P4HB (NM_000918) Human Untagged Clone

CAT#: SC322507

P4HB (untagged)-Human prolyl 4-hydroxylase, beta polypeptide (P4HB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P4HB
• Synonyms: CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322507 CCCGGCGGCGCCAACCGAAGCGCCCCGCCTGATCCGTGTCCGACATGCTGCGCCGCGCTC
  TGCTGTGCCTGGCCGTGGCCGCCCTGGTGCGCGCCGACGCCCCCGAGGAGGAGGACCACG
  TCCTGGTGCTGCGGAAAAGCAACTTCGCGGAGGCGCTGGCGGCCCACAAGTACCTGCTGG
  TGGAGTTCTATGCCCCTTGGTGTGGCCACTGCAAGGCTCTGGCCCCTGAGTATGCCAAAG
  CCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATCAGGTTGGCCAAGGTGGACGCCACGG
  AGGAGTCTGACCTGGCCCAGCAGTACGGCGTGCGCGGCTATCCCACCATCAAGTTCTTCA
  GGAATGGAGACACGGCTTCCCCCAAGGAATATACAGCTGGCAGAGAGGCTGATGACATCG
  TGAACTGGCTGAAGAAGCGCACGGGCCCGGCTGCCACCACCCTGCCTGACGGCGCAGCTG
  CAGAGTCCTTGGTGGAGTCCAGCGAGGTGGCTGTCATCGGCTTCTTCAAGGACGTGGAGT
  CGGACTCTGCCAAGCAGTTTTTGCAGGCAGCAGAGGCCATCGATGACATACCATTTGGGA
  TCACTTCCAACAGTGACGTGTTCTCCAAATACCAGCTCGACAAAGATGGGGTTGTCCTCT
  TTAAGAAGTTTGATGAAGGCCGGAACAACTTTGAAGGGGAGGTCACCAAGGAGAACCTGC
  TGGACTTTATCAAACACAACCAGCTGCCCCTTGTCATCGAGTTCACCGAGCAGACAGCCC
  CGAAGATTTTTGGAGGTGAAATCAAGACTCACATCCTGCTGTTCTTGCCCAAGAGTGTGT
  CTGACTATGACGGCAAACTGAGCAACTTCAAAACAGCAGCCGAGAGCTTCAAGGGCAAGA
  TCCTGTTCATCTTCATCGACAGCGACCACACCGACAACCAGCGCATCCTCGAGTTCTTTG
  GCCTGAAGAAGGAAGAGTGCCCGGCCGTGCGCCTCATCACCCTGGAGGAGGAGATGACCA
  AGTACAAGCCCGAATCGGAGGAGCTGACGGCAGAGAGGATCACAGAGTTCTGCCACCGCT
  TCCTGGAGGGCAAAATCAAGCCCCACCTGATGAGCCAGGAGCTGCCGGAGGACTGGGACA
  AGCAGCCTGTCAAGGTGCTTGTTGGGAAGAACTTTGAAGACGTGGCTTTTGATGAGAAAA
  AAAACGTCTTTGTGGAGTTCTATGCCCCATGGTGTGGTCACTGCAAACAGTTGGCTCCCA
  TTTGGGATAAACTGGGAGAGACGTACAAGGACCATGAGAACATCGTCATCGCCAAGATGG
  ACTCGACTGCCAACGAGGTGGAGGCCGTCAAAGTGCACAGCTTCCCCACACTCAAGTTCT
  TTCCTGCCAGTGCCGACAGGACGGTCATTGATTACAACGGGGAACGCACGCTGGATGGTT
  TTAAGAAATTCCTGGAGAGCGGTGGCCAGGATGGGGCAGGGGATGATGACGATCTCGAGG
  ACCTGGAAGAAGCAGAGGAGCCAGACATGGAGGAAGACGATGATCAGAAAGCTGTGAAAG
  ATGAACTGTAATACGCAAAGCCAGACCCGGGCGCTGCCGAGACCCCTCGGGGGCTGCACA
  CCCAGCAGCAGCGCACGCCTCCGAAGCCTGCGGCCTCGCTTGAAGGAGGGCGTCGCCGGA
  AACCCAGGGAACCTCTCTGAAGTGACACCTCACCCCTACACACCGTCCGTTCACCCCCGT
  CTCTTCCTTCTGCTTTTCGGTTTTTGGAAAGGGATCCATCTCCAGGCAGCCCACCCTGGT
  GGGGCTTGTTTCCTGAAACCATGATGTACTTTTTCATACATGAGTCTGTCCAGAGTGCTT
  GCTACCGTGTTCGGAGTCTCGCTGCCTCCCTCCCGCGGGAGGTTTCTCCTCTTTTTGAAA
  ATTCCGTCTGTGGGATTTTTAGACATTTTTCGACATCAGGGTATTTGTTCCACCTTGGCC
  AGGCCTCCTCGGAGAAGCTTGTCCCCCGTGTGGGAGGGACGGAGCCGGACTGGACATGGT
  CACTCAGTACCGCCTGCAGTGTCGCCATGACTGATCATGGCTCTTGCATTTTTGGGTAAA
  TGGAGACTTCCGGATCCTGTCAGGGTGTCCCCCATGCCTGGAAGAGGAGCTGGTGGCTGC
  CAGCCCTGGGGCCCGGCACAGGCCTGGGCCTTCCCCTTCCCTCAAGCCAGGGCTCCTCCT
  CCTGTCGTGGGCTCATTGTGACCACTGGCCTCTCTACAGCACGGCCTGTGGCCTGTTCAA
  GGCAGAACCACGACCCTTGACTCCCGGGTGGGGAGGTGGCCAAGGATGCTGGAGCTGAAT
  CAGACGCTGACAGTTCTTCAGGCATTTCTATTTCACAATCGAATTGAACACATTGGCCAA
  ATAAAGTTGAAATTTTACCACCAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000918
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000918.3, NP_000909.2
• RefSeq Size: 2596 bp
• RefSeq ORF: 1527 bp
• Locus ID: 5034
• Cytogenetics: 17q25.3
• Domains: thiored
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]'