CYP1A1 (NM_000499) Human Untagged Clone

CAT#: SC322548

CYP1A1 (untagged)-Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP1A1
• Synonyms: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322548 GTGATCCCAGGCTCCAAGAGTCCACCCTTCCCAGCTCAGCTCAGTACCTCAGCAGCCACC
  TCCAAGATCCCTACACTGATCATGCTTTTCCCAATCTCCATGTCGGCCACGGAGTTTCTT
  CTGGCCTCTGTCATCTTCTGTCTGGTATTCTGGGTAATCAGGGCCTCAAGACCTCAGGTC
  CCCAAAGGCCTGAAGAATCCACCAGGGCCATGGGGCTGGCCTCTGATTGGGCACATGCTG
  ACCCTGGGAAAGAACCCGCACCTGGCACTGTCAAGGATGAGCCAGCAGTATGGGGACGTG
  CTGCAGATCCGAATTGGCTCCACACCCGTGGTGGTGCTGAGCGGCCTGGACACCATCCGG
  CAGGCCCTGGTGCGGCAGGGCGATGATTTCAAGGGCCGGCCCGACCTCTACACCTTCACC
  CTCATCAGTAATGGTCAGAGCATGTCCTTCAGCCCAGACTCTGGACCAGTGTGGGCTGCC
  CGCCGGCGCCTGGCCCAGAATGGCCTGAAAAGTTTCTCCATTGCCTCTGACCCAGCCTCC
  TCAACCTCCTGCTACCTGGAAGAGCATGTGAGCAAGGAGGCTGAGGTCCTGATAAGCACG
  TTGCAGGAGCTGATGGCAGGGCCTGGGCACTTTAACCCCTACAGGTATGTGGTGGTATCA
  GTGACCAATGTCATCTGTGCCATTTGCTTTGGCCGGCGCTATGACCACAACCACCAAGAA
  CTGCTTAGCCTAGTCAACCTGAATAATAATTTCGGGGAGGTGGTTGGCTCTGGAAACCCA
  GCTGACTTCATCCCTATTCTTCGCTACCTACCCAACCCTTCCCTGAATGCCTTCAAGGAC
  CTGAATGAGAAGTTCTACAGCTTCATGCAGAAGATGGTCAAGGAGCACTACAAAACCTTT
  GAGAAGGGCCACATCCGGGACATCACAGACAGCCTGATTGAGCACTGTCAGGAGAAGCAG
  CTGGATGAGAACGCCAATGTCCAGCTGTCAGATGAGAAGATCATTAACATCGTCTTGGAC
  CTCTTTGGAGCTGGGTTTGACACAGTCACAACTGCTATCTCCTGGAGCCTCATGTATTTG
  GTGATGAACCCCAGGGTACAGAGAAAGATCCAAGAGGAGCTAGACACAGTGATTGGCAGG
  TCACGGCGGCCCCGGCTCTCTGACAGATCCCATCTGCCCTATATGGAGGCCTTCATCCTG
  GAGACCTTCCGACACTCTTCCTTCGTCCCCTTCACCATCCCCCACAGCACAACAAGAGAC
  ACAAGTTTGAAAGGCTTTTACATCCCCAAGGGGCGTTGTGTCTTTGTAAACCAGTGGCAG
  ATCAACCATGACCAGAAGCTATGGGTCAACCCATCTGAGTTCCTACCTGAACGGTTTCTC
  ACCCCTGATGGTGCTATCGACAAGGTGTTAAGTGAGAAGGTGATTATCTTTGGCATGGGC
  AAGCGGAAGTGTATCGGTGAGACCATTGCCCGCTGGGAGGTCTTTCTCTTCCTGGCTATC
  CTGCTGCAACGGGTGGAATTCAGCGTGCCACTGGGCGTGAAGGTGGACATGACCCCCATC
  TATGGGCTAACCATGAAGCATGCCTGCTGTGAGCACTTCCAAATGCAGCTGCGCTCTTAG
  GTGCTTGAGAGCCCTGAGGCCTAGACTCTGTCTACCTGGTCTGGTTGGGCAGCCAGACCA
  GCAGGCTGGCCTATGTGGTCTAAGGTTCAGCCTGAAACTCATAGACACTGATCTGGCTGC
  AGTTTTGCTATCTGGGCTGTGGGCAAGCCTAAGGGATCCTGCCTGCCCCTACCCTGGACT
  TGCCTCTGCACACCCTCCAGAGACAACAGGTAAAACAGGGCCACATAGATGCTGATGGAG
  CCTTCCCAAGTTGTGCTTGAGCCAGGAGGCCTGCTAGGGTTAGGAGGTCCTTAGGCCTCT
  GAGAAGCTCTGAAGAACTCTCTGGAAGCCCCTGGGCCCAGTACCTAGCTGGCTCTGTGAG
  GGTGCTGACTGGCTTCAGCAAGTTAGAACTAGCCAAACCAGGACCCTGTCCAATCTTTGA
  CAATTGGGAGCTGCCAAGAGTGAAGGGAAGAGACAGCCCAGGATACTGGCACAGAGGTAG
  TCTCACTGCTTGAACTAGGCTGAGCAATCTGACCCTATGGGTCTAGGACACAGTTCCTGG
  GAACATCACATTCCTCTGCCCTTCCTGCAGGCAGGAACAAACAGGGCTGCCTTCTGGCCT
  TGTAAGACCCTTATTGCTGTCCTGGAGGGGCTGGGGACTTGTGTCTGCGGGGATCAGAGC
  GCACAGGGAGTGCACATATCCAGGCACCAGGACTAGGGCTGGAGTGAGGGGGGGGTATTT
  CAATTACCTTCTATTGGTCTCCCTTCTCTACACTCTTGTAATAAAATGTCTATTTTTAAT
  GTTTGTACACAACAATCCTTCTATTCTAGCCTGCATTGAGCTTGCATGCTTGCATAAGAG
  CTTAAGAACCATTGATTTAATGTAATAGGGAAAATTCTAACCCAGGTATCCAAAAATGTG
  TAAGAACAACTACCTGAGCTAAATAAAGATATTGTTCAGAAATCCTAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000499
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000499.2, NP_000490.1
• RefSeq Size: 2602 bp
• RefSeq ORF: 1539 bp
• Locus ID: 1543
• Cytogenetics: 15q24.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tryptophan metabolism
• Gene Summary: 'This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 3 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.