NCF2 (NM_000433) Human Untagged Clone

CAT#: SC320681

NCF2 (untagged)-Human neutrophil cytosolic factor 2 (NCF2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCF2
• Synonyms: NCF-2; NOXA2; P67-PHOX; P67PHOX
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000433.2 TCTCTCTCTGCTTCTTTCCTTTTCTCTCTCATGGTAGGGTTATGAGTCAGTTGCCAAAAG
  GTGGGGACATTTCCTGATGCATTTGCAACACTGAGAAGTTATCTTAAGGGAGGCTGGGCC
  CCATTCTACTCATCTGGCCCAGAAAGTGAACACCTTGGGGGCCACTAAGGCAGCCCTGCT
  AGGGGAGACGCTCCAACCTGTCTTCTCTCTGTCTCCTGGCAGCTCTCTTGGCCTCCTAGT
  TTCTACCTAATCATGTCCCTGGTGGAGGCCATCAGCCTCTGGAATGAAGGGGTGCTGGCA
  GCGGACAAGAAGGACTGGAAGGGAGCCCTGGATGCCTTCAGTGCCGTCCAGGACCCCCAC
  TCCCGGATTTGCTTCAACATTGGCTGCATGTACACTATCCTGAAGAACATGACTGAAGCA
  GAGAAGGCCTTTACCAGAAGCATTAACCGAGACAAGCACTTGGCAGTGGCTTACTTCCAA
  CGAGGGATGCTCTACTACCAGACAGAGAAATATGATTTGGCTATCAAAGACCTTAAAGAA
  GCCTTGATTCAGCTTCGAGGGAACCAGCTGATAGACTATAAGATCCTGGGGCTCCAGTTC
  AAGCTGTTTGCCTGTGAGGTGTTATATAACATTGCTTTCATGTATGCCAAGAAGGAGGAA
  TGGAAAAAAGCTGAAGAACAGTTAGCATTGGCCACGAGCATGAAGTCTGAGCCCAGACAT
  TCCAAAATCGACAAGGCGATGGAGTGTGTCTGGAAGCAGAAGCTATATGAGCCAGTGGTG
  ATCCCTGTGGGCAGGCTGTTTCGACCAAATGAGAGACAAGTGGCTCAGCTGGCCAAGAAG
  GATTACCTAGGCAAGGCAACGGTCGTGGCATCTGTGGTGGATCAAGACAGTTTCTCTGGG
  TTTGCCCCTCTGCAACCACAGGCAGCTGAGCCTCCACCCAGACCGAAAACCCCAGAGATC
  TTCAGGGCTCTGGAAGGGGAGGCTCACCGTGTGCTATTTGGGTTTGTGCCTGAGACAAAA
  GAAGAGCTCCAGGTCATGCCAGGGAACATTGTCTTTGTCTTGAAGAAGGGCAATGATAAC
  TGGGCCACGGTCATGTTCAACGGGCAGAAGGGGCTTGTTCCCTGCAACTACCTTGAACCA
  GTTGAGCTGCGGATCCACCCTCAGCAGCAGCCCCAGGAGGAAAGCTCTCCGCAGTCCGAC
  ATCCCAGCTCCTCCTAGTTCCAAAGCCCCTGGAAGACCCCAGCTGTCACCAGGCCAGAAA
  CAAAAAGAAGAGCCTAAGGAAGTGAAGCTCAGTGTTCCCATGCCCTACACACTCAAGGTG
  CACTACAAGTACACGGTAGTCATGAAGACTCAGCCCGGGCTCCCCTACAGCCAGGTCCGG
  GACATGGTGTCTAAGAAACTGGAGCTCCGGCTGGAACAAACTAAGCTGAGCTATCGGCCT
  CGGGACAGCAATGAGCTGGTGCCCCTTTCAGAAGACAGCATGAAGGATGCCTGGGGCCAG
  GTGAAAAACTACTGCCTGACTCTGTGGTGTGAGAACACAGTGGGTGACCAAGGCTTTCCA
  GATGAACCCAAGGAAAGTGAAAAAGCTGATGCTAATAACCAGACAACAGAACCTCAGCTT
  AAGAAAGGCAGCCAAGTGGAGGCACTCTTCAGTTATGAGGCTACCCAACCAGAGGACCTG
  GAGTTTCAGGAAGGGGATATAATCCTGGTGTTATCAAAGGTGAATGAAGAATGGCTGGAA
  GGGGAGTGCAAAGGGAAGGTGGGCATTTTCCCCAAAGTTTTTGTTGAAGACTGCGCAACT
  ACAGATTTGGAAAGCACTCGGAGAGAAGTCTAGGATGTTTCACAAACTACAAAGCTGAAG
  AAAATGAAGCCCTATTACTTGTTTGTAAGATTTAGCACCCTTCTGCTGTATACTGTACTG
  AGACATTACAGTTTGGAAGTGTTAACTATTTATTCCCTGTTAAAATTTAACCTACTAGAC
  AATGATGTGAGTACCCAGGATGATTTCCTGGGGCACAGTGGGTGAGGAGATGGGGACAGG
  TGAATGGAGGAGTTAGGGGAGAGGAAAAGTGGATGGAAGTGTCTGGAAAGGGCACGAGAG
  AGTCTTCCAGGTACTGATCCTGTTTCTTGCTCTGAGTGCTAGCTAGCCAGCTGTGTTCAC
  ACTGTAAACATTCATCAAGCTGTACATTTGGTGCACTTTTCTGTGTCATACCACAAAAAA
  AAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000433
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000433.2, NP_000424.2
• RefSeq Size: 2406 bp
• RefSeq ORF: 1581 bp
• Locus ID: 4688
• Cytogenetics: 1q25.3
• Domains: PB1, TPR, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: 'This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) and variant 2 encode the same protein (isoform 1).