NPY1R (NM_000909) Human Untagged Clone

CAT#: SC119604

NPY1R (untagged)-Human neuropeptide Y receptor Y1 (NPY1R)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPY1R
• Synonyms: NPY1-R; NPYR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119604 sequence for NM_000909 edited (data generated by NextGen Sequencing)
  ATGAATTCAACATTATTTTCCCAGGTTGAAAATCATTCAGTCCACTCTAATTTCTCAGAG
  AAGAATGCCCAGCTTCTGGCTTTTGAAAATGATGATTGTCATCTGCCCTTGGCCATGATA
  TTTACCTTAGCTCTTGCTTATGGAGCTGTGATCATTCTTGGTGTCTCTGGAAACCTGGCC
  TTGATCATAATCATCTTGAAACAAAAGGAGATGAGAAATGTTACCAACATCCTGATTGTG
  AACCTTTCCTTCTCAGACTTGCTTGTTGCCATCATGTGTCTCCCCTTTACATTTGTCTAC
  ACATTAATGGACCACTGGGTCTTTGGTGAGGCGATGTGTAAGTTGAATCCTTTTGTGCAA
  TGTGTTTCAATCACTGTGTCCATTTTCTCTCTGGTTCTCATTGCTGTGGAACGACATCAG
  CTGATAATCAACCCTCGAGGGTGGAGACCAAATAATAGACATGCTTATGTAGGTATTGCT
  GTGATTTGGGTCCTTGCTGTGGCTTCTTCTTTGCCTTTCCTGATCTACCAAGTAATGACT
  GATGAGCCGTTCCAAAATGTAACACTTGATGCGTACAAAGACAAATACGTGTGCTTTGAT
  CAATTTCCATCGGACTCTCATAGGTTGTCTTATACCACTCTCCTCTTGGTGCTGCAGTAT
  TTTGGTCCACTTTGTTTTATATTTATTTGCTACTTCAAGATATATATACGCCTAAAAAGG
  AGAAACAACATGATGGACAAGATGAGAGACAATAAGTACAGGTCCAGTGAAACCAAAAGA
  ATCAATATCATGCTGCTCTCCATTGTGGTAGCATTTGCAGTCTGCTGGCTCCCTCTTACC
  ATCTTTAACACTGTGTTTGATTGGAATCATCAGATCATTGCTACCTGCAACCACAATCTG
  TTATTCCTGCTCTGCCACCTCACAGCAATGATATCCACTTGTGTCAACCCCATATTTTAT
  GGGTTCCTGAACAAAAACTTCCAGAGAGACTTGCAGTTCTTCTTCAACTTTTGTGATTTC
  CGGTCTCGGGATGATGATTATGAAACAATAGCCATGTCCACGATGCACACAGATGTTTCC
  AAAACTTCTTTGAAGCAAGCAAGCCCAGTCGCATTTAAAAAAATCAACAACAATGATGAT
  AATGAAAAAATCTGA
  
  Clone variation with respect to NM_000909.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000909 unedited
  CCCAATTACNATTTGTATACGACTTTTATAGGCGGCCGCGAATCGCACGAGGGAGGTCTG
  TGTCGAATAATGTGTGGCTTCTGTTGGATTGCTTTTCTTTCCAAAATTCCTAGGCAATGC
  TTCCCCGAGGTGTGCACCTTTGTGAGGTGTTTGTGGGGTTGGGGGAGCTTCAGGCGCTAC
  TCGCGGGACGCCGTCACGTGATCCGGGATGAGGTGGAGTTCGGCTTTAAGGAGGCGTCTC
  TTCCTAGCTTCATCAATCTTTAGGATCTGAGCAGGAGAAATACCAGCGGATCTTCCCCAC
  TCTGCTCCCTTCCATTCCCACCCTTCCTTCTTTAATAAGCAGGAGCGAAAAAGACAAATT
  CCAAAGAGGATTGTTCAGTTCAAGGGAATGAAGAATTCAGAATAATTTTGGTAAATGGAT
  TCCAATATGGGGAATAAGAATAAGCTGAACAGTTGACCTGCTTTGAAGAAACATACTGTC
  CATTTGTCTAAAATAATCTATAACAACCAAACCAATCAAAATGAATTCAACATTATTTTC
  CCAGGTTGAAAATCATTCAGTCCACTCTAATTTCTCAGAGAAGAATGCCCAGCTTCTGGC
  TTTTGAAAATGATGATTGTCATCTGCCCTTGGCCATGATATTTACCTTAGCTCTTGCTTA
  TGGAGCTGTGATCATTCTTGGTGTCTCTGGCAACCTGGCCTTGATCATAATCATCTTGAA
  ACTAAAGAGATGAGAAATGTTACCAACATCCTGATTGTGAACCTTTCCTTCTCAGACTTG
  CTTGGTGCCATCATGTGTCTCCCCTATACATTTGTCTACCATTAATGGACCACTGGGTCT
  TTGGTGAGGCGATGTGTAAGTTGAAACCCTTTGTGCATGTGTTTCAACACTGGGGCCATT
  TCTCTCTGGTCTCATGCTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000909 unedited
  ACAATATATGCATCTTTAATTAAAATGTNCACGCCCTCTTTAAAATCAAACACATGTAAT
  ACTTTATTCTGTATAATACATTGAAGTATGTCTTGTTCTTTAAAATATAGTTTCTCCAAA
  TGATTCTGAGGCTCAATCATGATTACAAGATACATGAAATTAGGCACATTAACATGACAC
  AACTATTCAGTAATCTTTACAGTGCATTATTTATAAGACGCCACAAGTCACACAGTTTAT
  ACAAGTTAAAATGGGCTATATGAAGTATCTGCAGTTTTTGTGTATTTTTCCCTGCTAGGT
  AAAGTGAATATTCCTTTAACATTGTACCTTGGTGTTACTTCCTTTGAAAGATGGACATTG
  AAAGTTAAAAATCAGTCACTATATAGGCACTGCTGTATGGAAAACGCATTTTGTGTTTCT
  ACAAATTGTAAGCGGAAATGCCTTTTGAGTACATATTTATGCCAGAAAGCTTGACAAACA
  CTGAACAGTCTGTAAAAAAAATGGAAAATCTCTTGACCATCAATTCAAATAAATATAATT
  TAACTATTTGCAGCAATACTAATTTACTTTATTAAATTAATTACTCTAAAAAGCTCTTGT
  TGTCAAGTACCAAAATGGTTTTGAAGTTAAGGAGCTGCTTTCTCTCTTCATTCCTGTGGG
  TGCCCCAATTCTCCATAATATTTGCAGCTGAGAAGTATGTCTTTTTATTCATTCTTAAAT
  TTTCATCTAAGTGACCATCAGTTGGGAGCAAATTAAATCCTCCCTCAGGTGAAAAAAATA
  AAAAACAAACAATAACCACCAAAAAACAGAAACCTCAAACCACTTCTGAATGACTTCAGA
  CTATCTTTCGGCTCCTGAATGCTGCCTTTGCATACTGTTACTATTGGAACACACTAAAAT
  GATGCCCTATCATAAGTAAGGATGGCCCAATCGTTGACATTCAATCAATCTAATCAATCA
  GATGACCCATCTATGACCGTTGGATGGAAGTCCTCTGAGTAGCTAATGAATACTTTAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000909
• Insert Size: 3370 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000909.4, NP_000900.1
• RefSeq Size: 2775 bp
• RefSeq ORF: 1155 bp
• Locus ID: 4886
• Cytogenetics: 4q32.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene belongs to the G-protein-coupled receptor superfamily. The encoded transmembrane protein mediates the function of neuropeptide Y (NPY), a neurotransmitter, and peptide YY (PYY), a gastrointestinal hormone. The encoded receptor undergoes fast agonist-induced internalization through clathrin-coated pits and is subsequently recycled back to the cell membrane. Activation of Y1 receptors may result in mobilization of intracellular calcium and inhibition of adenylate cyclase activity. [provided by RefSeq, Aug 2013]'