ADH5 (NM_000671) Human Untagged Clone

CAT#: SC119755

ADH5 (untagged)-Human alcohol dehydrogenase 5 (class III), chi polypeptide (ADH5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADH5
• Synonyms: ADH-3; ADHX; FALDH; FDH; GSH-FDH; GSNOR; HEL-S-60p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000671, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGAACGAGGTTATCAAGTGCAAGGCTGCAGTTGCTTGGGAGGCTGGAAAGCCTCTCTCCATAGAGG
  AGATAGAGGTGGCACCCCCAAAGGCTCATGAAGTTCGAATCAAGATCATTGCCACTGCGGTTTGCCACAC
  CGATGCCTATACCCTGAGTGGAGCTGATCCTGAGGGTTGTTTTCCAGTGATCTTGGGACATGAAGGTGCT
  GGAATTGTGGAAAGTGTTGGTGAGGGAGTTACTAAGCTGAAGGCGGGTGACACTGTCATCCCACTTTACA
  TCCCACAGTGTGGAGAATGCAAATTTTGTCTAAATCCTAAAACTAACCTTTGCCAGAAGATAAGAGTCAC
  TCAAGGGAAAGGATTAATGCCAGATGGTACCAGCAGATTTACTTGCAAAGGAAAGACAATTTTGCATTAC
  ATGGGAACCAGCACATTTTCTGAATACACAGTTGTGGCTGATATCTCTGTTGCTAAAATAGATCCTTTAG
  CACCTTTGGATAAAGTCTGCCTTCTAGGTTGTGGCATTTCAACCGGTTATGGTGCTGCTGTGAACACTGC
  CAAGTTGGAGCCTGGCTCTGTTTGTGCCGTCTTTGGTCTGGGAGGAGTCGGATTGGCAGTTATCATGGGC
  TGTAAAGTGGCTGGTGCTTCCCGGATCATTGGTGTGGACATCAATAAAGATAAATTTGCAAGGGCCAAAG
  AGTTTGGAGCCACTGAATGTATTAACCCTCAGGATTTTAGTAAACCCATCCAGGAAGTGCTCATTGAGAT
  GACCGATGGAGGAGTGGACTATTCCTTTGAATGTATTGGTAATGTGAAGGTCATGAGAGCAGCACTTGAG
  GCATGTCACAAGGGCTGGGGCGTCAGCGTCGTGGTTGGAGTAGCTGCTTCAGGTGAAGAAATTGCCACTC
  GTCCATTCCAGCTGGTAACAGGTCGCACATGGAAAGGCACTGCCTTTGGAGGATGGAAGAGTGTAGAAAG
  TGTCCCAAAGTTGGTGTCTGAATATATGTCCAAAAAGATAAAAGTTGATGAATTTGTGACTCACAATCTG
  TCTTTTGATGAAATCAACAAAGCCTTTGAACTGATGCATTCTGGAAAGAGCATTCGAACTGTTGTAAAGA
  TTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000671 unedited
  NGGTTCACTATTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCCAGAA
  TCCGTGAACATGGCGAACGAGGTTATCAAGTGCAAGGCTGCAGTTGCTTGGGAGGCTGGA
  AAGCCTCTCTCCATAGAGGAGATAGAGGTGGCACCCCCAAAGGCTCATGAAGTTCGAATC
  AAGATCATTGCCACTGCGGTTTGCCACACCGATGCCTATACCCTGAGTGGAGCTGATCCT
  GAGGGTTGTTTTCCAGTGATCTTGGGACATGAAGGTGCTGGAATTGTGGAAAGTGTTGGT
  GAGGGAGTTACTAAGCTGAAGGCGGGTGACACTGTCATCCCACTTTACATCCCACAGTGT
  GGAGAATGCAAATTTTGTCTAAATCCTAAAACTAACCTTTGCCAGAAGATAAGAGTCACT
  CAAGGGAAAGGATTAATGCCAGATGGTACCAGCAGATTTACTTGCAAAGGAAAGACAATT
  TTGCATTACATGGGAACCAGCACATTTTCTGAATACACAGTTGTGGCTGATATCTCTGTT
  GCTAAAATAGATCCTTTAGCACCTTTGGATAAAGTCTGCCTTCTAGGTTGTGGCATTTCA
  ACCGGTTATGGTGCTGCTGTGAACACTGCCAAGTTGGAGCCTGGCTCTGTTTGTGCCGTC
  TTTGGTCTGGGAGGAGTCGGATTGGCAGTTATCATGGGCTGTAAAGTGGCTGGTGCTTCC
  CGGATCATTGGTGTGGACATCAATAAAGATAAATTTGCAAGGGCCACAAGAGTTGGAGCC
  ACTGAATGTATTACCCCTCAGATTTTAGTAAACCCATCCAGGAAGTGCTCATTGAGATGA
  CCGATGGAGGAGTGGACTATTCCCTCGAATGTATTGGTAATGTGAAGGCATGAGNACCAC
  ACTTGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000671 unedited
  TTAAACTTTATTCCAACAAAATACCATAAAGTAAAAGAAAAGTCACAAAATAGACTAGAA
  GATATTTGCAACATATAAAACTGACAAATGATTGATGTCTAGAATAAAAGAACTCCTAAA
  AATTATTCAGAGAAAAATAATTCAATACAAAGTTGAGCACAGGATATGAACAGGTTCTTC
  ACAATAAAATGTCCATTAGAATGAAGGATTTAACGAGAATTGAGGAAAATGCAAACTAAA
  GTCATAATAAAACACCATTCCATACTCATGTAATTCACAAAAACTCACATTTACTTTGAA
  GAGCAATTTGGCAATGTCTATAAGGCTAAATGTGTTTATTCTGTGATACGTCTTAGGTAA
  CACGCAGTGAGCCCAATGCAGAAGAATGTTCCACTACAGTGTTATAATACAGAAACTGGC
  AACAACTTCATGCCTTCAACTGGAGAACAGATAAATTATGGTAGTTCCCGTAATTGAATT
  CTGCTACACAGGACTAAAATTAATGAACGAGAGCTTCAGATATAATTATACAATAATGAG
  TGAAAAAAGCTGTAGAATGAGCAAGTATGAGGCTTAAAAATATATAAAACAGTACTAAAG
  AGTAGATGGTTTAGGTTATACACAGTGAAAGTACAACAACACAGGAATGATAAACATCAA
  ATTGTGGATAATTGTTACCTCTGGACGCGAGGAATGGCAATGTGATCTCCAAGTGGCAAA
  ATGTTAAGATTTGACANAAGTACGTGCTGAGAAACTTGGATATTCAGTATGTAGTTCACT
  ATACTAGAAATAGGTCCAGTCCCACCAACACATACATACACCTATNTAAGCCCAAAAGCT
  GGTTCAATATACATTTTAATGCCCCCTGAAGGGCGATGCATCTAAGAATTTGGGGACCAG
  GTTAACAAGCCCAATCCTCCTTACAAAATTCCCAAACAAACTGGGCTCGATAATTTGACT
  GAGGGTGAATCGTTTTTAACACGGGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000671
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000671.3, NP_000662.3
• RefSeq Size: 2644 bp
• RefSeq ORF: 1125 bp
• Locus ID: 128
• Cytogenetics: 4q23
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Methane metabolism, Retinol metabolism, Tyrosine metabolism
• Gene Summary: 'This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]'