DGKA (NM_001345) Human Untagged Clone

CAT#: SC119317

DGKA (untagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3


  "NM_001345" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DGKA
Synonyms DAGK; DAGK1; DGK-alpha
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119317 sequence for NM_001345 edited (data generated by NextGen Sequencing)
ATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATACATG
GAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGATGGCT
AAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAATCTAT
CTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGAGACT
GGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGTGGTGTGTCTCAATGATGTTTCC
TGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCTTCAAG
CTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTATCCTA
CAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGATTCTT
CAGGAGATGATGAAAGAGATTGACTATGATGGCAGTGGCTCTGTCTCTCAAGCTGAGTGG
GTCCGGGCTGGGGCCACCACCGTGCCACTGCTAGTGCTGCTGGGTCTGGAGATGACTCTG
AAGGACGACGGACAGCACATGTGGAGGCCCAAGAGGTTCCCCAGACCAGTCTACTGCAAT
CTGTGCGAGTCAAGCATTGGTCTTGGCAAACAGGGACTGAGCTGTAACCTCTGTAAGTAC
ACTGTTCACGACCAGTGTGCCATGAAAGCCCTGCCTTGTGAAGTCAGCACCTATGCCAAG
TCTCGGAAGGACATTGGTGTCCAATCACATGTGTGGGTGCGAGGAGGCTGTGAGTCCGGG
CGCTGCGACCGCTGTCAGAAAAAGATCCGGATCTACCACAGTCTGACCGGGCTGCATTGT
GTATGGTGCCACCTAGAGATCCACGATGACTGCCTGCAAGCGGTGGGCCATGAGTGTGAC
NNNNNNCTGCTCCGGGATCACATCCTGCCTCCATCTTCCATCTATCCCAGTGTCCTGGCC
TCTGGACCGGATCGTAAAAATAGCAAAACAAGCCAGAAGACCATGGATGATTTAAATTTG
AGCACCTCTGAGGCTCTGCGGATTGACCCTGTTCCTAACACCCACCCACTTCTCGTCTTT
GTCAATCCTAAGAGTGGCGGGAAGCAGGGNNNNAGGGTGCTCTGGAAGTTCCAGTATATA
TTAAACCCTCGACAGGTGTTCAACCTCCTAAAGGATGGTCCTGAGATAGGGCTCCGATTA
TTCAAGGATGTTCCTGATAGCCGGATTTTGGTGTGTGGTGGAGACGGCACAGTAGGCTGG
ATTCTAGAGACCATTGACAAAGCTAACTTGCCAGTTTTGCCTCCTGTTGCTGTGTTGCCC
CTGGGTACTGGAAATGATCTGGCTCGATGCCTAAGATGGGGAGGAGGTTATGAAGGACAG
AATCTGGCAAAGATCCTCAAGGATTTAGAGATGAGTAAAGTGGTACATATGGATCGATGG
TCTGTGGAGGTGATACCTCAACAAACTGAAGAAAAAAGTGACCCAGTCCCCTTTCAAATC
ATCAATAACTACTTCTCTATTGGCGTGGATGCCTCTATTGCTCATCGATTCCACATCATG
CGAGAGAAATATCCGGAGAAGTTCAACAGCAGAATGAAGAACAAGCTATGGTACTTCGAA
TTTGCCACATCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAGGAGTCTTTGACAGTT
GAGATCTGTGGGAAACCGCTGGATCTGAGCAACCTGTCCCTAGAAGGCATCGCAGTGCTA
AACATCCCTAGCATGCATGGTGGCTCCAACCTCTGGGGTGATACCAGGAGACCCCATGGG
GATATCTATGGGATCAACCAGGCCTTAGGTGCTACAGCTAAAGTCATCACCGACCCTGAT
ATCCTGAAAACCTGTGTACCAGACCTAAGTGACAAGAGACTGGAAGTGGTTGGGCTGGAG
GGTGCAATTGAGATGGGCCAAATCTATACCAAGCTCAAGAATGCTGGACGTCGGCTGGCC
AAGTGCTCTGAGATCACCTTCCACACCACAAAAACCCTTCCCATGCAAATTGACGGAGAA
CCCTGGATGCAGACGCCCTGTACAATCAAGATCACCCACAAGAACCAGATGCCCATGCTC
ATGGGCCCACCCCCCCGCTCCACCAATTTCTTTGGCTTCTTGAGCTAA

Clone variation with respect to NM_001345.4
961 t=>n;962 g=>n;963 t=>n;964 g=>n;965 g=>n;966 g=>n;1170 g=>n;1171 c=>n;1172 a=>n;1173 a=>n
>OriGene 5' read for NM_001345 unedited
GTAATTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGAGTGAGTCCC
TAGGCCTCCATCTCTCTCCCTTGCTGTACCACCTTCACCACCATCCATGCGACCCCAAGA
GCCTTAATGACTCTAGAAGAGACTCCAGGCAGGGGAAGCTGAAAGGACCTTTCACTCCCT
ACTTTTGGCCAGGGCCTTCTGTGCCACCTGCCAAGACCAGCAGGCCTACCCTCTGAAGAG
GTCCAAGCAACGGAAGTACTACTACGAAGCTGCCTTTCTGGCCATCCTTGAGAAAAATAG
ACAGATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATA
CATGGAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGAT
GGCTAAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAAT
CTATCTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGA
GACTGGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGGTGGTGTGTCTCAATGATG
TTTCCTGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCT
TCAAGCTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTA
TCCTACAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGA
TTCTTCAGGAGATGATGAAGAGATGCTATGATGGCAGGGGCTCTGTTCTCAACTGGAGGG
GTCCGGGCTGGGGCCACACCGTGCCCTGCTATGCTT
>OriGene 3' read for NM_001345 unedited
GGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGATCCCAAGGGGGAAACA
CAAGTCATTTTATTCAGATGAGGCACTTTCAATGTATGTGTTTTGGGGTGTGTGTATGTG
TGTGCGCATGGGGAACAGTGAGGTGGTGCAGGATAATAAATACTGTGAGGGTGAAGGAAC
ACTCCCCCAAGCTGGCAGGAGTATGTGGCAGCAATGTACAGAGCCTCGGGAGTAGAGTCC
AGGGACAGGGAGGTGGGTTCAAGGCTGGCTTGGAGGCCAAGGGTGTCCCCCTTAGCTCAA
GAAGCCAAAAAAATTGGTGGAGCGGGGGGGTGGGCCCATGAGCATGGGCATCTGGTTCTT
GTGGGTGATCTTGATTGTACAGGGCGTCTGCATCCAGGGTTCTCCGTCAATTTGCATGGG
AAGGGTTTTTGTGGTGTGGAAGGTGATCTCAGAGCACTTGGCCAGCCGACGTCCAGCATT
CTTGAGCTTGGTATAGATTTGGCCCATCTCAATTGCACCCTCCAGCCCAACCACTTCCAG
TCTTTTGTTACTTACGTCTGGTACACAGGTTTTCAGGATATCACGGTCGGTGATGACTTT
AACTGAAGCACCTAACGGCTGGTTGATCCCATATATATCCCCCATGGCGCGCTCCTGGTA
TCACCTCCTAAACGCCGGAGCCCCCATGCGATTGTTAGGGATGGTTACGACCTGCGAACG
CCTCTCCAGGGACAGGGTGCCCAAACCCCCCGGTTTCCCCACACATCTCCCTCCCCCAAC
ACTTCCTTCGTTCTTTTATATGTCGGAAACGAACCCTGTTACCATCCCCAAACANGCGAA
GACATANATAGTGTTCTTTATTTCCTTCCTCTATCTTTGTGGGAAATCGGTGTGGAATTG
GGGGAGAGAGGGGCGCCCCGGCCGCGCCCTCCCATGACATTTCACTCCACCTTCTTCCGC
CCCCCCCATTCGCCCTCCGTCTTCCCCTCCGCCCTCCCCT
Restriction Sites NotI-NotI     
ACCN NM_001345
Insert Size 2800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001345.4, NP_001336.2
RefSeq Size 2756 bp
RefSeq ORF 2208 bp
Locus ID 1606
Cytogenetics 12q13.2
Domains DAGKa, DAGKc, EFh, DAG_PE-bind
Protein Families Druggable Genome
Protein Pathways Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary 'The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]'
Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a.

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