P4HB (NM_000918) Human Untagged Clone

CAT#: SC119620

P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB)


  "NM_000918" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol P4HB
Synonyms CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119620 sequence for NM_000918 edited (data generated by NextGen Sequencing)
ATGCTGCGCCGCGCTCTGCTGTGCCTGGCCGTGGCCGCCCTGGTGCGCGCCGACGCCCCC
GAGGAGGAGGACCACGTCCTGGTGCTGCGGAAAAGCAACTTCGCGGAGGCGCTGGCGGCC
CACAAGTACCTGCTGGTGGAGTTCTATGCCCCTTGGTGTGGCCACTGCAAGGCTCTGGCC
CCTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATCAGGTTGGCC
AAGGTGGACGCCACGGAGGAGTCTGACCTGGCCCAGCAGTACGGCGTGCGCGGCTATCCC
ACCATCAAGTTCTTCAGGAATGGAGACACGGCTTCCCCCAAGGAATATACAGCTGGCAGA
GAGGCTGATGACATCGTGAACTGGCTGAAGAAGCGCACGGGCCCGGCTGCCACCACCCTG
CCTGACGGCGCAGCTGCAGAGTCCTTGGTGGAGTCCAGCGAGGTGGCTGTCATCGGCTTC
TTCAAGGACGTGGAGTCGGACTCTGCCAAGCAGTTTTTGCAGGCAGCAGAGGCCATCGAT
GACATACCATTTGGGATCACTTCCAACAGTGACGTGTTCTCCAAATACCAGCTCGACAAA
GATGGGGTTGTCCTCTTTAAGAAGTTTGATGAAGGCCGGAACAACTTTGAAGGGGAGGTC
ACCAAGGAGAACCTGCTGGACTTTATCAAACACAACCAGCTGCCCCTTGTCATCGAGTTC
ACCGAGCAGACAGCCCCGAAGATTTTTGGAGGTGAAATCAAGACTCACATCCTGCTGTTC
TTGCCCAAGAGTGTGTCTGACTATGACGGCAAACTGAGCAACTTCAAAACAGCAGCCGAG
AGCTTCAAGGGCAAGATCCTGTTCATCTTCATCGACAGCGACCACACCGACAACCAGCGC
ATCCTCGAGTTCTTTGGCCTGAAGAAGGAAGAGTGCCCGGCCGTGCGCCTCATCACCCTG
GAGGAGGAGATGACCAAGTACAAGCCCGAATCGGAGGAGCTGACGGCAGAGAGGATCACA
GAGTTCTGCCACCGCTTCCTGGAGGGCAAAATCAAGCCCCACCTGATGAGCCAGGAGCTG
CCGGAGGACTGGGACAAGCAGCCTGTCAAGGTGCTTGTTGGGAAGAACTTTGAAGACGTG
GCTTTTGATGAGAAAAAAAACGTCTTTGTGGAGTTCTATGCCCCATGGTGTGGTCACTGC
AAACAGTTGGCTCCCATTTGGGATAAACTGGGAGAGACGTACAAGGACCATGAGAACATC
GTCATCGCCAAGATGGACTCGACTGCCAACGAGGTGGAGGCCGTCAAAGTGCACAGCTTC
CCCACACTCAAGTTCTTTCCTGCCAGTGCCGACAGGACGGTCATTGATTACAACGGGGAA
CGCACGCTGGATGGTTTTAAGAAATTCCTGGAGAGCGGTGGCCAGGATGGGGCAGGGGAT
GATGACGATCTCGAGGACCTGGAAGAAGCAGAGGAGCCAGACATGGAGGAAGACGATGAT
CAGAAAGCTGTGAAAGATGAACTGTAA

Clone variation with respect to NM_000918.3
>OriGene 5' read for NM_000918 unedited
GGCCGTTCCAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGCCGGG
ACCGGCGCGCGCTCTCGTCGCCCCCGCTGTCCCGGCGGCGCCAACCGAAGCGCCCCGCCT
GATCCGTGTCCGACATGCTGCGCCGCGCTCTGCTGTGCCTGGCCGTGGCCGCCCTGGTGC
GCGCCGACGCCCCCGAGGAGGAGGACCACGTCCTGGTGCTGCGGAAAAGCAACTTCGCGG
AGGCGCTGGCGGCCCACAAGTACCTGCTGGTGGAGTTCTATGCCCCTTGGTGTGGCCACT
GCAAGGCTCTGGCCCCTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCG
AGATCAGGTTGGCCAAGGTGGACGCCACGGAGGAGTCTGACCTGGCCCAGCAGTACGGCG
TGCGCGGCTATCCCACCATCAAGTTCTTCAGGAATGGAGACACGGCTTCCCCCAAGGAAT
ATACAGCTGGCAGAGAGGCTGATGACATCGTGAACTGGCTGAAGAAGCGCACGGGCCCGG
CTGCCACCACCCTGCCTGACGGCGCAGCTGCAGAGTCCTTGGTGGAGTCCAGCGAGGTGG
CTGTCATCGGCTTCTTCAAGGACGTGGAGTCGGACTCTGCCAAGCAGTTTTTGCAGGCAG
CAGAGGCCATCGATGACATACCATTTGGGATCACTTCCAACAGTGACGTGTTCTCCAAAT
ACCAGCTCGACAAGATGGNGTTGTCCTCTTTAAGAAGTTTGATGAAGGCCGGAACACTTT
GAAGGGGAGGTCACCAAGNAGAACCTGCTGGACTTTATCAAACACAACCAGCTGCCCCTT
GTCATCGAGTTCACCGAGCAGACAGCCCCCGAGATTTTTGGAGGTGAAATCAGACTCCAT
CN
>OriGene 3' read for NM_000918 unedited
TANNGATCGATTTTTTTTTTTTTTTTTTGGTGGTAAAATTTCAACTTTATTTGGCCAATG
TGTTCAATTCGATTGTGAAATAGAAATGCCTGAAGAACTGTCAGCGTCTGATTCAGCTCC
AGCATCCTTGGCCACCTCCCCACCCGGGAGTCAAGGGTCGTGGTTCTGCCTTGAACAGGC
CACAGGCCGTGCTGTAGAGAGGCCAGTGGTCACAATGAGCCCACGACAGGAGGAGGAGCC
CTGGCTTGAGGGAAGGGGAAGGCCCAGGCCTGTGCCGGGCCCCAGGGCTGGCAGCCACCA
GCTCCTCTTCCAGGCATGGGGGACACCCTGACAGGATCCGGAAGTCTCCATTTACCCAAA
AATGCAAGAGCCATGATCAGTCATGGCGACACTGCAGGCGGTACTGAGTGACCATGTCCA
GTCCGGCTCCGTCCCTCCCACACGGGGGACAAGCTTCTTCGAGGAGGCCTGGCCAAGGTG
GAACAAATACCCTGATGTCGAAAAATGTCTTAAAATCCCACAGACCGGATTTTCAAAAAG
AGGAGAAACCTCCCGCGGGAAGGAGGCAGCGAGACTCCCAACACGGGAGCCAGCACTCTG
GACAGACTCCTGTGTGAAAAAGTACATCATGGTTTTCAGGAAACAACCCCACCCAGGTGG
GGCTGCCCTGAAAAGGATCCCTTTCCAAAAACCCCAAAACACAAAGGAAAAACCGGGGTG
AACCGACCGTGTGTAGGGGGGAAGTGGTCCCTTCCAAGAAGTTCCCTGGGGTTTCCGGGG
AACCACCTCTCTTTAACAGAGGCCCCAGGTCTTTGAGGGCTGCGCCTCTG
Restriction Sites NotI-NotI     
ACCN NM_000918
Insert Size 2700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000918.2, NP_000909.2
RefSeq Size 2438 bp
RefSeq ORF 1527 bp
Locus ID 5034
Cytogenetics 17q25.3
Domains thiored
Protein Families Druggable Genome
Gene Summary 'This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]'

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