5 HT1A (HTR1A) (NM_000524) Human Untagged Clone

CAT#: SC119816

HTR1A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HTR1A
• Synonyms: 5-HT-1A; 5-HT1A; 5HT1a; ADRB2RL1; ADRBRL1; G-21; PFMCD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000524, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATGTGCTCAGCCCTGGTCAGGGCAACAACACCACATCACCACCGGCTCCCTTTGAGACCGGCGGCA
  ACACTACTGGTATCTCCGACGTGACCGTCAGCTACCAAGTGATCACCTCTCTGCTGCTGGGCACGCTCAT
  CTTCTGCGCGGTGCTGGGCAATGCGTGCGTGGTGGCTGCCATCGCCTTGGAGCGCTCCCTGCAGAACGTG
  GCCAATTATCTTATTGGCTCTTTGGCGGTCACCGACCTCATGGTGTCGGTGTTGGTGCTGCCCATGGCCG
  CGCTGTATCAGGTGCTCAACAAGTGGACACTGGGCCAGGTAACCTGCGACCTGTTCATCGCCCTCGACGT
  GCTGTGCTGCACCTCATCCATCTTGCACCTGTGCGCCATCGCGCTGGACAGGTACTGGGCCATCACGGAC
  CCCATCGACTACGTGAACAAGAGGACGCCCCGGCGCGCCGCTGCGCTCATCTCGCTCACTTGGCTTATTG
  GCTTCCTCATCTCTATCCCGCCCATGCTGGGCTGGCGCACCCCGGAAGACCGCTCGGACCCCGACGCATG
  CACCATTAGCAAGGATCATGGCTACACTATCTATTCCACCTTTGGAGCTTTCTACATCCCGCTGCTGCTC
  ATGCTGGTTCTCTATGGGCGCATATTCCGAGCTGCGCGCTTCCGCATCCGCAAGACGGTCAAAAAGGTGG
  AGAAGACCGGAGCGGACACCCGCCATGGAGCATCTCCCGCCCCGCAGCCCAAGAAGAGTGTGAATGGAGA
  GTCGGGGAGCAGGAACTGGAGGCTGGGCGTGGAGAGCAAGGCTGGGGGTGCTCTGTGCGCCAATGGCGCG
  GTGAGGCAAGGTGACGATGGCGCCGCCCTGGAGGTGATCGAGGTGCACCGAGTGGGCAACTCCAAAGAGC
  ACTTGCCTCTGCCCAGCGAGGCTGGTCCTACCCCTTGTGCCCCCGCCTCTTTCGAGAGGAAAAATGAGCG
  CAACGCCGAGGCGAAGCGCAAGATGGCCCTGGCCCGAGAGAGGAAGACAGTGAAGACGCTGGGCATCATC
  ATGGGCACCTTCATCCTCTGCTGGCTGCCCTTCTTCATCGTGGCTCTTGTTCTGCCCTTCTGCGAGAGCA
  GCTGCCACATGCCCACCCTGTTGGGCGCCATAATCAATTGGCTGGGCTACTCCAACTCTCTGCTTAACCC
  CGTCATTTACGCATACTTCAACAAGGACTTTCAAAACGCGTTTAAGAAGATCATTAAGTGTAAGTTCTGC
  CGCCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000524 unedited
  ATTTTGTAATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGCAGCTCCGGCAG
  CCAGTTCGGGAGCGGCAAAGTAAAATGGACAGCGACAGACAGACGTTCCAGCCACCTCTC
  CGCCGCCGGGAGATCCTGGAGCTGCTTTCAGGCCAACTCCAGTTTCCCAGCTGGAGCTTC
  TGAACGCGCTGGACTGCGAGAGCCCAGGGAGCGCCTGAAAGCTGCTCCTCGGAGATACCC
  TTCGCCGAAGCAGTAAGAACTTCCTGCTTGGGTCTCTGCATTCCCTTCCTCCGAAACTTC
  CCAGGAGAAGGGCGGAAGACCCCAGGGGAAGGGGCGAGGCGAATCTTCGCGCTGCTTTTT
  CTTCCCTCCCCCTTCCCGCGCCGGGCGCGCAGGCATGGATGTGCTCAGCCCTGGTCAGGG
  CAACAACACCACATCACCACCGGCTCCCTTTGAGACCGGCGGCAACACTACTGGTATCTC
  CGACGTGACCGTCAGCTACCAAGTGATCACCTCTCTGCTGCTGGGCACGCTCATCTTCTG
  CGCGGTGCTGGGCAATGCGTGCGTGGTGGCTGCCATCGCCTTGGAGCGCTCCCTGCAGAA
  CGTGGCCAATTATCTTATTGGCTCTTTGGCGGTCACCGACCTCATGGTGGTCGGTGTTGG
  GTGCTGCCCATGGCCGCGCTGTATCAGGTGCTCAACAAGTGGACACTGGGCCAGGTAACC
  TGCGACCTNGTCATCGCCCTCGACGTGCTGTGCTGCACCTCATCCATCTTGCACCTGTGG
  CGCATCGCGCTGGACAGGTACTGGGCCATCACGGACCCCATCGACTACGTGAACAGAGGA
  CGCCCGGGCGCCGCGNTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000524 unedited
  AATCNTGNCCGCGGCCGCATCTANAGTCGGTTTTTTTTTTTTTTTTTTTTTTTTTGAGAA
  ATGCAGCTATTTTATTTCTCTAAATTCTCTATTGCCTTGACAATTATGCTCCAGTTGGAT
  GTTCCTTCTCCATTCTTGGGTAAATATTCCAAAGCCGAAAGAACAAATGTCCAAAAGTCA
  TTGTCATACAATTCTGTAAAAGACACAAATAAATGGAAGTCTCAAAACTTCTAATGTAAA
  ACTTGCTGGCCAAGACATAATTTGGATGAGCAAATTTGGGGTTTGGATTATTTTAAATAT
  AGACTTTGTTCTTAAAATTTAGTGTAACATACAGAAAAACTTGGTTTCTGATATGGCTTT
  ACTTAGATTATGTGAGCAAAAATGAAACTGAATTATCAAATGAGTAATAGTTGTGCTGGA
  GAAGTCTATCTTATCTTCTTCACTGCAGACCCTTCTCAGCATTATACATGCATAGACAAA
  CTATTCCCAAAGTTCTAAAACTTTCCCTGTTTAGCTGGGATATGATGTCTACTTCCAGGT
  TAGAACACAGAAGCCCACACCCAAGGAAAGCTTTGTTCAATCTGACAAACAATAAAATGG
  CTTTAACTGACAGAGCTGGCCTTTCACTTTTTTAAATATAAAGAAAGAACTTCTCACTGG
  CAATTTTTGCAACCCGTAAACGCTATCCGGTTTTTCAAACCACCCAATGAAACACTTCCA
  AAGGTCCCCGTTTGACCACAACAAGAATCCGGGACACATTACTTTGGGAAATTTTTGGGA
  AAACAAGACCATTATTTATAAGAAAAATGGCGGGTTCAATTAAACACAGGCCAACCTTCT
  CATCCCCCTCCCGCGGCTTGGGCCAACCACCCTCCCGGGTACCCTACTAATTTTTCTCTT
  TTGTGGGTAAAAAGACTTCACCACCTCCCCCCGCCAAGGATGCCCACGGCTCCCCAAACA
  CCCGTAAAGGAGAAAACCCCGGCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000524
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000524.2, NP_000515.2
• RefSeq Size: 1269 bp
• RefSeq ORF: 1269 bp
• Locus ID: 3350
• Cytogenetics: 5q12.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]'