CDK8 (NM_001260) Human Untagged Clone

CAT#: SC119343

CDK8 (untagged)-Human cyclin-dependent kinase 8 (CDK8)


  "NM_001260" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CDK8
Synonyms K35
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_001260 edited
ATGGACTATGACTTTAAAGTGAAGCTGAGCAGCGAGCGGGAGCGGGTCGAGGACCTGTTT
GAATACGAGGGCTGCAAAGTTGGCCGAGGCACTTATGGTCACGTCTACAAAGCCAAGAGG
AAAGATGGGAAGGATGATAAAGACTATGCTTTAAAACAAATAGAAGGAACTGGGATCTCT
ATGTCGGCATGTAGAGAAATAGCATTACTTCGAGAGCTTAAGCATCCAAACGTCATTTCT
CTTCAAAAGGTGTTTCTGTCTCATGCTGATAGGAAGGTGTGGCTTCTGTTTGACTATGCT
GAACATGACCTCTGGCATATAATCAAGTTTCACAGAGCTTCTAAAGCAAACAAGAAGCCA
GTTCAGTTACCTCGGGGAATGGTGAAGTCACTATTATATCAGATCCTAGATGGTATTCAC
TACCTGCATGCTAACTGGGTGTTGCACAGAGATTTGAAACCTGCTAATATTTTAGTTATG
GGTGAAGGTCCTGAGCGAGGAAGAGTAAAAATTGCTGACATGGGCTTTGCCCGATTATTT
AATTCACCTTTGAAGCCTTTAGCAGATTTGGATCCAGTGGTTGTTACATTCTGGTACCGA
GCCCCTGAACTACTTCTTGGAGCAAGGCATTATACCAAAGCTATTGATATTTGGGCTATA
GGGTGTATATTTGCAGAACTACTAACGTCAGAACCAATATTTCACTGTCGACAAGAGGAC
ATCAAAACTAGTAATCCTTATCACCATGACCAGCTGGACAGAATATTCAATGTAATGGGA
TTTCCTGCAGATAAAGATTGGGAAGATATAAAAAAGATGCCTGAACATTCAACATTAATG
AAAGATTTCAGAAGAAATACGTATACCAACTGCAGCCTTATCAAGTATATGGAAAAACAT
AAAGTTAAACCAGATAGTAAAGCATTCCACTTGCTTCAGAAGCTGCTTACCATGGACCCA
ATAAAGCGAATTACCTCAGAACAGGCTATGCAGGACCCCTATTTCTTAGAAGACCCACTT
CCTACATCAGACGTTTTTGCCGGTTGTCAAATCCCTTACCCAAAACGAGAATTTTTAACG
GAAGAAGAACCTGATGACAAAGGAGACAAAAACCAGCAGCAGCAGCAGGGCAATAACCAC
ACTAATGGAACTGGCCACCCAGGGAATCAAGACAGCAGTCACACACAGGGACCCCCGTTG
AAGAAAGTGAGAGTTGTTCCTCCTACCACTACCTCAGGTGGACTTATCATGACCTCAGAC
TATCAGCGTTCCAATCCACATGCTGCCTATCCCAACCCTGGACCAAGCACATCACAGCCG
CAGAGCAGCATGGGATACTCAGCTACCTCCCAGCAGCCTCCACAGTACTCACATCAGACA
CATCGGTACTGA
>OriGene 5' read for NM_001260 unedited
TCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGTGC
GGCCGGCGGGCGTAGAGCGGGCGGGTTCCCGGGGGCTGCGGCTGCCCGTGCTTCCCCGGT
CCCCACCCCTGCCCCCCGGCCCCCCGACCCAGCTCTCCGGCCTCAGAGGCTGTGACAATG
GACTATGACTTTAAAGTGAAGCTGAGCAGCGAGCGGGAGCGGGTCGAGGACCTGTTTGAA
TACGAGGGCTGCAAAGTTGGCCGAGGCACTTATGGTCACGTCTACAAAGCCAAGAGGAAA
GATGGGAAGGATGATAAAGACTATGCTTTAAAACAAATAGAAGGAACTGGGATCTCTATG
TCGGCATGTAGAGAAATAGCATTACTTCGAGAGCTTAAGCATCCAAACGTCATTTCTCTT
CAAAAGGTGTTTCTGTCTCATGCTGATAGGAAGGTGTGGCTTCTGTTTGACTATGCTGAA
CATGACCTCTGGCATATAATCAAGTTTCACAGAGCTTCTAAAGCAAACAAGAAGCCAGTT
CAGTTACCTCGGNGAATGGTGAAGTCACTATTATATCAGATCCTAGATGGTATTCACTAC
CTGCATGCTAACTGGGTGTTGCACAGAGATTTGAAACCTGCTAATATTNTAGTTATGGGT
GAAGGTCCCTGAGCGAGAAAGAGTAAAATGCTGACATGGGCTTTGCCCNGATATTTAATT
CACCTTNGAAGCCTTTAGCAGATTTGGATCCAGTGGGTTGTACATTCTGGTACGGAGCCC
CTGAACTACTNCTGGNNAGCAGGCTTATACCCAAGCTATTGATTTTTGGGCTATAGGGTG
GTATATTTGCAGAACTACTAACGTCAGAACCATATTTNACTGGTCGACAGAGACATCAAA
CTAGTATCCTTATCACCATGACAGCTGGACAGATATCATGGTATGGGATTCCTGCGATAA
GATGGGAAGATTAAAAAGAGCCTGACT
Restriction Sites NotI-NotI     
ACCN NM_001260
Insert Size 2820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001260.1, NP_001251.1
RefSeq Size 1772 bp
RefSeq ORF 1395 bp
Locus ID 1024
Cytogenetics 13q12.13
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase, Transcription Factors
Gene Summary 'This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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