GPD2 (NM_000408) Human Untagged Clone

CAT#: SC119913

GPD2 (untagged)-Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 2


  "NM_000408" in other vectors (6)

Reconstitution Protocol

USD 1,470.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GPD2
Synonyms GDH2; GPDM; mGDH; mGPDH
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000408 edited
GCGGCCGCGAATTCGGCACGAGGCTCGCTGGGAGCACCCGGGCCGAGGCTCTGATTCTGG
GGGGAGGCCGACTCCACCCTGGCTGGAGGAACTGGGTGCTCCTGCCCGCTGGCCCCTCGC
GCGTGAGGATCTATCTCAGGCTAAGAAATGGCATTTCAAAAGGCAGTGAAAGGGACGATT
CTTGTTGGAGGAGGTGCTCTTGCAACTGTTTTAGGACTTTCTCAGTTTGCTCATTACAGA
AGGAAACAAATGAACCTGGCCTATGTTAAAGCAGCAGACTGCATTTCAGAACCAGTTAAC
AGGGAGCCTCCTTCCAGAGAAGCTCAGCTACTGACTTTGCAAAACACATCTGAATTTGAT
ATCCTTGTTATTGGAGGAGGAGCAACAGGAAGTGGCTGTGCGCTAGATGCTGTCACCAGA
GGACTAAAAACAGCCCTTGTAGAAAGAGATGATTTCTCATCAGGGACCAGCAGCAGAAGC
ACTAAATTGATCCATGGTGGTGTGAGATATCTGCAGAAGGCCATCATGAAGTTGGATATT
GAGCAGTATAGGATGGTAAAAGAAGCCCTTCATGAGCGTGCCAACCTGCTAGAAATTGCT
CCCCATTTATCAGCTCCATTGCCTATAATGCTTCCAGTTTACAAGTGGTGGCAGTTACCT
TACTACTGGGTAGGAATCAAGCTGTATGATTTGGTTGCAGGAAGCAATTGCCTAAAAAGC
AGTTATGTCCTCAGCAAATCAAGAGCCCTTGAACATTTCCCAATGCTCCAGAAGGACAAA
CTGGTAGGAGCAATTGTCTACTATGACGGACAACATAACGATGCACGGATGAACCTTGCC
ATTGCTCTGACTGCTGCCAGGTATGGGGCTGCCACAGCCAATTACATGGAGGTAGTGAGC
TTGCTCAAGAAGACAGACCCCCAGACAGGGAAAGTGCGTGTGAGCGGCGCACGGTGCAAG
GATGTCCTCACAGGGCAGGAATTTGACGTGAGAGCCAAATGTGTTATCAATGCCACGGGA
CCTTTCACGGACTCTGTGCGCAAAATGGATGATAAAGACGCAGCAGCTATCTGCCAGCCA
AGTGCTGGTGTCCATATTGTGATGCCTGGTTATTACAGCCCAGAGAGCATGGGACTTCTT
GACCCAGCGACCAGTGATGGGCGAGTTATTTTCTTCTTACCCTGGCAAAAGATGACGATC
GCTGGCACTACTGATACTCCAACTGATGTTACACACCATCCAATTCCTTCAGAAGAAGAT
ATCAACTTCATTTTGAATGAAGTGCGTAATTACCTGAGTTGTGATGTTGAAGTGAGAAGA
GGGGATGTCCTGGCAGCATGGAGTGGAATCCGTCCTCTTGTTACAGACCCCAAATCTGCA
GATACTCAGTCTATCTCCCGAAATCATGTTGTTGATATCAGTGAGAGTGGCCTTATTACT
ATAGCAGGTGGAAAGTGGACAACTTATCGGTCTATGGCAGAAGATACCATAAATGCTGCT
GTCAAAACTCATAATTTAAAAGCAGGACCAAGTAGAACAGTTGGGCTTTTCCTTCAAGGG
GGTAAAGATTGGAGCCCCACACTCTACATTAGGCTTGTGCAGGATTATGGACTTGAAAGC
GAGGTGGCACAGCATCTTGCCGCCACCTATGGTGATAAGGCCTTTGAGGTGGCCAAATGG
CAAGTGTGACTGGCAAAAGGTGGCCTATTGTTGGAGTACGTCTTGTGTCAGAATTTCCAT
ATATTGAAGCAGAGGTGAAATATGGGATTAAGGAGTATGCCTGCACTGCTGTGGATATGA
TTTCACGTCGTACTCGCCTGGCCTTTCTAAATGTCCAGGCAGCAGAGGAAGCCCTACCCA
GGATTGTTGAACTGATGGGCAGGGAACTGAATTGGGATGATTATAAGAAGCAGGAACAAC
TTGAAACAGCCAGGAAGTTTCTATATTATGAAATGGGCTATAAATCTCGATCAGAACAGT
TAACAGATCGCTCTGAAATTAGCCTACTGCCTTCAGACATTGACAGGTATAAGAAGAGAT
TTCATAAGTTTGATGCAGACCAGAAAGGCTTTATTACCATTGTTGATGTTCAGCGTGTAT
TAGAGAGTATCAATGTCCAAATGGATGAAAATACACTCCATGAAATTCTAAATGAAGTTG
ATTTGAATAAAAATGGACAGGTTGAACTCAATGAATTTTTGCAGCTGATGAGTGCTATTC
AAAAAGGAAGGGTATCTGGAAGCCGGCTTGCTATACTAATGAAAACTGCAGAAGAGAACC
TCGACAGAAGAGTTCCAATTCCAGTGGACCGTAGTTGTGGAGGATTGTGAGTCTGGGCAG
TAAATCCACAGCCAACAAACATAGAAACGACAAATCACCATGTAACAACCAGAGATGACT
GAAACCACTCTGAAATAATGAATGTGGATAGCTGCCTTTTTTAACACTAGAAAACATTCC
AAAACTTTAAGGTGTTGGTGTATTTGCCAGCTTTATTTGCTGTACTTTATTTGTATTTGC
CATTCAGTCTAGCTTTTAAGTATATTTTTTTCTTTTTCTCATTTTCAATGCATATTAGTT
TTGCATCTGTTTTGTGACCTGTTAGATGTGACACATTCTCTTTTTGTTTATTCCCTTATT
CTAAATGAGTTCTAAAAACATAATT
>OriGene 5' read for NM_000408 unedited
NGGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTCGCTGG
GAGCACCCGGGCCGAGGCTCTGATTCTGGGGGGAGGCCGACTCCACCCTGGCTGGAGGAA
CTGGGTGCTCCTGCCCGCTGGCCCCTCGCGCGTGAGGATCTATCTCAGGCTAAGAAATGG
CATTTCAAAAGGCAGTGAAAGGGACGATTCTTGTTGGAGGAGGTGCTCTTGCAACTGTTT
TAGGACTTTCTCAGTTTGCTCATTACAGAAGGAAACAAATGAACCTGGCCTATGTTAAAG
CAGCAGACTGCATTTCAGAACCAGTTAACAGGGAGCCTCCTTCCAGAGAAGCTCAGCTAC
TGACTTTGCAAAACACATCTGAATTTGATATCCTTGTTATTGGAGGAGGAGCAACAGGAA
GTGGCTGTGCGCTAGATGCTGTCACCAGAGGACTAAAAACAGCCCTTGTAGAAAGAGATG
ATTTCTCATCAGGGACCAGCAGCAGAAGCACTAAATTGATCCATGGTGGTGTGAGATATC
TGCAGAAGGCCATCATGAAGTTGGATATTGAGCAGTATAGGATGGTAAAAGAAGCCCTTC
ATGAGCGTGCCAACCTGCTAGAAATTGCTCCCCATTTATCAGCTCCATTGCCTATAATGC
TTCCAGTTTACAAGTGGTGGCAGTTACCTTACTACTGNGTAGGAATCAAGCTGTATGATT
TGGTTGCAGGAAGCAATTGCCTAAAAAGCAGTTATGTCCTCAGCAAATCAAGAGCCCTTG
AACATTTCCCAATGCTCCAGAAGGACANACTGGGTAGAGCAATTGTCTACTATGACGGGA
CACATAACGATGCACGGATGAACCTTGCCATTGCTCTGACTGCTGCCCAGTATGGNGGCT
GCA
>OriGene 3' read for NM_000408 unedited
CACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCAAAAATTATGTTTTTAGAAC
TCATTTAGAATAAGGGAATAAACAAAAAGAGAATGTGTCACATCTAACAGGTCACAAAAC
AGATGCAAAACTAATATGCATTGAAAATGAGAAAAAGAAAAAAATATACTTAAAAGCTAG
ACTGAATGGCAAATCTAAATAAAGTACAGCAAATAAAGCTGGCAAATACACCAACACCTT
AAAGTTTTGGAATGTTTTCTAGTGTTAAAAAAGGCAGCTATCCACATTCATTATTTCAGA
GTGGTTTCAGTCATCTCTGGTTGTTACATGGAGATTTGTCGTTTCTATGTTTGTTGGCTG
TGGATTTACTGCCCAGACTCACAATCCTCCACAACTACGGTCCACTGGAATTGGAACTCT
TCTGTCGAGGTTCTCTTCTGCAGTTTTCATTAGTATAGCAAGCCGGCTTCCAGATACCCT
TCCTTTTTGAATAGCACTCATCAGCTGCAAAAATTCATTGAGTTCAACCTGTCCATTTTT
ATTCAAATCAACTTCATTTAAAATCTCATGGAGTGTATTTTCATCCATTTGGACATTGAT
ACTCTCTATACACGCTGAACATTAACAATTGGTAATAAAGCCTTTCTGGTCTGCATAAAC
CTTATGAAATCTCTTCTTATACCTGTCAATGTCTGAAAGCAGTAAGCTAATTTCAGAGCA
ATCTGTTAACTGTTCTGATCGAGATTATAGCCCATTTATAATAAAAAACTCCCTGGCCGT
TTAAAGTGTTCCTGCTCTTATAAAATCCCATTCAGTCCCTGCCCTAATTCACAATCCCGG
TAGGGCTTCTTTGTTGCCGAAATTTAAAAAGCCAGCGAGTCCGACTGAAATTTTTCCCAG
AATGGCAGCTTCTCCTTATCCCATATCACCCTGGTTCAATATAGGAATTTGACAAAAATA
TCTCACAAAGGCACCTTGCCAAT
Restriction Sites NotI-NotI     
ACCN NM_000408
Insert Size 2870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000408.2, NP_000399.1
RefSeq Size 2796 bp
RefSeq ORF 2184 bp
Locus ID 2820
Cytogenetics 2q24.1
Domains EFh, DAO
Protein Families Druggable Genome, Transmembrane
Protein Pathways Glycerophospholipid metabolism
Gene Summary 'The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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