGCATTCTCCACTACATAGCAGACAAGCACA
  ATCTCTTTGGCAAGAACCTCAAGGAGAGAACCCTGATTGACATGTACGTGGAGGGGACAC
  TGGATCTGCTGGAACTGCTTATCATGCATCCTTTCTTAAAACCAGATGATCAGCAAAAGG
  AAGTGGTTAACATGGCCCAGAAGGCTATAATTAGATACTTTCCTGTGTTTGAAAAGATTT
  TAAGGGGTCACGGACAAAGCTTTCTTGTTGGTAATCAGCTGAGCCTTGCAGATGTGATTT
  TACTCCAAACCATTTTAGCTCTAGAAGAGAAAATTCCTAGTATCCTGTCTGCATTTCCTT
  TCCTCCAGGAGTACACAGTGAAACTAAGTAATATCCCTCCATTAAGAGATTCCTTTGACC
  TGCCAGCCAGAAGAGCCTCCCCCTGATGAAATTATGTGAGACCGTTTACAACATCTTTAG
  GCCATAAAACAACACATCCATGTGTGAGTGACCGGGTGTTCTTAGAGATGGTATTGCTTC
  CGTCATGTTTTATGGGATCCCAGCTCTGCCATGGGGCTACTATGTTATAAAGTGGGGCCT
  AAGTTGGGCCTTTTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001512 unedited
  ACTTCCAGGGCCGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC
  AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGACCCAAAAATTCTTTATTTGG
  GCTAAAACGGATGGCATTCATTACCCCCATTTTATTTATTACCGCCCAAGAGGAAAGTCT
  TTTTTTAAACCTTCGGGGGTTTTTAAACCCTAAAAATTAGGCCAAAACTGGGGGGGATAA
  GGATAAAAAAACCGGATAGGGAGACCCAAAAGCCCCTCAGGAAAGTAAAAAGACCCTCGG
  GAAAAAACAAAAAACCCCGCCCATTCTTATTATGAGGATGAACTGGCTACAGGACATGAG
  TACCCCAATCAAATCTAACTTAAATCCCCCCCCCTCCAAAAAAGTTTTCCAATAAAAACC
  CCCTAACAATTATTTACGGGACAAAAAAGGGTGATTTTTCTAAAAAAAATGATCTCGTTG
  ACCCAAAAAACCCAACTTAGGCCCCAACTTAATACATAAATAGCCCCCTGACAGAGCTGG
  GACCCATTAAAACATGACTGGAAACAATCCCATCTTTAGGAACACACTGGCACTCCACAT
  GGGATGTGTTGTTTTATGGCCTAAAAATGTTGGAAACGGTTCTCACATAAATTTCATAGG
  GGGAGGCTTCTTCTTGCTGCCAGTTCAAGGAATCTTTTAATGGAGGGAATTACTTANTTT
  CCTGGGNATTCCTGGGGAAAGGAACGCCAACCGGATTTAGAATTTCCTCTTCAAGCAAAA
  GGGTTGAGTAAACCCATGGGAGGCTCAGCTGATCCAACGAAAGCTTGCCGGGACCCTTAA
  ATCTTTAAACAAGGAAGGTCTATTTAGCCTTGGGGCAGTTACCCCTCCTTTGGGGAAATT
  GGTTTAAAGGGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001512
• Insert Size: 1310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001512.2, NP_001503.1
• RefSeq Size: 1317 bp
• RefSeq ORF: 669 bp
• Locus ID: 2941
• Cytogenetics: 6p12.2
• Domains: GST_N, GST_C
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq, Jul 2008]'