KEL (NM_000420) Human Untagged Clone

CAT#: SC119878

KEL (untagged)-Human Kell blood group, metallo-endopeptidase (KEL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KEL
• Synonyms: CD238; ECE3; Kell
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000420, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGGTGGGGACCAAAGTGAGGAAGAGCCGAGGGAACGCAGCCAGGCAGGTGGAATGGGAACTCTCT
  GGAGCCAAGAGAGCACTCCAGAAGAGAGGCTGCCCGTGGAAGGGAGCAGGCCATGGGCAGTGGCCAGGCG
  GGTGCTGACAGCTATCCTGATTTTGGGCCTGCTCCTTTGTTTTTCTGTGCTTTTGTTCTACAACTTCCAG
  AACTGTGGCCCTCGCCCCTGTGAGACATCTGTGTGTTTGGATCTCCGGGATCATTACCTGGCCTCTGGGA
  ACACAAGTGTGGCCCCCTGCACCGACTTCTTCAGCTTTGCCTGTGGAAGGGCCAAAGAGACCAATAATTC
  TTTTCAGGAGCTTGCCACAAAGAACAAAAACCGACTTCGGAGAATACTGGAGGTCCAGAATTCCTGGCAC
  CCAGGCTCTGGGGAGGAGAAAGCCTTCCAGTTCTACAACTCCTGCATGGATACACTTGCCATTGAAGCTG
  CAGGGACTGGTCCCCTCAGACAAGTTATTGAGGAGCTTGGAGGCTGGCGCATCTCTGGTAAATGGACTTC
  CTTAAACTTTAACCGAACGCTGAGACTTCTGATGAGTCAGTATGGCCATTTCCCTTTCTTCAGAGCCTAC
  CTAGGACCTCATCCTGCCTCTCCACACACACCAGTCATCCAGATAGACCAGCCAGAGTTTGATGTTCCCC
  TCAAGCAAGATCAAGAACAGAAGATCTATGCCCAGATCTTTCGGGAATACCTGACTTACCTGAATCAGCT
  GGGAACCTTGCTGGGAGGAGACCCAAGCAAGGTGCAAGAACACTCTTCCTTGTCAATCTCCATCACTTCA
  CGGCTGTTCCAGTTTCTGAGGCCCCTGGAGCAGCGGCGGGCACAGGGCAAGCTCTTCCAGATGGTCACTA
  TCGACCAGCTCAAGGAAATGGCCCCCGCCATCGACTGGTTGTCCTGCTTGCAAGCGACATTCACACCGAT
  GTCCCTGAGCCCTTCTCAGTCCCTCGTGGTCCATGACGTGGAATATTTGAAAAACATGTCACAACTGGTG
  GAGGAGATGCTGCTAAAGCAGAGGGACTTTCTGCAGAGCCACATGATCTTAGGGCTGGTGGTGACCCTTT
  CTCCAGCCCTGGACAGTCAATTCCAGGAGGCACGCAGAAAGCTCAGCCAGAAACTGCGGGAACTGACAGA
  GCAACCACCCATGCCTGCCCGCCCACGATGGATGAAGTGCGTGGAGGAGACAGGCACGTTCTTCGAGCCC
  ACGCTGGCGGCTTTGTTTGTTCGTGAGGCCTTTGGCCCGAGCACCCGAAGTGCTGCCATGAAATTATTCA
  CTGCGATCCGGGATGCCCTCATCACTCGCCTCAGAAACCTTCCCTGGATGAATGAGGAGACCCAGAACAT
  GGCCCAGGACAAGGTTGCTCAACTGCAGGTGGAGATGGGGGCTTCAGAATGGGCCCTGAAGCCAGAGCTG
  GCCCGACAAGAATACAACGATATACAGCTTGGATCGAGCTTCCTGCAGTCTGTCCTGAGCTGTGTCCGGT
  CCCTCCGAGCTAGAATTGTCCAGAGCTTCTTGCAGCCTCACCCCCAACACAGGTGGAAGGTGTCCCCTTG
  GGACGTCAATGCTTACTATTCGGTATCTGACCATGTGGTAGTCTTTCCAGCTGGACTCCTCCAACCCCCA
  TTCTTCCACCCTGGCTATCCCAGAGCCGTGAACTTTGGCGCTGCTGGCAGCATCATGGCCCACGAGCTGT
  TGCACATCTTCTACCAGCTCTTACTGCCTGGGGGCTGCCTCGCCTGTGACAACCATGCCCTCCAGGAAGC
  TCACCTGTGCCTGAAGCGCCATTATGCTGCCTTTCCATTACCTAGCAGAACCTCCTTCAATGACTCCCTC
  ACATTCTTAGAGAATGCTGCAGACGTTGGGGGGCTAGCCATCGCGCTGCAGGCATACAGCAAGAGGCTGT
  TACGGCACCATGGGGAGACTGTCCTGCCCAGCCTGGACCTCAGCCCCCAGCAGATCTTCTTTCGAAGCTA
  TGCCCAGGTGATGTGTAGGAAGCCCAGCCCCCAGGACTCTCACGACACTCACAGCCCTCCACACCTCCGA
  GTCCACGGGCCCCTCAGCAGCACCCCAGCCTTTGCCAGGTATTTCCGCTGTGCACGTGGTGCTCTCTTGA
  ACCCCTCCAGCCGCTGCCAGCTCTGGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000420
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000420.2, NP_000411.1
• RefSeq Size: 2562 bp
• RefSeq ORF: 2199 bp
• Locus ID: 3792
• Cytogenetics: 7q34
• Domains: Peptidase_M13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008]'