PLOD3 (NM_001084) Human Untagged Clone

CAT#: SC119470

PLOD3 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLOD3
• Synonyms: LH3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119470 sequence for NM_001084 edited (data generated by NextGen Sequencing)
  ATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCCGCTGCTGCTGCCCCCT
  GCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCCAGAGAAGCTGCTG
  GTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCTGCGCTCTGCGGAG
  TTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCGAGGGGGTGATGTG
  GCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGAAATGGAGAAATAC
  GCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGTGATTCTGGCCGGC
  AGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCTGCTCTTCTCTGCA
  GAGAGCTTCTGCTGGCCCGAGTGGGGGCTGGCGGAGCAGTACCCTGAGGTGGGCACGGGG
  AAGCGCTTCCTCAATTCTGGTGGATTCATCGGTTTTGCCACCACCATCCACCAAATCGTG
  CGCCAGTGGAAGTACAAGGATGATGACGACGACCAGCTGTTCTACACACGGCTCTACCTG
  GACCCAGGACTGAGGGAGAAACTCAGCCTTAATCTGGATCATAAGTCTCGGATCTTTCAG
  AACCTCAACGGGGCTTTAGATGAAGTGGTTTTAAAGTTTGATCGGAACCGTGTGCGTATC
  CGGAACGTGGCCTACGACACGCTCCCCATTGTGGTCCATGGAAACGGTCCCACTAAGCTG
  CAGCTCAACTACCTGGGAAACTACGTCCCCAATGGCTGGACTCCTGAGGGAGGCTGTGGC
  TTCTGCAACCAGGACCGGAGGACACTCCCGGGGGGGCAGCCTCCCCCCCGGGTGTTTCTG
  GCCGTGTTTGTGGAACAGCCTACTCCGTTTCTGCCCCGCTTCCTGCAGCGGCTGCTACTC
  CTGGACTATCCCCCCGACAGGGTCACCCTTTTCCTGCACAACAACGAGGTCTTCCATGAA
  CCCCACATCGCTGACTCCTGGCCGCAGCTCCAGGACCACTTCTCAGCTGTGAAGCTCGTG
  GGGCCGGAGGAGGCTCTGAGCCCAGGCGAGGCCAGGGACATGGCCATGGACCTGTGTCGG
  CAGGACCCCGAGTGTGAGTTCTACTTCAGCCTGGACGCCGACGCTGTCCTCACCAACCTG
  CAGACCCTGCGTATCCTCATTGAGGAGAACAGGAAGGTGATCGCCCCCATGCTGTCCCGC
  CACGGCAAGCTGTGGTCCAACTTCTGGGGCGCCCTGAGCCCCGATGAGTACTACGCCCGC
  TCCGAGGACTACGTGGAGCTGGTGCAGCGGAAGCGAGTGGGTGTGTGGAATGTACCATAC
  ATCTCCCAGGCCTATGTGATCCGGGGTGATACCCTGCGGATGGAGCTGCCCCAGAGGGAT
  GTGTTCTCGGGCAGTGACACAGACCCGGACATGGCCTTCTGTAAGAGCTTTCGAGACAAG
  GGCATCTTCCTCCATCTGAGCAATCAGCATGAATTTGGCCGGCTCCTGGCCACTTCCAGA
  TACGACACGGAGCACCTGCACCCCGACCTCTGGCAGATCTTCGACAACCCCGTCGACTGG
  AAGGAGCAGTACATCCACGAGAACTACAGCCGGGCCCTGGAAGGGGAAGGAATCGTGGAG
  CAGCCATGCCCGGACGTGTACTGGTTCCCACTGCTGTCAGAACAAATGTGTGATGAGCTG
  GTGGCAGAGATGGAGCACTACGGCCAGTGGTCAGGCGGCCGGCATGAGGATTCAAGGCTG
  GCTGGAGGCTACGAGAATGTGCCCACCGTGGACATCCACATGAAGCAGGTGGGGTACGAG
  GACCAGTGGCTGCAGCTGCTGCGGACGTATGTGGGCCCCATGACCGAGAGCCTGTTTCCC
  GGTTACCACACCAAGGCGCGGGCGGTGATGAACTTTGTGGTTCGCTACCGGCCAGACGAG
  CAGCCGTCTCTGCGGCCACACCACGACTCATCCACCTTCACCCTCAACGTTGCCCTCAAC
  CACAAGGGCCTGGACTATGAGGGAGGTGGCTGCCGCTTCCTGCGCTACGACTGTGTGATC
  TCCTCCCCGAGGAAGGGCTGGGCACTCCTGCACCCCGGCCGCCTCACCCACTACCACGAG
  GGGCTGCCAACGACCTGGGGCACACGCTACATCATGGTGTCCTTTGTCGACCCCTGA
  
  Clone variation with respect to NM_001084.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001084 unedited
  GGTGGATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCTGTGGCGC
  CGGCATCTGGAGCTTTCTGTAGCCTCCGGATACGCCTTTTTTTCAGGGCGTAGCCCCAGC
  CAAGCTGCTCCCCGCGGCGGCCGCACAGCAGCCCGAGCGCCCCCTTTCCAGAGCTCCCCT
  CCGGAGCTGGGATCCAGGCGCGTAGCGGAGATCCCAGGATCCTGGGTGCTGTCTGGGCCC
  GCTCCCCACCATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCCGCTGCT
  GCTGCCCCCTGCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCCAGA
  GAAGCTGCTGGTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCTGCG
  CTCTGCGGAGTTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCGAGG
  GGGTGATGTGGCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGAAAT
  GGAGAAATACGCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGTGAT
  TCTGGCCGGCAGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCTGCT
  CTTCTCTGCAGAGAGCTTCTGCTGGCCCGAGTGGGGGCTGGCGGAGCAGTACCCTGAGGT
  GGGCACGGGGAAAGCGCTTCCTCCATTCTTGTGGATTCATCCGGTTTTGCCACACCATCC
  ACCATATCGTGCGCCAGTGGCAGTACAANGATGATGACGACGACCCGCTGTTCTACACAC
  GCTCTACCTGGAACCAGGACTGAAGGAGGAACTCACCCTTATCCTGGATCTAAGTCTTCG
  GATCTTTCAGAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001084 unedited
  GCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACTTACAAAC
  TGTCCTTTATTCAAAGGGAGACTGCGGAACATTAATAATTTATCACGCGGGGAGTCCCCA
  AAAGCCCTGTGCCCACGAACCCCTGTGGGCGGAGGAAAAAGGCGGGGACTCCGGGAGCTT
  CCTGAGAGGGCCGTGTCTTGGGAGCAAGGTGACATATTCAGTTCAGGCACGCGGAACATG
  AACTCAGGAAGGGGGGAGACAGAGAGACCCATCCCCCAACTCCCAGGACGGGGGCCAGGC
  CCCCTAAAAAGGCACATTGGCAGGGCAGGTTTGGCAAAGGGGTTGAGTGTCAGGGGTCGA
  CAAAGGACACCATGATGTAGCGTGTGCCCCAGGTCGTTGGCAGCCCCTCGTGGTATTGGG
  TGAGGCGGCCGGGGTGCAGGATTGCCCACCCCTTCCTCGGGGAGGAGATCACACAGTCGT
  AGCGCAGGAAGCGGCAGCCACCTCCCTCATAGTCCAGGCCCTTGTGGTTGAGGGCAACGT
  TGAGGGTGAAGGTGGATGATTCGTGGTGTGGCCGCATAGACGGCTGCTCGTCTGGCCGGT
  AGCGAACCACAAAGTTCATCACCGCCCGCGCCTTGTTGTGGAACCCGGGAAACGGCCTCT
  CGGTCATGGGGCCCACATACGTCCGCAGCAGCTGCAGCCCCTGGTCCTCGTACCCACCTG
  CTTCATGTGAATGTCACCGTGGGCACTTTCTCGTAGCCTCAGCCAGCCTTGATTCTAATG
  CCGGCGCCTGACCCTGGCCGTAGGCTCATTTTTGCACCAGCTTATAAAATTTGTTTTGAC
  GGAGTGGGACCATTCCCGTCCGGCATGTTTGTCCAGTATTCTTCCCTCCAGGGCCAGCTG
  TATTCTCGGGGAGACCGCTCCTCACCCAGGGNTTGCAAATACTGCAAGGCGGGGGAGTGC
  TCCGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001084
• ORF Size: 2217 bp
• Insert Size: 2710
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_001084.4, NP_001075.1
• RefSeq Size: 2995
• RefSeq ORF: 2217
• Locus ID: 8985
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Pathways: Lysine degradation
• Gene Summary: The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]