PLOD3 (NM_001084) Human Untagged Clone

CAT#: SC324563

PLOD3 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLOD3
• Synonyms: LH3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001084.4 GCTTCCACATGTGTCAAGCGGCTGGCTCAGCCCAGAGTCCCTGTCTCCCGCCCGCCGGCC
  CGAGCCGCCGCCCCTCCCCCGCCTCCCGTGCGCCCGGGACAATCCTCGCCTTGTCTGTGG
  CGCCGGCATCTGGAGCTTTCTGTAGCCTCCGGATACGCCTTTTTTTCAGGGCGTAGCCCC
  AGCCAAGCTGCTCCCCGCGGCGGCCGCACAGCAGCCCGAGCGCCCCCTTTCCGGAGCTCC
  CCTCCGGAGCTGGGATCCAGGCGCGTAGCGGAGATCCCAGGATCCTGGGTGCTGTCTGGG
  CCCGCTCCCCACCATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCCGCT
  GCTGCTGCCCCCTGCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCC
  AGAGAAGCTGCTGGTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCT
  GCGCTCTGCGGAGTTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCG
  AGGGGGTGATGTGGCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGA
  AATGGAGAAATACGCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGT
  GATTCTGGCCGGCAGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCT
  GCTCTTCTCTGCAGAGAGCTTCTGCTGGCCCGAGTGGGGGCTGGCGGAGCAGTACCCTGA
  GGTGGGCACGGGGAAGCGCTTCCTCAATTCTGGTGGATTCATCGGTTTTGCCACCACCAT
  CCACCAAATCGTGCGCCAGTGGAAGTACAAGGATGATGACGACGACCAGCTGTTCTACAC
  ACGGCTCTACCTGGACCCAGGACTGAGGGAGAAACTCAGCCTTAATCTGGATCATAAGTC
  TCGGATCTTTCAGAACCTCAACGGGGCTTTAGATGAAGTGGTTTTAAAGTTTGATCGGAA
  CCGTGTGCGTATCCGGAACGTGGCCTACGACACGCTCCCCATTGTGGTCCATGGAAACGG
  TCCCACTAAGCTGCAGCTCAACTACCTGGGAAACTACGTCCCCAATGGCTGGACTCCTGA
  GGGAGGCTGTGGCTTCTGCAACCAGGACCGGAGGACACTCCCGGGGGGGCAGCCTCCCCC
  CCGGGTGTTTCTGGCCGTGTTTGTGGAACAGCCTACTCCGTTTCTGCCCCGCTTCCTGCA
  GCGGCTGCTACTCCTGGACTATCCCCCCGACAGGGTCACCCTTTTCCTGCACAACAACGA
  GGTCTTCCATGAACCCCACATCGCTGACTCCTGGCCGCAGCTCCAGGACCACTTCTCAGC
  TGTGAAGCTCGTGGGGCCGGAGGAGGCTCTGAGCCCAGGCGAGGCCAGGGACATGGCCAT
  GGACCTGTGTCGGCAGGACCCCGAGTGTGAGTTCTACTTCAGCCTGGACGCCGACGCTGT
  CCTCACCAACCTGCAGACCCTGCGTATCCTCATTGAGGAGAACAGGAAGGTGATCGCCCC
  CATGCTGTCCCGCCACGGCAAGCTGTGGTCCAACTTCTGGGGCGCCCTGAGCCCCGATGA
  GTACTACGCCCGCTCCGAGGACTACGTGGAGCTGGTGCAGCGGAAGCGAGTGGGTGTGTG
  GAATGTACCATACATCTCCCAGGCCTATGTGATCCGGGGTGATACCCTGCGGATGGAGCT
  GCCCCAGAGGGATGTGTTCTCGGGCAGTGACACAGACCCGGACATGGCCTTCTGTAAGAG
  CTTTCGAGACAAGGGCATCTTCCTCCATCTGAGCAATCAGCATGAATTTGGCCGGCTCCT
  GGCCACTTCCAGATACGACACGGAGCACCTGCACCCCGACCTCTGGCAGATCTTCGACAA
  CCCCGTCGACTGGAAGGAGCAGTACATCCACGAGAACTACAGCCGGGCCCTGGAAGGGGA
  AGGAATCGTGGAGCAGCCATGCCCGGACGTGTACTGGTTCCCACTGCTGTCAGAACAAAT
  GTGTGATGAGCTGGTGGCAGAGATGGAGCACTACGGCCAGTGGTCAGGCGGCCGGCATGA
  GGATTCAAGGCTGGCTGGAGGCTACGAGAATGTGCCCACCGTGGACATCCACATGAAGCA
  GGTGGGGTACGAGGACCAGTGGCTGCAGCTGCTGCGGACGTATGTGGGCCCCATGACCGA
  GAGCCTGTTTCCCGGTTACCACACCAAGGCGCGGGCGGTGATGAACTTTGTGGTTCGCTA
  CCGGCCAGACGAGCAGCCGTCTCTGCGGCCACACCACGACTCATCCACCTTCACCCTCAA
  CGTTGCCCTCAACCACAAGGGCCTGGACTATGAGGGAGGTGGCTGCCGCTTCCTGCGCTA
  CGACTGTGTGATCTCCTCCCCGAGGAAGGGCTGGGCACTCCTGCACCCCGGCCGCCTCAC
  CCACTACCACGAGGGGCTGCCAACGACCTGGGGCACACGCTACATCATGGTGTCCTTTGT
  CGACCCCTGACACTCAACCACTCTGCCAAACCTGCCCTGCCATTGTGCCTTTTTAGGGGG
  CCTGGCCCCCGTCCTGGGAGTTGGGGGATGGGTCTCTCTGTCTCCCCACTTCCTGAGTTC
  ATGTTCCGCGTGCCTGAACTGAATATGTCACCTTGCTCCCAAGACACGGCCCTCTCAGGA
  AGCTCCCGGAGTCCCCGCCTCTCTCCTCCGCCCACAGGGGTTCGTGGGCACAGGGCTTCT
  GGGGACTCCCCGCGTGATAAATTATTAATGTTCCGCAGTCTCACTCTGAATAAAGGACAG
  TTTGTAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001084.4 unedited
  GGCCATTGTATACGACTCCTATAGGGCGGCCGGGAATCGGCACGAGGGCTTCCACATGTG
  TCAAGCGGCTGGCTCAGCCCAGAGTCCCTGTCTCCCGCCCGCCGGCCCGAGCCGCCGCCC
  CTCCCCCGCCTCCCGTGCGCCCGGGACAATCCTCGCCTTGTCTGTGGCGCCGGCATCTGG
  AGCTTTCTGTAGCCTCCGGATACGCCTTTTTTTCAGGGCGTAGCCCCAGCCAAGCTGCTC
  CCCGCGGCGGCCGCACAGCAGCCCGAGCGCCCCCTTTCCGGAGCTCCCCTCCGGAGCTGG
  GATCCAGGCGCGTAGCGGAGATCCCAGGATCCTGGGTGCTGTCTGGTTCCCGCTCCCCAC
  CATGACCTCCTCGGGGCCTGGACCCCGGTTCCTGCTGCTGCTGCTTCTGCTGCTGCCCCC
  TGCGGCCTCAGCCTCCGACCGGCCCCGGGGCCGAGACCCGGTCAACCCAGAGAAGCTGCT
  GGTGATCACTGTGGCCACAGCTGAAACCGAGGGGTACCTGCGTTTCCTGCGCTCTGCGGA
  GTTCTTCAACTACACTGTGCGGACCCTGGGCCTGGGAGAGGAGTGGCGAGGGGGTGATGT
  GGCTCGAACAGTTGGTGGAGGACAGAAGGTCCGGTGGTTAAAGAAGGAAATGGAGAAATA
  CGCTGACCGGGAGGATATGATCATCATGTTTGTGGATAGCTACGACGTGATTCTGGCCGG
  CAGCCCCACAGAGCTGCTGAAGAAGTTCGTCCAGAGTGGCAGCCGCCTGCTCTTCTCTGC
  AGAGAGCTTCTGCTGGCCCGAGTGGGGGCTGCGAGCAGTACCCTGAGTGGGCACGGGGAA
  GCGCTTCCTCAATTCTGTGATTCATCGGTTTTGCACACCATCCACCCAATCGTGGCGCCA
  GTGAAGTACAGGATGATGACGACGAACCAGCTGTTCT
• Restriction Sites: Please inquire
• ACCN: NM_001084
• ORF Size: 2217 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001084.4, NP_001075.1
• RefSeq Size: 2995
• RefSeq ORF: 2217
• Locus ID: 8985
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Pathways: Lysine degradation
• Gene Summary: The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]