PLOD3 (NM_001084) Human Untagged Clone

CAT#: SC324563

PLOD3 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3)


  "NM_001084" in other vectors (5)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PLOD3
Synonyms LH3
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_001084.4 GCTTCCACATGTGTCAAGCGGCTGGCTCAGCCCAGAGTCCCTGTCTCCCGCCCGCCGGCC
CGAGCCGCCGCCCCTCCCCCGCCTCCCGTGCGCCCGGGACAATCCTCGCCTTGTCTGTGG
CGCCGGCATCTGGAGCTTTCTGTAGCCTCCGGATACGCCTTTTTTTCAGGGCGTAGCCCC
AGCCAAGCTGCTCCCCGCGGCGGCCGCACAGCAGCCCGAGCGCCCCCTTTCCGGAGCTCC
CCTCCGGAGCTGGGATCCAGGCGCGTAGCGGAGATCCCAGGATCCTGGGTGCTGTCTGGG
CCCGCTCCCCACCATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCCGCT
GCTGCTGCCCCCTGCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCC
AGAGAAGCTGCTGGTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCT
GCGCTCTGCGGAGTTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCG
AGGGGGTGATGTGGCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGA
AATGGAGAAATACGCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGT
GATTCTGGCCGGCAGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCT
GCTCTTCTCTGCAGAGAGCTTCTGCTGGCCCGAGTGGGGGCTGGCGGAGCAGTACCCTGA
GGTGGGCACGGGGAAGCGCTTCCTCAATTCTGGTGGATTCATCGGTTTTGCCACCACCAT
CCACCAAATCGTGCGCCAGTGGAAGTACAAGGATGATGACGACGACCAGCTGTTCTACAC
ACGGCTCTACCTGGACCCAGGACTGAGGGAGAAACTCAGCCTTAATCTGGATCATAAGTC
TCGGATCTTTCAGAACCTCAACGGGGCTTTAGATGAAGTGGTTTTAAAGTTTGATCGGAA
CCGTGTGCGTATCCGGAACGTGGCCTACGACACGCTCCCCATTGTGGTCCATGGAAACGG
TCCCACTAAGCTGCAGCTCAACTACCTGGGAAACTACGTCCCCAATGGCTGGACTCCTGA
GGGAGGCTGTGGCTTCTGCAACCAGGACCGGAGGACACTCCCGGGGGGGCAGCCTCCCCC
CCGGGTGTTTCTGGCCGTGTTTGTGGAACAGCCTACTCCGTTTCTGCCCCGCTTCCTGCA
GCGGCTGCTACTCCTGGACTATCCCCCCGACAGGGTCACCCTTTTCCTGCACAACAACGA
GGTCTTCCATGAACCCCACATCGCTGACTCCTGGCCGCAGCTCCAGGACCACTTCTCAGC
TGTGAAGCTCGTGGGGCCGGAGGAGGCTCTGAGCCCAGGCGAGGCCAGGGACATGGCCAT
GGACCTGTGTCGGCAGGACCCCGAGTGTGAGTTCTACTTCAGCCTGGACGCCGACGCTGT
CCTCACCAACCTGCAGACCCTGCGTATCCTCATTGAGGAGAACAGGAAGGTGATCGCCCC
CATGCTGTCCCGCCACGGCAAGCTGTGGTCCAACTTCTGGGGCGCCCTGAGCCCCGATGA
GTACTACGCCCGCTCCGAGGACTACGTGGAGCTGGTGCAGCGGAAGCGAGTGGGTGTGTG
GAATGTACCATACATCTCCCAGGCCTATGTGATCCGGGGTGATACCCTGCGGATGGAGCT
GCCCCAGAGGGATGTGTTCTCGGGCAGTGACACAGACCCGGACATGGCCTTCTGTAAGAG
CTTTCGAGACAAGGGCATCTTCCTCCATCTGAGCAATCAGCATGAATTTGGCCGGCTCCT
GGCCACTTCCAGATACGACACGGAGCACCTGCACCCCGACCTCTGGCAGATCTTCGACAA
CCCCGTCGACTGGAAGGAGCAGTACATCCACGAGAACTACAGCCGGGCCCTGGAAGGGGA
AGGAATCGTGGAGCAGCCATGCCCGGACGTGTACTGGTTCCCACTGCTGTCAGAACAAAT
GTGTGATGAGCTGGTGGCAGAGATGGAGCACTACGGCCAGTGGTCAGGCGGCCGGCATGA
GGATTCAAGGCTGGCTGGAGGCTACGAGAATGTGCCCACCGTGGACATCCACATGAAGCA
GGTGGGGTACGAGGACCAGTGGCTGCAGCTGCTGCGGACGTATGTGGGCCCCATGACCGA
GAGCCTGTTTCCCGGTTACCACACCAAGGCGCGGGCGGTGATGAACTTTGTGGTTCGCTA
CCGGCCAGACGAGCAGCCGTCTCTGCGGCCACACCACGACTCATCCACCTTCACCCTCAA
CGTTGCCCTCAACCACAAGGGCCTGGACTATGAGGGAGGTGGCTGCCGCTTCCTGCGCTA
CGACTGTGTGATCTCCTCCCCGAGGAAGGGCTGGGCACTCCTGCACCCCGGCCGCCTCAC
CCACTACCACGAGGGGCTGCCAACGACCTGGGGCACACGCTACATCATGGTGTCCTTTGT
CGACCCCTGACACTCAACCACTCTGCCAAACCTGCCCTGCCATTGTGCCTTTTTAGGGGG
CCTGGCCCCCGTCCTGGGAGTTGGGGGATGGGTCTCTCTGTCTCCCCACTTCCTGAGTTC
ATGTTCCGCGTGCCTGAACTGAATATGTCACCTTGCTCCCAAGACACGGCCCTCTCAGGA
AGCTCCCGGAGTCCCCGCCTCTCTCCTCCGCCCACAGGGGTTCGTGGGCACAGGGCTTCT
GGGGACTCCCCGCGTGATAAATTATTAATGTTCCGCAGTCTCACTCTGAATAAAGGACAG
TTTGTAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_001084.4 unedited
GGCCATTGTATACGACTCCTATAGGGCGGCCGGGAATCGGCACGAGGGCTTCCACATGTG
TCAAGCGGCTGGCTCAGCCCAGAGTCCCTGTCTCCCGCCCGCCGGCCCGAGCCGCCGCCC
CTCCCCCGCCTCCCGTGCGCCCGGGACAATCCTCGCCTTGTCTGTGGCGCCGGCATCTGG
AGCTTTCTGTAGCCTCCGGATACGCCTTTTTTTCAGGGCGTAGCCCCAGCCAAGCTGCTC
CCCGCGGCGGCCGCACAGCAGCCCGAGCGCCCCCTTTCCGGAGCTCCCCTCCGGAGCTGG
GATCCAGGCGCGTAGCGGAGATCCCAGGATCCTGGGTGCTGTCTGGTTCCCGCTCCCCAC
CATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCTTCTGCTGCTGCCCCC
TGCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCCAGAGAAGCTGCT
GGTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCTGCGCTCTGCGGA
GTTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCGAGGGGGTGATGT
GGCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGAAATGGAGAAATA
CGCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGTGATTCTGGCCGG
CAGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCTGCTCTTCTCTGC
AGAGAGCTTCTGCTGGCCCGAGTGGGGGCTGCGAGCAGTACCCTGAGTGGGCACGGGGAA
GCGCTTCCTCAATTCTGTGATTCATCGGTTTTGCACACCATCCACCCAATCGTGGCGCCA
GTGAAGTACAGGATGATGACGACGAACCAGCTGTTCT
Restriction Sites Please inquire     
ACCN NM_001084
ORF Size 2217 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Reference Data
RefSeq NM_001084.4, NP_001075.1
RefSeq Size 2995
RefSeq ORF 2217
Locus ID 8985
Domains 2OG-FeII_Oxy, P4Hc
Protein Pathways Lysine degradation
Gene Summary The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]

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