P4HB (NM_000918) Human Untagged Clone

CAT#: SC119620

P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P4HB
• Synonyms: CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119620 sequence for NM_000918 edited (data generated by NextGen Sequencing)
  ATGCTGCGCCGCGCTCTGCTGTGCCTGGCCGTGGCCGCCCTGGTGCGCGCCGACGCCCCC
  GAGGAGGAGGACCACGTCCTGGTGCTGCGGAAAAGCAACTTCGCGGAGGCGCTGGCGGCC
  CACAAGTACCTGCTGGTGGAGTTCTATGCCCCTTGGTGTGGCCACTGCAAGGCTCTGGCC
  CCTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATCAGGTTGGCC
  AAGGTGGACGCCACGGAGGAGTCTGACCTGGCCCAGCAGTACGGCGTGCGCGGCTATCCC
  ACCATCAAGTTCTTCAGGAATGGAGACACGGCTTCCCCCAAGGAATATACAGCTGGCAGA
  GAGGCTGATGACATCGTGAACTGGCTGAAGAAGCGCACGGGCCCGGCTGCCACCACCCTG
  CCTGACGGCGCAGCTGCAGAGTCCTTGGTGGAGTCCAGCGAGGTGGCTGTCATCGGCTTC
  TTCAAGGACGTGGAGTCGGACTCTGCCAAGCAGTTTTTGCAGGCAGCAGAGGCCATCGAT
  GACATACCATTTGGGATCACTTCCAACAGTGACGTGTTCTCCAAATACCAGCTCGACAAA
  GATGGGGTTGTCCTCTTTAAGAAGTTTGATGAAGGCCGGAACAACTTTGAAGGGGAGGTC
  ACCAAGGAGAACCTGCTGGACTTTATCAAACACAACCAGCTGCCCCTTGTCATCGAGTTC
  ACCGAGCAGACAGCCCCGAAGATTTTTGGAGGTGAAATCAAGACTCACATCCTGCTGTTC
  TTGCCCAAGAGTGTGTCTGACTATGACGGCAAACTGAGCAACTTCAAAACAGCAGCCGAG
  AGCTTCAAGGGCAAGATCCTGTTCATCTTCATCGACAGCGACCACACCGACAACCAGCGC
  ATCCTCGAGTTCTTTGGCCTGAAGAAGGAAGAGTGCCCGGCCGTGCGCCTCATCACCCTG
  GAGGAGGAGATGACCAAGTACAAGCCCGAATCGGAGGAGCTGACGGCAGAGAGGATCACA
  GAGTTCTGCCACCGCTTCCTGGAGGGCAAAATCAAGCCCCACCTGATGAGCCAGGAGCTG
  CCGGAGGACTGGGACAAGCAGCCTGTCAAGGTGCTTGTTGGGAAGAACTTTGAAGACGTG
  GCTTTTGATGAGAAAAAAAACGTCTTTGTGGAGTTCTATGCCCCATGGTGTGGTCACTGC
  AAACAGTTGGCTCCCATTTGGGATAAACTGGGAGAGACGTACAAGGACCATGAGAACATC
  GTCATCGCCAAGATGGACTCGACTGCCAACGAGGTGGAGGCCGTCAAAGTGCACAGCTTC
  CCCACACTCAAGTTCTTTCCTGCCAGTGCCGACAGGACGGTCATTGATTACAACGGGGAA
  CGCACGCTGGATGGTTTTAAGAAATTCCTGGAGAGCGGTGGCCAGGATGGGGCAGGGGAT
  GATGACGATCTCGAGGACCTGGAAGAAGCAGAGGAGCCAGACATGGAGGAAGACGATGAT
  CAGAAAGCTGTGAAAGATGAACTGTAA
  
  Clone variation with respect to NM_000918.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000918 unedited
  GGCCGTTCCAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGCCGGG
  ACCGGCGCGCGCTCTCGTCGCCCCCGCTGTCCCGGCGGCGCCAACCGAAGCGCCCCGCCT
  GATCCGTGTCCGACATGCTGCGCCGCGCTCTGCTGTGCCTGGCCGTGGCCGCCCTGGTGC
  GCGCCGACGCCCCCGAGGAGGAGGACCACGTCCTGGTGCTGCGGAAAAGCAACTTCGCGG
  AGGCGCTGGCGGCCCACAAGTACCTGCTGGTGGAGTTCTATGCCCCTTGGTGTGGCCACT
  GCAAGGCTCTGGCCCCTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCG
  AGATCAGGTTGGCCAAGGTGGACGCCACGGAGGAGTCTGACCTGGCCCAGCAGTACGGCG
  TGCGCGGCTATCCCACCATCAAGTTCTTCAGGAATGGAGACACGGCTTCCCCCAAGGAAT
  ATACAGCTGGCAGAGAGGCTGATGACATCGTGAACTGGCTGAAGAAGCGCACGGGCCCGG
  CTGCCACCACCCTGCCTGACGGCGCAGCTGCAGAGTCCTTGGTGGAGTCCAGCGAGGTGG
  CTGTCATCGGCTTCTTCAAGGACGTGGAGTCGGACTCTGCCAAGCAGTTTTTGCAGGCAG
  CAGAGGCCATCGATGACATACCATTTGGGATCACTTCCAACAGTGACGTGTTCTCCAAAT
  ACCAGCTCGACAAGATGGNGTTGTCCTCTTTAAGAAGTTTGATGAAGGCCGGAACACTTT
  GAAGGGGAGGTCACCAAGNAGAACCTGCTGGACTTTATCAAACACAACCAGCTGCCCCTT
  GTCATCGAGTTCACCGAGCAGACAGCCCCCGAGATTTTTGGAGGTGAAATCAGACTCCAT
  CN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000918 unedited
  TANNGATCGATTTTTTTTTTTTTTTTTTGGTGGTAAAATTTCAACTTTATTTGGCCAATG
  TGTTCAATTCGATTGTGAAATAGAAATGCCTGAAGAACTGTCAGCGTCTGATTCAGCTCC
  AGCATCCTTGGCCACCTCCCCACCCGGGAGTCAAGGGTCGTGGTTCTGCCTTGAACAGGC
  CACAGGCCGTGCTGTAGAGAGGCCAGTGGTCACAATGAGCCCACGACAGGAGGAGGAGCC
  CTGGCTTGAGGGAAGGGGAAGGCCCAGGCCTGTGCCGGGCCCCAGGGCTGGCAGCCACCA
  GCTCCTCTTCCAGGCATGGGGGACACCCTGACAGGATCCGGAAGTCTCCATTTACCCAAA
  AATGCAAGAGCCATGATCAGTCATGGCGACACTGCAGGCGGTACTGAGTGACCATGTCCA
  GTCCGGCTCCGTCCCTCCCACACGGGGGACAAGCTTCTTCGAGGAGGCCTGGCCAAGGTG
  GAACAAATACCCTGATGTCGAAAAATGTCTTAAAATCCCACAGACCGGATTTTCAAAAAG
  AGGAGAAACCTCCCGCGGGAAGGAGGCAGCGAGACTCCCAACACGGGAGCCAGCACTCTG
  GACAGACTCCTGTGTGAAAAAGTACATCATGGTTTTCAGGAAACAACCCCACCCAGGTGG
  GGCTGCCCTGAAAAGGATCCCTTTCCAAAAACCCCAAAACACAAAGGAAAAACCGGGGTG
  AACCGACCGTGTGTAGGGGGGAAGTGGTCCCTTCCAAGAAGTTCCCTGGGGTTTCCGGGG
  AACCACCTCTCTTTAACAGAGGCCCCAGGTCTTTGAGGGCTGCGCCTCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000918
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000918.2, NP_000909.2
• RefSeq Size: 2438 bp
• RefSeq ORF: 1527 bp
• Locus ID: 5034
• Cytogenetics: 17q25.3
• Domains: thiored
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]'