SCF (KITLG) (NM_000899) Human Untagged Clone

CAT#: SC119596

KITLG (untagged)-Human KIT ligand (KITLG), transcript variant b


  "NM_000899" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol KITLG
Synonyms DCUA; DFNA69; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; SLF
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119596 sequence for NM_000899 edited (data generated by NextGen Sequencing)
ATGAAGAAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAAT
CCTCTCGTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGTC
ACTAAATTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGGG
ATGGATGTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAGC
TTGACTGATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCATC
ATAGACAAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATCT
AAGGATCTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATTC
TTTAGAATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAACT
AGTGATTGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGATTCCAGAGTCAGTGTCACA
AAACCATTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGT
AATAGGAAGGCCAAAAATCCCCCTGGAGACTCCAGCCTACACTGGGCAGCCATGGCATTG
CCAGCATTGTTTTCTCTTATAATTGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAGA
CAGCCAAGTCTTACAAGGGCAGTTGAAAATATACAAATTAATGAAGAGGATAATGAGATA
AGTATGTTGCAAGAGAAAGAGAGAGAGTTTCAAGAAGTGTAA

Clone variation with respect to NM_000899.4
>OriGene 5' read for NM_000899 unedited
GGGTGCAAATTATGTATACGACTCACTTAGGCGGCCGCGAAATTCGCACGAGGGAACAGC
TAAACGGAGTCGCCACACCACTGTTTGTGCTGGATCGCAGCGCTGCCTTTCCTTATGAAG
AAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAATCCTCTC
GTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGTCACTAAA
TTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGGGATGGAT
GTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAGCTTGACT
GATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCATCATAGAC
AAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATCTAAGGAT
CTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATTCTTTAGA
ATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAACTAGTGAT
TGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGATTCCAGAGTCAGTGTCACAAAACCA
TTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTANGAATGACAGCAGTAGCAGTAATAGG
AAGGCCAAAAATCCCCCTGGAGACTCCAGCCTACACTGNGCAGCCATGGCATTGCCAGCA
TTGTTTTCTCTTATAATTGGCTNTGCTTTTGGAGCCCTATACTTGGAGAAGAGACAGCCA
AGTCTTACAAGGGCAGTTGAAATATACCAATAATGAAGAGGATATGAGATAAGTTGTTGC
AAGAAAAGAGAGAGAGTTCAAGAGTGTTATGTG
>OriGene 3' read for NM_000899 unedited
GGTTTAACTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTGAGAGTGCATAAAATTCTTTATTTAGAACAATAAAGTATATGTATATAT
CATGTAATTTTAAATTCAAACCTGTATAAAAAAGGGTTGGGACATACACCTATTTTAAAA
AGTACACATACCGTATATACACATACCCATCACATTTTACAACCTTTTTTATTTAATTAA
ATTTCAGTCATAATAGAACTATTTAAAATAGTGTCGACATACATTTACATTATTATATGC
AAACTCCAATCTATGACTGAACTGAGGGTATATCTGCGCATCCATCTAAATTAATACTTT
TCAAGTCCATTGATTTAAAAAAATGGTGGCAAGTGGACACTATAATCACATATTAACCCT
TAGAATATAGAATAGAATATTTTGATTGGAAAATTATATTTAGTGAGTATTGTAGCTCAA
AGATCAAGTACTGGAAAACGGTGTGGTTTTTAGTTTAACAGTTGTTTTAATTTTATTGTT
AAAAACATCATAAGGATGTAGTTGGAGATGGCAGTTATAAATAAATTTACAATGCTTAGT
AAACATTTCAGGATCACAAATATCCATTTTAGCATGGATTATTTCTTGAAAAAAACACCT
TGTTATAAAATGAAAGTACCATTATTTTATTTTTTACAACAAAAGCATTTCAAGACTAGT
TAATGGGAAGCAGCACCTTATGAATTGGTGATCAAACTCACCAATGAACTATTAGAATTT
CAATCCGAATGTTTTATTTTAAAGCCCATTTCAAATAATATTGTTTCAAAGAAAAACTGG
TTACTTTAAATTCTTAAACCCTTTCCCATTTTAAAGGGAATTTAAGAAATCACCCTTTTA
AATGA
Restriction Sites NotI-NotI     
ACCN NM_000899
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000899.3, NP_000890.1
RefSeq Size 5435 bp
RefSeq ORF 822 bp
Locus ID 4254
Cytogenetics 12q21.32
Domains SCF
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer
Gene Summary 'This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (b) encodes the longer isoform (b) which contains the primary proteolytic-cleavage site; as a result, isoform b is largely a soluble product. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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