AKR1C1 (NM_001353) Human Untagged Clone

CAT#: SC119276

AKR1C1 (untagged)-Human aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) (AKR1C1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKR1C1
• Synonyms: 2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1; DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001353 edited
  GAGAAAGCCATGTAACCATGAAGGCAGAGATTGAAGTGATTCAACTATAAGCCAAGGCAT
  GCCAACTGCTACTGGGAGCCACCAAAAGCTAGTCAAAGGCAAGGGAACCATCTTCCCGAG
  AGCTTTCATGGGGAGCATGGCCCTATAAACAACTGGATTTTTGACTCAAGTCTCCAGAAC
  TGGAAGAGAATACAATCCTCTTTTGTTGTTCTAAACCATCCAATTTGCACAAAAACGTGT
  TTTGAACCTACTCTAACCAACTGTCAAAAGGCTGTGTTAAGTGATTCCCTGACAAGAACC
  GGGATTTAGCTGAACTAAGAAGCAAAAATTCTGCATCATTTTGGTGGCGTGTACAGGGAC
  ATGAGGAAGGAACCTTGGCCATTCTGATGTTTAACCAAGAGTTGAGCTGAGTGGCTCTGA
  CCAGCGTGGAAGATGAGAGCAGCCTGAACTTACACTGTGAAAATGCCCTGGAGAAATGCA
  GAGATGCAGGTTTAATGAAGTCCATCAGGGTGTCCAGTTTCAATCACTAACTGTTGGAAC
  TGATCCTCAGACAAGCCAGGACTCAAGTACAAGCCCATCTACAACCAGATGAGCCCTTAG
  GGCCACTGGGGCCATTTCTGACTTTGTGCTCTTCACACACTTCCTTACATTAGAATAATG
  TAAGAAACGGTTGAACTGGATGCAATTTTTATCACAGCTTGTGTAAGACTGCCTCTGTCC
  CTCCTCTCACATGCCATTGGTTAACCAGCAGACAGTGTGCTCAGGGGCGTTGCCAGCTCA
  TTGCTCTTATAGCCTGTGAGGGAGGAAGAAAGAAACATTTGCCAGCCAGGCTAGTGACAG
  AAATGGATTCGAAATATCAGTGTGTGAAGCTGAATGATGGTCACTTCATGCCTGTCCTGG
  GATTTGGCACCTATGCGCCTGCAGAGGTTCCTAAAAGTAAAGCTTTAGAGGCCACCAAAT
  TGGCAATTGAAGCTGGCTTCCGCCATATTGATTCTGCTCATTTATACAATAATGAGGAGC
  AGGTTGGACTGGCCATCCGAAGCAAGATTGCAGATGGCAGTGTGAAGAGAGAAGACATAT
  TCTACACTTCAAAGCTTTGGTGCAATTCCCATCGACCAGAGTTGGTCCGACCAGCCTTGG
  AAAGGTCACTGAAAAATCTTCAATTGGATTATGTTGACCTCTACCTTATTCATTTTCCAG
  TGTCTGTAAAGCCAGGTGAGGAAGTGATCCCAAAAGATGAAAATGGAAAAATACTATTTG
  ACACAGTGGATCTCTGTGCCACGTGGGAGGCCGTGGAGAAGTGTAAAGATGCAGGATTGG
  CCAAGTCCATCGGGGTGTCCAACTTCAACCGCAGGCAGCTGGAGATGATCCTCAACAAGC
  CAGGGCTCAAGTACAAGCCTGTCTGCAACCAGGTGGAATGTCATCCTTACTTCAACCAGA
  GAAAACTGCTGGATTTCTGCAAGTCAAAAGACATTGTTCTGGTTGCCTATAGTGCTCTGG
  GATCCCACCGAGAAGAACCATGGGTGGACCCGAACTCCCCGGTGCTCTTGGAGGACCCAG
  TCCTTTGTGCCTTGGCAAAAAAGCACAAGCGAACCCCAGCCCTGATTGCCCTGCGCTACC
  AGCTACAGCGTGGGGTTGTGGTCCTGGCCAAGAGCTACAATGAGCAGCGCATCAGACAGA
  ACGTGCAGGTGTTTGAATTCCAGTTGACTTCAGAGGAGATGAAAGCCATAGATGGCCTAA
  ACAGAAATGTGCGATATTTGACCCTTGATATTTTTGCTGGCCCCCCTAATTATCCATTTT
  CTGATGAATATTAACATGGAGGGCATTGCATGAGGTCTACCAGAAGGCCCTGCGTGTGGA
  TGGTGACACAGAGGATGGCTCTATGCTGGTGACTGGACACATCGCCTCTGGTTAAATCTC
  TCCTGCTTGGTGATTTCAGCAAGCTACAGCAAAGCCCATTGGCCAGAAAGGAAAGACAAT
  AATTTTGTTTTTTCATTTTGAAAAAATTAAATGCTCTCTCCTAAAAAAAAAAAAAAAAAA
  AAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001353
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001353.5, NP_001344.2
• RefSeq Size: 1384 bp
• RefSeq ORF: 972 bp
• Locus ID: 1645
• Cytogenetics: 10p15.1
• Domains: aldo_ket_red
• Protein Families: Druggable Genome
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]'