Fas Ligand (FASLG) (NM_000639) Human Untagged Clone

CAT#: SC119739

FASLG (untagged)-Human Fas ligand (TNF superfamily, member 6) (FASLG)


  "NM_000639" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol FASLG
Synonyms ALPS1B; APT1LG1; APTL; CD95-L; CD95L; CD178; FASL; TNFSF6; TNLG1A
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119739 sequence for NM_000639 edited (data generated by NextGen Sequencing)
ATGCAGCAGCCCTTCAATTACCCATATCCCCAGATCTACTGGGTGGACAGCAGTGCCAGC
TCTCCCTGGGCCCCTCCAGGCACAGTTCTTCCCTGTCCAACCTCTGTGCCCAGAAGGCCT
GGTCAAAGGAGGCCACCACCACCACCGCCACCGCCACCACTACCACCTCCGCCGCCGCCG
CCACCACTGCCTCCACTACCGCTGCCACCCCTGAAGAAGAGAGGGAACCACAGCACAGGC
CTGTGTCTCCTTGTGATGTTTTTCATGGTTCTGGTTGCCTTGGTAGGATTGGGCCTGGGG
ATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGGCAGAACTCCGAGAGTCTACCAGCCAG
ATGCACACAGCATCATCTTTGGAGAAGCAAATAGGCCACCCCAGTCCACCCCCTGAAAAA
AAGGAGCTGAGGAAAGTGGCCCATTTAACAGGCAAGTCCAACTCAAGGTCCATGCCTCTG
GAATGGGAAGACACCTATGGAATTGTCCTGCTTTCTGGAGTGAAGTATAAGAAGGGTGGC
CTTGTGATCAATGAAACTGGGCTGTACTTTGTATATTCCAAAGTATACTTCCGGGGTCAA
TCTTGCAACAACCTGCCCCTGAGCCACAAGGTCTACATGAGGAACTCTAAGTATCCCCAG
GATCTGGTGATGATGGAGGGGAAGATGATGAGCTACTGCACTACTGGGCAGATGTGGGCC
CGCAGCAGCTACCTGGGGGCAGTGTTCAATCTTACCAGTGCTGATCATTTATATGTCAAC
GTATCTGAGCTCTCTCTGGTCAATTTTGAGGAATCTCAGACGTTTTTCGGCTTATATAAG
CTCTAA

Clone variation with respect to NM_000639.1
>OriGene 5' read for NM_000639 unedited
TAAGTCATATTTGTATACGACTCATATAGGCGGCCGACGAATTCGCACCAGGTCCTTGAC
ACCTCAGCCTCTAAGGACTGAGAAGAAGTAAAACCGTTTGCTGGGGCTGGCCTGACTCAC
CAGCTGCCATGCAGCAGCCCTTCAATTACCCATATCCCCAGATCTACTGGGTGGACAGCA
GTGCCAGCTCTCCCTGGGCCCCTCCAGGCACAGTTCTTCCCTGTCCAACCTCTGTGCCCA
GAAGGCCTGGTCAAAGGAGGCCACCACCACCACCGCCACCGCCACCACTACCACCTCCGC
CGCCGCCGCCACCACTGCCTCCACTACCGCTGCCACCCCTGAAGAAGAGAGGGAACCACA
GCACAGGCCTGTGTCTCCTTGTGATGTTTTTCATGGTTCTGGTTGCCTTGGTAGGATTGG
GCCTGGGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGGCAGAACTCCGAGAGTCTA
CCAGCCAGATGCACACAGCATCATCTTTGGAGAAGCAAATAGGCCACCCCAGTCCACCCC
CTGAAAAAAAGGAGCTGAGGAAAGTGGCCCATTTAACAGGCAAGTCCAACTCAAGGTCCA
TGCCTCTGGAATGGGAAGACACCTATGGAATTGTCCTGCTTTCTGGAGTGAAGTATAAGA
AGGGTGGCCTTGTGATCAATGAAACTGGGCTGTACTTTGTATATTCCAAAGTATACTTCC
GGAGTCAATCTTGCAACAACCTGCCCCTGAGCCACAAGGTCTACATGATGAAACTCTAGT
ANTCCCAGGATCTGGTGATGATGGAGGGGGAAGATGATGAGCTACTGCACTACTGGGCAG
ATGTGGGCCCGCAGCAGCTACCTGGGGGCAGTGGTCAATCTTACCCAGTGCTGATCATTA
TATGT
>OriGene 3' read for NM_000639 unedited
NAAANTTTAGCTTTGGACCCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTGGGACCCAAACATAAACTTTTTATTAC
AGGTTTTCATTTCACAAAAAGGCCCATCTCCCTAAATTTTTATTATTTTATAGGAAAACA
TCTTTAGTAGCTGACAATCAAGGATTTTTAAGCCCTTTTAAAGTTGATTGTCAGGAACCA
TTCAAAATTTTGACCAAAGGCAACCCATATTTTCCTAATATGGCCTTCACAATATCTACA
TTCTTTTTTTAGTCATACACACACACACCCACACACCCCCACACCCCCCCTTACAATGGG
ACTGGAAATACCCATGCTGGGTTAGGAATGAAATGAGTCCCCAAAACATTTCTTTTGCTT
ATCTGGAAGTTCCACCGAGGGCTCAGGATTTGCTTTTAAAAGGGAAAATGCTGCCAGGGG
GGCATTGGCCTTTTTCACCCTTTTTAGCTTATTATGCAAGCCTCTAGTCTTCCTTTTCCA
TCCCTTAAATCCTCAGGGCACAACCATGTTTCTGAAAAACAGCCCCCCTTGAGGTAGCAA
GGCCCACCTTGAGAGGGGAGTTAAAAGGGGTCCTTTTCAATCTGCCTAAATACTCTCAGG
CCCTAAGATGAGGTGAAAAACACCTGGGANCGGGTTTTTAATGCTTCCCCCCTCTTAACA
GGGGAGCCCAAGAGTTCTATGGTCCTCCGCACATATCCCCCCCTTTGGGGGGGCGTCATA
ACCCTTAGGGGGAAGTTGGAGGGAGCTCCAAAACATTTGAACCCCCGTGGGGTCCAAAGG
GGCCACTTGGGTTCTAGGTTTTTTTACCCTGGCCCCAAAAGGCGTTGAAAAAAACCCTTC
CCTGGAAACACAATTTCTGGGGGCTGGTAAACAAAAATTTATAGGGAAGAATC
Restriction Sites NotI-NotI     
ACCN NM_000639
Insert Size 1790 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000639.1, NP_000630.1
RefSeq Size 1909 bp
RefSeq ORF 846 bp
Locus ID 356
Cytogenetics 1q24.3
Domains TNF
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways Allograft rejection, Apoptosis, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathways in cancer, Type I diabetes mellitus
Gene Summary 'This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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