DDT (NM_001355) Human Untagged Clone

CAT#: SC119278

DDT (untagged)-Human D-dopachrome tautomerase (DDT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDT
• Synonyms: D-DT; DDCT; MIF-2; MIF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119278 sequence for NM_001355 edited (data generated by NextGen Sequencing)
  ATGCCGTTCCTGGAGCTGGACACGAATTTGCCCGCCAACCGAGTCCCCGCGGGGCTGGAG
  AAACGACTCTGCGCCGCCGCTGCCTCCATCCTGGGCAAACCTGCGGACCGCGTGAACGTG
  ACGGTACGGCCGGGCCTGGCCATGGCGCTGAGCGGGTCCACCGAGCCCTGCGCGCAGCTG
  TCCATCTCCTCCATCGGCGTAGTGGGCACCGCCGAGGACAACCGCAGCCACAGCGCCCAC
  TTCTTTGAGTTTCTCACCAAGGAGCTAGCCCTGGGCCAGGACCGGATACTTATCCGCTTT
  TTCCCCTTGGAGTCCTGGCAGATTGGCAAGATAGGGACGGTCATGACTTTTTTATGA
  
  Clone variation with respect to NM_001355.3
  45 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001355 unedited
  TGTCAAAATTGTATACGACTCATATAGGCGGCNCGCGAATCGGCACGAGGCCGTTCCTCT
  GCCCGCCATGCCGTTCCTGGAGCTGGACACGAATTTGCCCGCCAACCGAGTCCCCGCGGG
  GCTGGAGAAACGACTCTGCGCCGCCGCTGCCTCCATCCTGGGCAAACCTGCGGACCGCGT
  GAACGTGACGGTACGGCCGGGCCTGGCCATGGCGCTGAGCGGGTCCACCGAGCCCTGCGC
  GCAGCTGTCCATCTCCTCCATCGGCGTAGTGGGCACCGCCGAGGACAACCGCAGCCACAG
  CGCCCACTTCTTTGAGTTTCTCACCAAGGAGCTAGCCCTGGGCCAGGACCGGATACTTAT
  CCGCTTTTTCCCCTTGGAGTCCTGGCAGATTGGCAAGATAGGGACGGTCATGACTTTTTT
  ATGATTGGGCACGGAGGGATCCAGGGCATCTGTGAACTGGCTGCTTCTTCCAGAGAGATC
  TCTTGGCAGAGTGAGGGCCTGGAGATAACCAGCTTTGGATTATCCCGCATGCAACATTCC
  TGTGATCACATAATCCTCTTCTTCATCCTCATATGAAATAAATGAAGAGAGCTTCCTCAT
  TCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTG
  AACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGT
  TGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGA
  CTAGGTGTCCTTCTTAATATTATGGGGTGAGGGGGNGTGGGNGATTGNGAACCAGGGGGC
  AAGNTNGGNAANNNACACCTGTAGGCCTGCCGGGGTCTATTGGGAACCCAGCTGGANTGC
  AGTGGCACAATCTTGGCTCACTGGCATCTCCGCCTNCTGGGGTTCAAGCGATNCTCCTGN
  CTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001355 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAATGAGGAAGCTCTCTTCATTTAT
  TTCATATGAGGATGAAGAAGAGGATTATGTGATCACAGGAATGTTGCATGCGGGATAATC
  CAAAGCTGGTTATCTCCAGGCCCTCACTCTGCCAAGAGATCTCTCTGGAAGAAGCAGCCA
  GTTCACAGATGCCCTGGATCCCTCCGTGCCCAATCATAAAAAAGTCATGACCGTCCCTAT
  CTTGCCAATCTGCCAGGACTCCAAGGGGAAAAAGCGGATAAGTATCCGGTCCTGGCCCAG
  GGCTAGCTCCTTGGTGAGAAACTCAAAGAAGTGGGCGCTGTGGCTGCGGTTGTCCTCGGC
  GGTGCCCACTACGCCGATGGAGGAGATGGACAGCTGCGCGCAGGGCTCGGTGGACCCGCT
  CAGCGCCATGGCCAGGCCCGGCCGTACCGTCACGTTCACGCGGTCCGCAGGTTTGCCCAG
  GATGGAGGCAGCGGCGGCGCAAAGTCGTTTCTCCAGCCCCGCGGGGACTCGGTTGGCGGG
  CAAATTCGTGTCCAGCTCCAGGAACGGCATGGCGGGCAGAGGAACGGCCTCGTGCCGAAT
  TCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCT
  GCTTATATAGACCTCCCACCGTAACGCCTACCGCCCATTNGCGTNACCGGGGCGGGGTTA
  TTACGACACTCTGGAAGTCCCCGTGATTCTGGTGCCCAACCAACTCCCATTGCCGTCATG
  GGGCGGAGACTTGGAAATCCCCGTGAGGCAAACGCGTATCCCCCCCATCGGCGCACCGCC
  AAACCGCATACCATGGGAATAGCGATGACTACACCGTCAATGGACCGCCCAATGGGAAAG
  CCTGCAAGGCAGTCCTGGGGCCACGGCCGGCCGGCCCTCACCGTTATTGCGCCATAGGGG
  GGGCCCTGCACAGAACCCTGCGGCCGGCTCGGGCCGTCCCCCACACTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001355
• Insert Size: 640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001355.2, NP_001346.1
• RefSeq Size: 666 bp
• RefSeq ORF: 357 bp
• Locus ID: 1652
• Cytogenetics: 22q11.23
• Gene Summary: 'D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.