GPD2 (NM_000408) Human Untagged Clone

CAT#: SC119913

GPD2 (untagged)-Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPD2
• Synonyms: GDH2; GPDM; mGDH; mGPDH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000408 edited
  GCGGCCGCGAATTCGGCACGAGGCTCGCTGGGAGCACCCGGGCCGAGGCTCTGATTCTGG
  GGGGAGGCCGACTCCACCCTGGCTGGAGGAACTGGGTGCTCCTGCCCGCTGGCCCCTCGC
  GCGTGAGGATCTATCTCAGGCTAAGAAATGGCATTTCAAAAGGCAGTGAAAGGGACGATT
  CTTGTTGGAGGAGGTGCTCTTGCAACTGTTTTAGGACTTTCTCAGTTTGCTCATTACAGA
  AGGAAACAAATGAACCTGGCCTATGTTAAAGCAGCAGACTGCATTTCAGAACCAGTTAAC
  AGGGAGCCTCCTTCCAGAGAAGCTCAGCTACTGACTTTGCAAAACACATCTGAATTTGAT
  ATCCTTGTTATTGGAGGAGGAGCAACAGGAAGTGGCTGTGCGCTAGATGCTGTCACCAGA
  GGACTAAAAACAGCCCTTGTAGAAAGAGATGATTTCTCATCAGGGACCAGCAGCAGAAGC
  ACTAAATTGATCCATGGTGGTGTGAGATATCTGCAGAAGGCCATCATGAAGTTGGATATT
  GAGCAGTATAGGATGGTAAAAGAAGCCCTTCATGAGCGTGCCAACCTGCTAGAAATTGCT
  CCCCATTTATCAGCTCCATTGCCTATAATGCTTCCAGTTTACAAGTGGTGGCAGTTACCT
  TACTACTGGGTAGGAATCAAGCTGTATGATTTGGTTGCAGGAAGCAATTGCCTAAAAAGC
  AGTTATGTCCTCAGCAAATCAAGAGCCCTTGAACATTTCCCAATGCTCCAGAAGGACAAA
  CTGGTAGGAGCAATTGTCTACTATGACGGACAACATAACGATGCACGGATGAACCTTGCC
  ATTGCTCTGACTGCTGCCAGGTATGGGGCTGCCACAGCCAATTACATGGAGGTAGTGAGC
  TTGCTCAAGAAGACAGACCCCCAGACAGGGAAAGTGCGTGTGAGCGGCGCACGGTGCAAG
  GATGTCCTCACAGGGCAGGAATTTGACGTGAGAGCCAAATGTGTTATCAATGCCACGGGA
  CCTTTCACGGACTCTGTGCGCAAAATGGATGATAAAGACGCAGCAGCTATCTGCCAGCCA
  AGTGCTGGTGTCCATATTGTGATGCCTGGTTATTACAGCCCAGAGAGCATGGGACTTCTT
  GACCCAGCGACCAGTGATGGGCGAGTTATTTTCTTCTTACCCTGGCAAAAGATGACGATC
  GCTGGCACTACTGATACTCCAACTGATGTTACACACCATCCAATTCCTTCAGAAGAAGAT
  ATCAACTTCATTTTGAATGAAGTGCGTAATTACCTGAGTTGTGATGTTGAAGTGAGAAGA
  GGGGATGTCCTGGCAGCATGGAGTGGAATCCGTCCTCTTGTTACAGACCCCAAATCTGCA
  GATACTCAGTCTATCTCCCGAAATCATGTTGTTGATATCAGTGAGAGTGGCCTTATTACT
  ATAGCAGGTGGAAAGTGGACAACTTATCGGTCTATGGCAGAAGATACCATAAATGCTGCT
  GTCAAAACTCATAATTTAAAAGCAGGACCAAGTAGAACAGTTGGGCTTTTCCTTCAAGGG
  GGTAAAGATTGGAGCCCCACACTCTACATTAGGCTTGTGCAGGATTATGGACTTGAAAGC
  GAGGTGGCACAGCATCTTGCCGCCACCTATGGTGATAAGGCCTTTGAGGTGGCCAAATGG
  CAAGTGTGACTGGCAAAAGGTGGCCTATTGTTGGAGTACGTCTTGTGTCAGAATTTCCAT
  ATATTGAAGCAGAGGTGAAATATGGGATTAAGGAGTATGCCTGCACTGCTGTGGATATGA
  TTTCACGTCGTACTCGCCTGGCCTTTCTAAATGTCCAGGCAGCAGAGGAAGCCCTACCCA
  GGATTGTTGAACTGATGGGCAGGGAACTGAATTGGGATGATTATAAGAAGCAGGAACAAC
  TTGAAACAGCCAGGAAGTTTCTATATTATGAAATGGGCTATAAATCTCGATCAGAACAGT
  TAACAGATCGCTCTGAAATTAGCCTACTGCCTTCAGACATTGACAGGTATAAGAAGAGAT
  TTCATAAGTTTGATGCAGACCAGAAAGGCTTTATTACCATTGTTGATGTTCAGCGTGTAT
  TAGAGAGTATCAATGTCCAAATGGATGAAAATACACTCCATGAAATTCTAAATGAAGTTG
  ATTTGAATAAAAATGGACAGGTTGAACTCAATGAATTTTTGCAGCTGATGAGTGCTATTC
  AAAAAGGAAGGGTATCTGGAAGCCGGCTTGCTATACTAATGAAAACTGCAGAAGAGAACC
  TCGACAGAAGAGTTCCAATTCCAGTGGACCGTAGTTGTGGAGGATTGTGAGTCTGGGCAG
  TAAATCCACAGCCAACAAACATAGAAACGACAAATCACCATGTAACAACCAGAGATGACT
  GAAACCACTCTGAAATAATGAATGTGGATAGCTGCCTTTTTTAACACTAGAAAACATTCC
  AAAACTTTAAGGTGTTGGTGTATTTGCCAGCTTTATTTGCTGTACTTTATTTGTATTTGC
  CATTCAGTCTAGCTTTTAAGTATATTTTTTTCTTTTTCTCATTTTCAATGCATATTAGTT
  TTGCATCTGTTTTGTGACCTGTTAGATGTGACACATTCTCTTTTTGTTTATTCCCTTATT
  CTAAATGAGTTCTAAAAACATAATT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000408 unedited
  NGGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTCGCTGG
  GAGCACCCGGGCCGAGGCTCTGATTCTGGGGGGAGGCCGACTCCACCCTGGCTGGAGGAA
  CTGGGTGCTCCTGCCCGCTGGCCCCTCGCGCGTGAGGATCTATCTCAGGCTAAGAAATGG
  CATTTCAAAAGGCAGTGAAAGGGACGATTCTTGTTGGAGGAGGTGCTCTTGCAACTGTTT
  TAGGACTTTCTCAGTTTGCTCATTACAGAAGGAAACAAATGAACCTGGCCTATGTTAAAG
  CAGCAGACTGCATTTCAGAACCAGTTAACAGGGAGCCTCCTTCCAGAGAAGCTCAGCTAC
  TGACTTTGCAAAACACATCTGAATTTGATATCCTTGTTATTGGAGGAGGAGCAACAGGAA
  GTGGCTGTGCGCTAGATGCTGTCACCAGAGGACTAAAAACAGCCCTTGTAGAAAGAGATG
  ATTTCTCATCAGGGACCAGCAGCAGAAGCACTAAATTGATCCATGGTGGTGTGAGATATC
  TGCAGAAGGCCATCATGAAGTTGGATATTGAGCAGTATAGGATGGTAAAAGAAGCCCTTC
  ATGAGCGTGCCAACCTGCTAGAAATTGCTCCCCATTTATCAGCTCCATTGCCTATAATGC
  TTCCAGTTTACAAGTGGTGGCAGTTACCTTACTACTGNGTAGGAATCAAGCTGTATGATT
  TGGTTGCAGGAAGCAATTGCCTAAAAAGCAGTTATGTCCTCAGCAAATCAAGAGCCCTTG
  AACATTTCCCAATGCTCCAGAAGGACANACTGGGTAGAGCAATTGTCTACTATGACGGGA
  CACATAACGATGCACGGATGAACCTTGCCATTGCTCTGACTGCTGCCCAGTATGGNGGCT
  GCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000408 unedited
  CACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCAAAAATTATGTTTTTAGAAC
  TCATTTAGAATAAGGGAATAAACAAAAAGAGAATGTGTCACATCTAACAGGTCACAAAAC
  AGATGCAAAACTAATATGCATTGAAAATGAGAAAAAGAAAAAAATATACTTAAAAGCTAG
  ACTGAATGGCAAATCTAAATAAAGTACAGCAAATAAAGCTGGCAAATACACCAACACCTT
  AAAGTTTTGGAATGTTTTCTAGTGTTAAAAAAGGCAGCTATCCACATTCATTATTTCAGA
  GTGGTTTCAGTCATCTCTGGTTGTTACATGGAGATTTGTCGTTTCTATGTTTGTTGGCTG
  TGGATTTACTGCCCAGACTCACAATCCTCCACAACTACGGTCCACTGGAATTGGAACTCT
  TCTGTCGAGGTTCTCTTCTGCAGTTTTCATTAGTATAGCAAGCCGGCTTCCAGATACCCT
  TCCTTTTTGAATAGCACTCATCAGCTGCAAAAATTCATTGAGTTCAACCTGTCCATTTTT
  ATTCAAATCAACTTCATTTAAAATCTCATGGAGTGTATTTTCATCCATTTGGACATTGAT
  ACTCTCTATACACGCTGAACATTAACAATTGGTAATAAAGCCTTTCTGGTCTGCATAAAC
  CTTATGAAATCTCTTCTTATACCTGTCAATGTCTGAAAGCAGTAAGCTAATTTCAGAGCA
  ATCTGTTAACTGTTCTGATCGAGATTATAGCCCATTTATAATAAAAAACTCCCTGGCCGT
  TTAAAGTGTTCCTGCTCTTATAAAATCCCATTCAGTCCCTGCCCTAATTCACAATCCCGG
  TAGGGCTTCTTTGTTGCCGAAATTTAAAAAGCCAGCGAGTCCGACTGAAATTTTTCCCAG
  AATGGCAGCTTCTCCTTATCCCATATCACCCTGGTTCAATATAGGAATTTGACAAAAATA
  TCTCACAAAGGCACCTTGCCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000408
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000408.2, NP_000399.1
• RefSeq Size: 2796 bp
• RefSeq ORF: 2184 bp
• Locus ID: 2820
• Cytogenetics: 2q24.1
• Domains: EFh, DAO
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: 'The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.