NCF2 (NM_000433) Human Untagged Clone

CAT#: SC119885

NCF2 (untagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCF2
• Synonyms: NCF-2; NOXA2; P67-PHOX; P67PHOX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119885 sequence for NM_000433 edited (data generated by NextGen Sequencing)
  ATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAG
  GACTGGAAGGGAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGC
  TTCAACATTGGCTGCATGTACACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTT
  ACCAGAAGCATTAACCGAGACAAGCACTTGGCAGTGGCTTACTTCCAACGAGGGATGCTC
  TACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACCTTAAAGAAGCCTTGATTCAG
  CTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTCAAGCTGTTTGCC
  TGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAATGGAAAAAAGCT
  GAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCAAAATCGAC
  AAGGCGATGGAGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTGATCCCTGTGGGC
  AAGCTGTTTCGACCAAATGAGAGACAAGTGGCTCAGCTGGCCAAGAAGGATTACCTAGGC
  AAGGCGACGGTCGTGGCATCTGTGGTGGATCAAGACAGTTTCTCTGGGTTTGCCCCTCTG
  CAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCCCAGAGATCTTCAGGGCTCTG
  GAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAAGAAGAGCTCCAG
  GTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAACTGGGCCACGGTC
  ATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCAGTTGAGCTGCGG
  ATCCACCCTCAGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGACATCCCAGCTCCT
  CCTAGTTCCAAAGCCCCTGGAAGACCCCAGCTGTCACCAGGCCAGAAACAAAAAGAAGAG
  CCTAAGGAAGTGAAGCTCAGTGTTCCCATGCCCTACACACTCAAGGTGCACTACAAGTAC
  ACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCCAGGTCCGGGACATGGTGTCT
  AAGAAACTGGAGCTCCGGCTGGAACACACTAAGCTGAGCTATCGGCCTCGGGACAGCAAT
  GAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAGGTGAAAAACTAC
  TGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCAGATGAACCCAAG
  GAAAGTGAAAAAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTTAAGAAAGGCAGC
  CAAGTGGAGGCACTCTTCAGTTATGAGGCTACCCAACCAGAGGACCTGGAGTTTCAGGAA
  GGGGATATAATCCTGGTGTTATCAAAGGTGAATGAAGAATGGCTGGAAGGGGAGTGCAAA
  GGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACTGCGCAACTACAGATTTGGAA
  AGCACTCGGAGAGAAGTCTAG
  
  Clone variation with respect to NM_000433.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000433 unedited
  GCCCTTGNCATTTGTACACGACTCATATAGGCGGCCGCGAATTCGGCACCAGTATAAAGT
  GAACACCTTGGGGGCCACTAAGGCAGCCCTGCTAGGGGAGACGCTCCAACCTGTCTTCTC
  TCTGTCTCCTGGCAGCTCTCTTGGCCTCCTAGTTTCTACCTAATCATGTCCCTGGTGGAG
  GCCATCAGCCTCTGGAATGAAGGGGTGCTGGCAGCGGACAAGAAGGACTGGAAGGGAGCC
  CTGGATGCCTTCAGTGCCGTCCAGGACCCCCACTCCCGGATTTGCTTCAACATTGGCTGC
  ATGTACACTATCCTGAAGAACATGACTGAAGCAGAGAAGGCCTTTACCAGAAGCATTAAC
  CGAGACAAGCACTTGGCAGTGGCTTACTTCCAACGAGGGATGCTCTACTACCAGACAGAG
  AAATATGATTTGGCTATCAAAGACCTTAAAGAAGCCTTGATTCAGCTTCGAGGGAACCAG
  CTGATAGACTATAAGATCCTGGGGCTCCAGTTCAAGCTGTTTGCCTGTGAGGTGTTATAT
  AACATTGCTTTCATGTATGCCAAGAAGGATGAATGGAAAAATAGCTGAAGATCAGTTAGC
  ATTGGCCACGAGCATGAAGTCTGAGCCCAGACATTCCATAATCGACAGGCGATGGAGTGT
  GTCTGGAATCANAAGCATATGATCCAGTGGTGATCCCTGNTGGGCAGCTGTTTCGACCAA
  TGAGAGACAGTGGCTCAGCTGGCCNAGAAGGATTACCCTAGCAGGCGACGGTCGTGCATC
  TGGGNGGATCAAGAAGTTTCCCTGGTTTGGCCCTCTGAACCCACAGCAGCTGACCTCCCC
  CCGGACCGAAACCCCGAAATCTCAGGCCTCGAAAGGGAGCCTAACGGGGCATTTGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000433 unedited
  TTTTTTTTTTTTAGGTTTGAAAAAAAAAAACAAACAAGTTTATTTGTATATGCCTTATGA
  GTAACCTATAAGGTTACTTCAAGCCTTAAGAGCCAATTACAGTAATGGTTCAGAAACAGG
  AATATTTGTACTTACTCCTTGGGCCTGGACTTGGGTGTCTTGTTTTTGTAAGATGATGAT
  AGGTTTTTTTTTATTGTGGTATGACACAGAAAAGTGCACCAAATGTACAGCTTGATGAAT
  GTTTACAGTGTGAACACAGCTGGCTAGCTAGCACTCAGAGCAAGAAACAGGATCAGTACC
  TGGAAGACTCTCTCGTGCCCTTTCCAGACACTTCCATCCACTTTTCCTCTCCCCTAACTC
  CTCCATTCACCTGTCCCCATCTCCTCACCCACTGTGCCCCAGGAAATCATCCTGNGTACT
  CACATCATTGTCTAGTAGGTTAAATTTTAACAGGGAATAAATAGTTAACACTTCCAAACT
  GTAATGTCTCAGTACAGTATACAGCAGAAGGGTGCTAAATCTTACAAACAAGTAATAGGG
  CTTCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000433
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000433.2, NP_000424.2
• RefSeq Size: 2406 bp
• RefSeq ORF: 1581 bp
• Locus ID: 4688
• Cytogenetics: 1q25.3
• Domains: PB1, TPR, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: 'This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) and variant 2 encode the same protein (isoform 1).