Fas Ligand (FASLG) (NM_000639) Human Untagged Clone

CAT#: SC119739

FASLG (untagged)-Human Fas ligand (TNF superfamily, member 6) (FASLG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FASLG
• Synonyms: ALPS1B; APT1LG1; APTL; CD95-L; CD95L; CD178; FASL; TNFSF6; TNLG1A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119739 sequence for NM_000639 edited (data generated by NextGen Sequencing)
  ATGCAGCAGCCCTTCAATTACCCATATCCCCAGATCTACTGGGTGGACAGCAGTGCCAGC
  TCTCCCTGGGCCCCTCCAGGCACAGTTCTTCCCTGTCCAACCTCTGTGCCCAGAAGGCCT
  GGTCAAAGGAGGCCACCACCACCACCGCCACCGCCACCACTACCACCTCCGCCGCCGCCG
  CCACCACTGCCTCCACTACCGCTGCCACCCCTGAAGAAGAGAGGGAACCACAGCACAGGC
  CTGTGTCTCCTTGTGATGTTTTTCATGGTTCTGGTTGCCTTGGTAGGATTGGGCCTGGGG
  ATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGGCAGAACTCCGAGAGTCTACCAGCCAG
  ATGCACACAGCATCATCTTTGGAGAAGCAAATAGGCCACCCCAGTCCACCCCCTGAAAAA
  AAGGAGCTGAGGAAAGTGGCCCATTTAACAGGCAAGTCCAACTCAAGGTCCATGCCTCTG
  GAATGGGAAGACACCTATGGAATTGTCCTGCTTTCTGGAGTGAAGTATAAGAAGGGTGGC
  CTTGTGATCAATGAAACTGGGCTGTACTTTGTATATTCCAAAGTATACTTCCGGGGTCAA
  TCTTGCAACAACCTGCCCCTGAGCCACAAGGTCTACATGAGGAACTCTAAGTATCCCCAG
  GATCTGGTGATGATGGAGGGGAAGATGATGAGCTACTGCACTACTGGGCAGATGTGGGCC
  CGCAGCAGCTACCTGGGGGCAGTGTTCAATCTTACCAGTGCTGATCATTTATATGTCAAC
  GTATCTGAGCTCTCTCTGGTCAATTTTGAGGAATCTCAGACGTTTTTCGGCTTATATAAG
  CTCTAA
  
  Clone variation with respect to NM_000639.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000639 unedited
  TAAGTCATATTTGTATACGACTCATATAGGCGGCCGACGAATTCGCACCAGGTCCTTGAC
  ACCTCAGCCTCTAAGGACTGAGAAGAAGTAAAACCGTTTGCTGGGGCTGGCCTGACTCAC
  CAGCTGCCATGCAGCAGCCCTTCAATTACCCATATCCCCAGATCTACTGGGTGGACAGCA
  GTGCCAGCTCTCCCTGGGCCCCTCCAGGCACAGTTCTTCCCTGTCCAACCTCTGTGCCCA
  GAAGGCCTGGTCAAAGGAGGCCACCACCACCACCGCCACCGCCACCACTACCACCTCCGC
  CGCCGCCGCCACCACTGCCTCCACTACCGCTGCCACCCCTGAAGAAGAGAGGGAACCACA
  GCACAGGCCTGTGTCTCCTTGTGATGTTTTTCATGGTTCTGGTTGCCTTGGTAGGATTGG
  GCCTGGGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGGCAGAACTCCGAGAGTCTA
  CCAGCCAGATGCACACAGCATCATCTTTGGAGAAGCAAATAGGCCACCCCAGTCCACCCC
  CTGAAAAAAAGGAGCTGAGGAAAGTGGCCCATTTAACAGGCAAGTCCAACTCAAGGTCCA
  TGCCTCTGGAATGGGAAGACACCTATGGAATTGTCCTGCTTTCTGGAGTGAAGTATAAGA
  AGGGTGGCCTTGTGATCAATGAAACTGGGCTGTACTTTGTATATTCCAAAGTATACTTCC
  GGAGTCAATCTTGCAACAACCTGCCCCTGAGCCACAAGGTCTACATGATGAAACTCTAGT
  ANTCCCAGGATCTGGTGATGATGGAGGGGGAAGATGATGAGCTACTGCACTACTGGGCAG
  ATGTGGGCCCGCAGCAGCTACCTGGGGGCAGTGGTCAATCTTACCCAGTGCTGATCATTA
  TATGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000639 unedited
  NAAANTTTAGCTTTGGACCCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTGGGACCCAAACATAAACTTTTTATTAC
  AGGTTTTCATTTCACAAAAAGGCCCATCTCCCTAAATTTTTATTATTTTATAGGAAAACA
  TCTTTAGTAGCTGACAATCAAGGATTTTTAAGCCCTTTTAAAGTTGATTGTCAGGAACCA
  TTCAAAATTTTGACCAAAGGCAACCCATATTTTCCTAATATGGCCTTCACAATATCTACA
  TTCTTTTTTTAGTCATACACACACACACCCACACACCCCCACACCCCCCCTTACAATGGG
  ACTGGAAATACCCATGCTGGGTTAGGAATGAAATGAGTCCCCAAAACATTTCTTTTGCTT
  ATCTGGAAGTTCCACCGAGGGCTCAGGATTTGCTTTTAAAAGGGAAAATGCTGCCAGGGG
  GGCATTGGCCTTTTTCACCCTTTTTAGCTTATTATGCAAGCCTCTAGTCTTCCTTTTCCA
  TCCCTTAAATCCTCAGGGCACAACCATGTTTCTGAAAAACAGCCCCCCTTGAGGTAGCAA
  GGCCCACCTTGAGAGGGGAGTTAAAAGGGGTCCTTTTCAATCTGCCTAAATACTCTCAGG
  CCCTAAGATGAGGTGAAAAACACCTGGGANCGGGTTTTTAATGCTTCCCCCCTCTTAACA
  GGGGAGCCCAAGAGTTCTATGGTCCTCCGCACATATCCCCCCCTTTGGGGGGGCGTCATA
  ACCCTTAGGGGGAAGTTGGAGGGAGCTCCAAAACATTTGAACCCCCGTGGGGTCCAAAGG
  GGCCACTTGGGTTCTAGGTTTTTTTACCCTGGCCCCAAAAGGCGTTGAAAAAAACCCTTC
  CCTGGAAACACAATTTCTGGGGGCTGGTAAACAAAAATTTATAGGGAAGAATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000639
• Insert Size: 1790 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000639.1, NP_000630.1
• RefSeq Size: 1909 bp
• RefSeq ORF: 846 bp
• Locus ID: 356
• Cytogenetics: 1q24.3
• Domains: TNF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Apoptosis, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathways in cancer, Type I diabetes mellitus
• Gene Summary: 'This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).