PMS2 (NM_000535) Human Untagged Clone

CAT#: SC119825

PMS2 (untagged)-Human PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMS2
• Synonyms: HNPCC4; MLH4; PMS2CL; PMSL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000535, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCGAGCTGAGAGCTCGAGTACAGAACCTGCTAAGGCCATCAAACCTATTGATCGGAAGTCAGTCC
  ATCAGATTTGCTCTGGGCAGGTGGTACTGAGTCTAAGCACTGCGGTAAAGGAGTTAGTAGAAAACAGTCT
  GGATGCTGGTGCCACTAATATTGATCTAAAGCTTAAGGACTATGGAGTGGATCTTATTGAAGTTTCAGAC
  AATGGATGTGGGGTAGAAGAAGAAAACTTCGAAGGCTTAACTCTGAAACATCACACATCTAAGATTCAAG
  AGTTTGCCGACCTAACTCAGGTTGAAACTTTTGGCTTTCGGGGGGAAGCTCTGAGCTCACTTTGTGCACT
  GAGCGATGTCACCATTTCTACCTGCCACGCATCGGCGAAGGTTGGAACTCGACTGATGTTTGATCACAAT
  GGGAAAATTATCCAGAAAACCCCCTACCCCCGCCCCAGAGGGACCACAGTCAGCGTGCAGCAGTTATTTT
  CCACACTACCTGTGCGCCATAAGGAATTTCAAAGGAATATTAAGAAGGAGTATGCCAAAATGGTCCAGGT
  CTTACATGCATACTGTATCATTTCAGCAGGCATCCGTGTAAGTTGCACCAATCAGCTTGGACAAGGAAAA
  CGACAGCCTGTGGTATGCACAGGTGGAAGCCCCAGCATAAAGGAAAATATCGGCTCTGTGTTTGGGCAGA
  AGCAGTTGCAAAGCCTCATTCCTTTTGTTCAGCTGCCCCCTAGTGACTCCGTGTGTGAAGAGTACGGTTT
  GAGCTGTTCCGATGCTCTGCATAATCTTTTTTACATCTCAGGTTTCATTTCACAATGCACGCATGGAGTT
  GGAAGGAGTTCAACAGACAGACAGTTTTTCTTTATCAACCGGCGGCCTTGTGACCCAGCAAAGGTCTGCA
  GACTCGTGAATGAGGTCTACCACATGTATAATCGACACCAGTATCCATTTGTTGTTCTTAACATTTCTGT
  TGATTCAGAATGCGTTGATATCAATGTTACTCCAGATAAAAGGCAAATTTTGCTACAAGAGGAAAAGCTT
  TTGTTGGCAGTTTTAAAGACCTCTTTGATAGGAATGTTTGATAGTGATGTCAACAAGCTAAATGTCAGTC
  AGCAGCCACTGCTGGATGTTGAAGGTAACTTAATAAAAATGCATGCAGCGGATTTGGAAAAGCCCATGGT
  AGAAAAGCAGGATCAATCCCCTTCATTAAGGACTGGAGAAGAAAAAAAAGACGTGTCCATTTCCAGACTG
  CGAGAGGCCTTTTCTCTTCGTCACACAACAGAGAACAAGCCTCACAGCCCAAAGACTCCAGAACCAAGAA
  GGAGCCCTCTAGGACAGAAAAGGGGTATGCTGTCTTCTAGCACTTCAGGTGCCATCTCTGACAAAGGCGT
  CCTGAGACCTCAGAAAGAGGCAGTGAGTTCCAGTCACGGACCCAGTGACCCTACGGACAGAGCGGAGGTG
  GAGAAGGACTCGGGGCACGGCAGCACTTCCGTGGATTCTGAGGGGTTCAGCATCCCAGACACGGGCAGTC
  ACTGCAGCAGCGAGTATGCGGCCAGCTCCCCAGGGGACAGGGGCTCGCAGGAACATGTGGACTCTCAGGA
  GAAAGCGCCTGAAACTGACGACTCTTTTTCAGATGTGGACTGCCATTCAAACCAGGAAGATACCGGATGT
  AAATTTCGAGTTTTGCCTCAGCCAACTAATCTCGCAACCCCAAACACAAAGCGTTTTAAAAAAGAAGAAA
  TTCTTTCCAGTTCTGACATTTGTCAAAAGTTAGTAAATACTCAGGACATGTCAGCCTCTCAGGTTGATGT
  AGCTGTGAAAATTAATAAGAAAGTTGTGCCCCTGGACTTTTCTATGAGTTCTTTAGCTAAACGAATAAAG
  CAGTTACATCATGAAGCACAGCAAAGTGAAGGGGAACAGAATTACAGGAAGTTTAGGGCAAAGATTTGTC
  CTGGAGAAAATCAAGCAGCCGAAGATGAACTAAGAAAAGAGATAAGTAAAACGATGTTTGCAGAAATGGA
  AATCATTGGTCAGTTTAACCTGGGATTTATAATAACCAAACTGAATGAGGATATCTTCATAGTGGACCAG
  CATGCCACGGACGAGAAGTATAACTTCGAGATGCTGCAGCAGCACACCGTGCTCCAGGGGCAGAGGCTCA
  TAGCACCTCAGACTCTCAACTTAACTGCTGTTAATGAAGCTGTTCTGATAGAAAATCTGGAAATATTTAG
  AAAGAATGGCTTTGATTTTGTTATCGATGAAAATGCTCCAGTCACTGAAAGGGCTAAACTGATTTCCTTG
  CCAACTAGTAAAAACTGGACCTTCGGACCCCAGGACGTCGATGAACTGATCTTCATGCTGAGCGACAGCC
  CTGGGGTCATGTGCCGGCCTTCCCGAGTCAAGCAGATGTTTGCCTCCAGAGCCTGCCGGAAGTCGGTGAT
  GATTGGGACTGCTCTTAACACAAGCGAGATGAAGAAACTGATCACCCACATGGGGGAGATGGACCACCCC
  TGGAACTGTCCCCATGGAAGGCCAACCATGAGACACATCGCCAACCTGGGTGTCATTTCTCAGAACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000535 unedited
  AGAATTTGTATACGACTCATATAGGCGGCCGCGNATTCGGCACGAGGCTGGAGGGAACTT
  TCCCAGTCCCCGAGGCGGATCGGGTGTTGCATCCATGGAGCGAGCTGAGAGCTCGAGTAC
  AGAACCTGCTAAGGCCATCAAACCTATTGATCGGAAGTCAGTCCATCAGATTTGCTCTGG
  GCAGGTGGTACTGAGTCTAAGCACTGCGGTAAAGGAGTTAGTAGAAAACAGTCTGGATGC
  TGGTGCCACTAATATTGATCTAAAGCTTAAGGACTATGGAGTGGATCTTATTGAAGTTTC
  AGACAATGGATGTGGGGTAGAAGAAGAAAACTTCGAAGGCTTAACTCTGAAACATCACAC
  ATCTAAGATTCAAGAGTTTGCTGACCTAACTCAGGTTGAAACTTTTGGCTTTCGGGGGGA
  AGCTCTGAGCTCACTTTGTGCACTGAGCGATGTCACCATTTCTACCTGCCACGCATCGGC
  GAAGGTTGGAACTCGACTGATGTTTGATCACAATGGGAAAATTATCCAGAAAACCCCCTA
  CCCCCGCCCCAGAGGGACCACAGTCAGCGTGCAGCAGTTATTTTCCACACTACCTGTGCG
  CCATAAGGAATTTCAAAGGAATATTAAGAAGGAGTATGCCAAAATGGTCCAGGTCTTACA
  TGCATACTGTATCATTTCAGCAGGCATCCGTGTAAGTTGCACCAATCAGCTTGGACAAGG
  AAAACGACAGCCTGTGGTATGCACAGGTGGAAGCCCCAGCATAAAGGAAAATATCGGCTC
  TGTGTTTGGGCAGAAGCAGTTGCAAAGCCTCATTCCTTTTGTTCAGCTGCCCCCTAGTGA
  CTNCGTGTGTGAAGAGTACGGTTTGAGCTGTTCNGATGCTCTGCATAATCTTTTTACATC
  TCAGGNTTCATTTCACATGCACGCTGGANNTGGGGGNAGTTCACAGACAGACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000535 unedited
  GCCCGAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGTTTTGGTTTTTGAAACACAGTC
  TTGCTCTATCTCACCCAGGCTGGAGCGCAGTGGCGCAATCTCGGTTCACTGCAACCTCCG
  TCTCCCGGGCCCAAGCGATTCTCCCGCCTCAGCCTCCTGGGTAGCGGGGATCACAGGCAT
  GCGCCAGCACACCTGGCTAATTTTGTATTTTTAGTAAAGGCAGGGTTTCTCCATGTTGGT
  CAAGCTGGTCTCGAACTTCTGATCTCAGGTGATCCGCCGGCCTCGGCCTCCCAAAGTGCT
  GCGATCACAGGCATGAGCCACCATGCCCAGCCCAAGTGTTCTTATTTTTATAAAATGTGT
  TCTTGCCTGGACACACACACACGAGCGCATGCAAACATAGAGAAAAAAAATTTGCAAGCA
  ATGCTCCATCTGGTTTGAAAAGGGTCTCAAGATCACTTTTAAATGGGTGTGATGTGTATT
  TTTTTTAAGTAGCAGGTTCATTTCAAAACAAAAAAGGTTAGTGAAGACTCTGTCTTTCAA
  AACATAAAAATCTGCGATAAAACCAATTATTCCATACAGTGACTACGGTCAGTTCTGAGA
  AATGACACCCAAGTTGGCGATGTGTCTCATGTTTGGCCTTCCATGGGGACAGTTCCAGAG
  GTGGTCCATTTCCCCCATGTGGGAGATCAGTTTCTTCATTTCGCTTGTGGTTAAAAGCAG
  TCCCAATCATTACCGACTTCCGGCAGGGCTCTGTGAGGCAAACATCTGCTTTGACTCTCG
  GAAAGGCCGGAACAAGAACCCCAAGGCCTGGCGCCCAACCTTGATTATCATTTAATTCAA
  CGCACTGGGTGCCCGAAGTGCCAATTTCTACATTATTGGCAAGAAAAACATTTATACCCT
  ATACAAGACTAGAGCACTTCTTTCCGCTAACATAAATAAAGCCACTCCTTTCTTAAATAT
  TCCCACTATTTTTTTAACAACCCTCCAANATAACCAGGTAGTCGAGAGACTCGAGTGCCT
  ACAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000535
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000535.3, NP_000526.1
• RefSeq Size: 2851 bp
• RefSeq ORF: 2589 bp
• Locus ID: 5395
• Cytogenetics: 7p22.1
• Domains: DNA_mis_repair, HATPase_c
• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair
• Gene Summary: 'The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome. [provided by RefSeq, Apr 2016]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.