Fas Ligand (FASLG) (NM_000639) Human Untagged Clone

CAT#: SC320927

FASLG (untagged)-Human Fas ligand (TNF superfamily, member 6) (FASLG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FASLG
• Synonyms: ALPS1B; APT1LG1; APTL; CD95-L; CD95L; CD178; FASL; TNFSF6; TNLG1A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000639.1 CAGGGTCCCGTCCTTGACACCTCAGCCTCTACAGGACTGAGAAGAAGTAAAACCGTTTGC
  TGGGGCTGGCCTGACTCACCAGCTGCCATGCAGCAGCCCTTCAATTACCCATATCCCCAG
  ATCTACTGGGTGGACAGCAGTGCCAGCTCTCCCTGGGCCCCTCCAGGCACAGTTCTTCCC
  TGTCCAACCTCTGTGCCCAGAAGGCCTGGTCAAAGGAGGCCACCACCACCACCGCCACCG
  CCACCACTACCACCTCCGCCGCCGCCGCCACCACTGCCTCCACTACCGCTGCCACCCCTG
  AAGAAGAGAGGGAACCACAGCACAGGCCTGTGTCTCCTTGTGATGTTTTTCATGGTTCTG
  GTTGCCTTGGTAGGATTGGGCCTGGGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTG
  GCAGAACTCCGAGAGTCTACCAGCCAGATGCACACAGCATCATCTTTGGAGAAGCAAATA
  GGCCACCCCAGTCCACCCCCTGAAAAAAAGGAGCTGAGGAAAGTGGCCCATTTAACAGGC
  AAGTCCAACTCAAGGTCCATGCCTCTGGAATGGGAAGACACCTATGGAATTGTCCTGCTT
  TCTGGAGTGAAGTATAAGAAGGGTGGCCTTGTGATCAATGAAACTGGGCTGTACTTTGTA
  TATTCCAAAGTATACTTCCGGGGTCAATCTTGCAACAACCTGCCCCTGAGCCACAAGGTC
  TACATGAGGAACTCTAAGTATCCCCAGGATCTGGTGATGATGGAGGGGAAGATGATGAGC
  TACTGCACTACTGGGCAGATGTGGGCCCGCAGCAGCTACCTGGGGGCAGTGTTCAATCTT
  ACCAGTGCTGATCATTTATATGTCAACGTATCTGAGCTCTCTCTGGTCAATTTTGAGGAA
  TCTCAGACGTTTTTCGGCTTATATAAGCTCTAAGAGAAGCACTTTGGGATTCTTTCCATT
  ATGATTCTTTGTTACAGGCACCGAGAATGTTGTATTCAGTGAGGGTCTTCTTACATGCAT
  TTGAGGTCAAGTAAGAAGACATGAACCAAGTGGACCTTGAGACCACAGGGTTCAAAATGT
  CTGTAGCTCCTCAACTCACCTAATGTTTATGAGCCAGACAAATGGAGGAATATGACGGAA
  GAACATAGAACTCTGGGCTGCCATGTGAAGAGGGAGAAGCATGAAAAAGCAGCTACCAGG
  TGTTCTACACTCATCTTAGTGCCTGAGAGTATTTAGGCAGATTGAAAAGGACACCTTTTA
  ACTCACCTCTCAAGGTGGGCCTTGCTACCTCAAGGGGGACTGTCTTTCAGATACATGGTT
  GTGACCTGAGGATTTAAGGGATGGAAAAGGAAGACTAGAGGCTTGCATAATAAGCTAAAG
  AGGCTGAAAGAGGCCAATGCCCCACTGGCAGCATCTTCACTTCTAAATGCATATCCTGAG
  CCATCGGTGAAACTAACAGATAAGCAAGAGAGATGTTTTGGGGACTCATTTCATTCCTAA
  CACAGCATGTGTATTTCCAGTGCAATTGTAGGGGTGTGTGTGTGTGTGTGTGTGTGTGTG
  TGTGTATGACTAAAGAGAGAATGTAGATATTGTGAAGTACATATTAGGAAAATATGGGTT
  GCATTTGGTCAAGATTTTGAATGCTTCCTGACAATCAACTCTAATAGTGCTTAAAAATCA
  TTGATTGTCAGCTACTAATGATGTTTTCCTATAATATAATAAATATTTATGTAGATGTGC
  ATTTTTGTGAAATGAAAACATGTAATAAAAAGTATATGTTAGGATACAAAAAAAAAAAAA
  AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000639
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000639.1, NP_000630.1
• RefSeq Size: 1909 bp
• RefSeq ORF: 846 bp
• Locus ID: 356
• Cytogenetics: 1q24.3
• Domains: TNF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Apoptosis, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathways in cancer, Type I diabetes mellitus
• Gene Summary: 'This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).