CYP1A1 (NM_000499) Human Untagged Clone

CAT#: SC119858

CYP1A1 (untagged)-Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP1A1
• Synonyms: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000499, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTTTCCCAATCTCCATGTCGGCCACGGAGTTTCTTCTGGCCTCTGTCATCTTCTGTCTGGTATTCT
  GGGTAATCAGGGCCTCAAGACCTCAGGTCCCCAAAGGCCTGAAGAATCCACCAGGGCCATGGGGCTGGCC
  TCTGATTGGGCACATGCTGACCCTGGGAAAGAACCCGCACCTGGCACTGTCAAGGATGAGCCAGCAGTAT
  GGGGACGTGCTGCAGATCCGAATTGGCTCCACACCCGTGGTGGTGCTGAGCGGCCTGGACACCATCCGGC
  AGGCCCTGGTGCGGCAGGGCGATGATTTCAAGGGCCGGCCCGACCTCTACACCTTCACCCTCATCAGTAA
  TGGTCAGAGCATGTCCTTCAGCCCAGACTCTGGACCAGTGTGGGCTGCCCGCCGGCGCCTGGCCCAGAAT
  GGCCTGAAAAGTTTCTCCATTGCCTCTGACCCAGCCTCCTCAACCTCCTGCTACCTGGAAGAGCATGTGA
  GCAAGGAGGCTGAGGTCCTGATAAGCACGTTGCAGGAGCTGATGGCAGGGCCTGGGCACTTTAACCCCTA
  CAGGTATGTGGTGGTATCAGTGACCAATGTCATCTGTGCCATTTGCTTTGGCCGGCGCTATGACCACAAC
  CACCAAGAACTGCTTAGCCTAGTCAACCTGAATAATAATTTCGGGGAGGTGGTTGGCTCTGGAAACCCAG
  CTGACTTCATCCCTATTCTTCGCTACCTACCCAACCCTTCCCTGAATGCCTTCAAGGACCTGAATGAGAA
  GTTCTACAGCTTCATGCAGAAGATGGTCAAGGAGCACTACAAAACCTTTGAGAAGGGCCACATCCGGGAC
  ATCACAGACAGCCTGATTGAGCACTGTCAGGAGAAGCAGCTGGATGAGAACGCCAATGTCCAGCTGTCAG
  ATGAGAAGATCATTAACATCGTCTTGGACCTCTTTGGAGCTGGGTTTGACACAGTCACAACTGCTATCTC
  CTGGAGCCTCATGTATTTGGTGATGAACCCCAGGGTACAGAGAAAGATCCAAGAGGAGCTAGACACAGTG
  ATTGGCAGGTCACGGCGGCCCCGGCTCTCTGACAGATCCCATCTGCCCTATATGGAGGCCTTCATCCTGG
  AGACCTTCCGACACTCTTCCTTCGTCCCCTTCACCATCCCCCACAGCACAACAAGAGACACAAGTTTGAA
  AGGCTTTTACATCCCCAAGGGGCGTTGTGTCTTTGTAAACCAGTGGCAGATCAACCATGACCAGAAGCTA
  TGGGTCAACCCATCTGAGTTCCTACCTGAACGGTTTCTCACCCCTGATGGTGCTATCGACAAGGTGTTAA
  GTGAGAAGGTGATTATCTTTGGCATGGGCAAGCGGAAGTGTATCGGTGAGACCATTGCCCGCTGGGAGGT
  CTTTCTCTTCCTGGCTATCCTGCTGCAACGGGTGGAATTCAGCGTGCCACTGGGCGTGAAGGTGGACATG
  ACCCCCATCTATGGGCTAACCATGAAGCATGCCTGCTGTGAGCACTTCCAAATGCAGCTGCGCTCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000499 unedited
  GCAACCCANTCGCACCAGGTTCCTTGGAACCTTCCCTGATCCTTGTGATCCCAGGCTCCA
  AAAGTCCACCCTTCCCAGCTCAGCTCAGTACCTCAGCAGCCACCTCCAAGATCCCTACAC
  TGATCATGCTTTTCCCAATCTCCATGTCGGCCACGGAGTTTCTTCTGGCCTCTGTCATTT
  NCTGNCTGGTATTCTGGGTAATCAGGGCCTCAAGACCTCATGTCCCCAAAGGCCTGAAGA
  ATCCACCAGGGCCATGGGGCTGGCCTCTGATTGGGCACATGCTGACCCTGGGAAAGAACC
  CGCACCTGGCACTGTCAAGGATGAGCCAGCAGTATGGGGACGTGCTGCAGATCCGAATTG
  GCTCCACACCCGTGGTGGTGCTGAGCGGCCTGGACACCATCCGGCAGGCCCTGGTGCGGC
  AGGGCGATGATTTCAAGGGCCGGCCCGACCTCTACACCTTCACCCTCATCAGTAATGGTC
  AGAGCATGTCCTTCAGCCCAGACTCTGGACCAGTGTGGGCTGCCCGCCGGCGCCTGGCCC
  AGAATGGCCTGAAAAGTTTCTCCATTGCCTCTGACCCAGCCTCCTCAACCTCCTGCTACC
  TGGAAGAGCATGTGAGCAAGGAGGCTGAGGTCCTGATAAGCACGTTGCANGAGCTGATGG
  CAGGGCCTGGGCACTTTAACCCCTACAGGTATGTGGTGGGATCAGTGACCAATGTCATCT
  GTGCCATTTGCTTTGGCCGGCGCTATGACCACAACCACCAAAAACTGCTTAGCCTAGTCA
  ACCTGAATGCCCTTCAGGACCTGGATGAGGAGGTCTACAGCTTCATGCAGAAGATGGTCA
  AAGAGCACTACAAAACTTTGAGAAAGCCACATCCGGGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000499 unedited
  CGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCT
  ATGACTTCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGTTTTTTTCTGAACAATATCT
  TTATTTAGCTCAGGTAGTTGTTCTTACACATTTTTGGATACCTGGGTTAGAATTTTCCCT
  ATTACATTAAATCAATGGTTCTTAAGCTCTTATGCAAGCATGCAAGCTCAATGCAGGCTA
  GAATAGAAGGATTGTTGTGTACAAACATTAAAAATAGACATTTTATTACAAGAGTGTAGA
  GAAGGGAGACCAATAGAAGGTAATTGAAATACCCCCCCCTCACTCCAGCCCTAGTCCTGG
  TGCCTGGATATGTGCACTCCCTGTGCGCTCTGATCCCCGCAGACACAAGTCCCCAGCCCC
  TCCAGGACAGCAATAAGGGTCTTACAAGGCCAGAAGGCAGCCCTGTTTGTTCCTGCCTGC
  AGGAAGGGCAGAGGAATGTGATGTTCCCAGGAACTGTGTCCTAGACCCATAGGGTCAGAT
  TGCTCAGCCTAGTTCAAGCAGTGAGACTACCTCTGTGCCAGTATCCTGGGCTGTCTCTTC
  CNTTCACTCTTGGCAGCTCCCAATTGTCAAAGATTGGACAGGGGTCTGGGTTGGGCTAGT
  CTAACTTGCTGAAGCCAGTCAGCACCCTCACAGAGCCAGCTAGGTACTGGGCCCAGGGGC
  TTCCAGAGAGTTCTTCAGAGCTTCTCAGAGGCCTAAGGACCTCCTAACCCTAGCAGGGCC
  TCCTGCTCAAGCACAACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000499
• Insert Size: 2310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000499.2, NP_000490.1
• RefSeq Size: 2602 bp
• RefSeq ORF: 1539 bp
• Locus ID: 1543
• Cytogenetics: 15q24.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tryptophan metabolism
• Gene Summary: 'This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 3 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.