PEX5 (NM_000319) Human Untagged Clone

CAT#: SC119969

PEX5 (untagged)-Human peroxisomal biogenesis factor 5 (PEX5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEX5
• Synonyms: PBD2A; PBD2B; PTS1-BP; PTS1R; PXR1; RCDP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000319, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAATGCGGGAGCTGGTGGAGGCCGAATGCGGGGGTGCCAACCCGCTCATGAAGCTCGCCGGGCACT
  TCACCCAGGACAAGGCCCTTCGGCAGGAGGGATTGAGGCCTGGCCCCTGGCCCCCCGGAGCCCCGGCCTC
  TGAGGCAGCCTCCAAGCCTTTGGGAGTAGCTTCTGAAGATGAGTTGGTGGCTGAATTCCTGCAGGACCAG
  AATGCACCCCTTGTGTCCCGTGCCCCTCAGACCTTCAAGATGGATGACCTCCTGGCTGAGATGCAGCAGA
  TTGAGCAGTCAAACTTCCGCCAGGCTCCCCAGAGAGCCCCTGGTGTGGCAGACTTGGCCTTGTCTGAGAA
  CTGGGCCCAGGAGTTTCTTGCAGCTGGAGATGCTGTGGATGTAACTCAGGATTATAATGAGACTGACTGG
  TCCCAAGAATTCATCTCTGAAGTTACAGACCCCTTGTCTGTGTCCCCTGCCCGCTGGGCTGAGGAATATT
  TGGAGCAATCAGAGGAGAAGCTGTGGCTGGGAGAACCTGAGGGAACAGCCACCGATCGCTGGTATGATGA
  ATATCATCCTGAGGAGGATCTGCAGCACACGGCCAGTGACTTTGTGGCCAAAGTGGATGACCCCAAATTG
  GCTAATTCTGAGTTCCTGAAATTCGTGCGGCAGATTGGCGAAGGGCAGGTGTCCCTGGAGTCCGGTGCAG
  GGTCGGGCCGAGCTCAGGCAGAACAGTGGGCAGCAGAGTTTATACAGCAGCAGGGTACATCAGATGCCTG
  GGTTGACCAGTTCACAAGACCAGTAAACACATCTGCCCTTGATATGGAGTTTGAACGAGCCAAGTCAGCT
  ATAGAGTTGCAGGCAGAGTTGGAGGAGATGGCAAAACGGGATGCTGAGGCCCACCCCTGGCTTTCTGACT
  ATGATGACCTTACGTCAGCTACCTATGATAAGGGGTACCAGTTTGAGGAGGAGAACCCCTTGCGTGATCA
  CCCTCAGCCTTTTGAAGAAGGGCTGCGGCGCCTTCAGGAGGGGGACCTGCCAAATGCTGTGCTGCTTTTT
  GAGGCAGCTGTGCAGCAGGATCCTAAGCACATGGAAGCTTGGCAGTATCTGGGTACCACCCAGGCAGAGA
  ATGAACAAGAACTATTAGCCATCAGTGCATTGCGGAGGTGTCTGGAGCTAAAGCCAGATAACCAGACAGC
  ACTGATGGCGCTGGCTGTGAGCTTCACCAACGAGTCCCTGCAGCGACAGGCCTGTGAAACCCTACGAGAC
  TGGCTGCGGTACACACCAGCCTATGCCCATCTGGTGACACCTGCTGAAGAAGGGGCTGGTGGGGCAGGAC
  TGGGCCCCAGCAAGCGTATCCTGGGATCTCTCTTGTCTGACTCCCTGTTTCTTGAAGTGAAAGAGCTCTT
  CCTGGCAGCTGTGCGGCTGGACCCTACCTCCATTGACCCTGATGTGCAGTGTGGCTTGGGAGTCCTTTTC
  AACCTGAGTGGGGAGTATGACAAGGCCGTGGACTGCTTCACAGCTGCCCTCAGCGTTCGTCCCAATGACT
  ATTTGCTGTGGAATAAGCTAGGCGCCACCCTGGCCAATGGAAACCAGAGTGAAGAAGCAGTAGCTGCGTA
  CCGCCGGGCCCTCGAGCTCCAGCCTGGCTATATCCGGTCCCGCTATAACCTGGGCATCAGCTGCATCAAC
  CTCGGGGCTCACCGGGAGGCTGTGGAGCACTTTCTGGAGGCCCTGAACATGCAGAGGAAAAGCCGGGGCC
  CCCGGGGTGAAGGAGGTGCCATGTCGGAGAACATCTGGAGCACCCTGCGTTTGGCATTGTCTATGTTAGG
  CCAGAGCGATGCCTATGGGGCAGCCGACGCGCGGGATCTGTCCACCCTCCTAACTATGTTTGGCCTGCCC
  CAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000319 unedited
  GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCCGGGGTCCAG
  GCCCCTTTGTGGAGGCAGCTGCTGGCCCCCAGCCCATGGGACCGTAGTCGCGGAGGCCTC
  TGCAGAGGCGCAGGCTGGAAGCGGTGGCCTTTGAGGGGGGCGGCAGGAGAGAGTACCGAC
  CTCCCTCGAACTCCTGGCAGAGAGAGCTGGCGGTCACCATGGCAATGCGGGAGCTGGTGG
  AGGCCGAATGCGGGGGTGCCAACCCGCTCATGAAGCTCGCCGGGCACTTCACCCAGGACA
  AGGCCCTTCGGCAGGAGGGATTGAGGCCTGGCCCCTGGCCCCCCGGAGCCCCGGCCTCTG
  AGGCAGCCTCCAAGCCTTTGGGAGTAGCTTCTGAAGATGAGTTGGTGGCTGAATTCCTGC
  AGGACCAGAATGCACCCCTTGTGTCCCGTGCCCCTCAGACCTTCAAGATGGATGACCTCC
  TGGCTGAGATGCAGCAGATTGAGCAGTCAAACTTCCGCCAGGCTCCCCAGAGAGCCCCTG
  GTGTGGCAGACTTGGCCTTGTCTGAGAACTGGGCCCAGGAGTTTCTTGCAGCTGGAGATG
  CTGTGGATGTAACTCAGGATTATAATGAGACTGACTGGTCCCAAGAATTCATCTCTGAAG
  TTACAGACCCCTTGTCTGTGTCCCCTGCCCGCTGGGCTGAGGAATATTTGGAGCAATCAG
  AGGAGAAGCTGTGGCTGGGAGAACCTGAGGGAACAGCCACCGATCGCTGGTATGATGAAT
  ATCATCCTGAGGAGGATCTGCAGCACACGGGCCAGTGACTTTGTGGCCAAAGTGGATGAC
  CCCCAATGGGCTATTCTNGAGGACATCANATGCCTGGGTGACCGNTCACAAGACGTAACA
  CTCTGCCTTGATATGGAGTTGACGAGCAGTCACTTAGATCTGAGTCGATTCTGGGACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000319 unedited
  NCCCTCGGTGNACCCGGCCGCAATCNANGATCGAGNTTTTTTTTTTTTTTTTTTTACGTT
  TTTTTTTTTTTTTTTTTTTTTTTTTCCCACATAAGTTCCTTTTATTTTTCGAACAAAATT
  TTAACAGTTTTATTTTTAAATCTTTAACCCTTTTTCTTACAAAAAAAAATAATTTTCAGG
  ATGGAATCCTGGGCCCATGGAAGTTAAAAAAAAAAATTTTTTTGATCTTTACCTCCTAAA
  AACAAGGCAAGAGGCTTACCAGCAATTTCTGGGGGTTAGGGTTTCCCCCCATGCGGGGGA
  CACTGGAAAAAATCCCAAGCGTGCCCCTCATTTTCCTTCAACCCAAAGGGGGGAAGGGGA
  TTTTCACATGCCTTCCCTTAAAAACCTTCCCCAAAACACTCACTAAGGGTTTAGGGCACC
  CCTGACCCTTTCCGCCTACAACTTGGCACCGATGCGCTCTCCTCCCACCCCTTCTCTCTT
  CCCTGCTGCCTTATCACACGTTTTCCCCTTTCTTCTCTCCAGCTCTCCCGCATTCCCCCC
  CCTCTCCTCTACTCTCCGCCCCCCTCCCTCCGCTCCTTCCCCCCCCCCCGTCCTTACTCA
  TCGCTCTTGCCCCCCCATTCCGCTCCACCTGACCCCCGCATCTCTCTCTTAGCCACCTCC
  CCTCCTAACCCCTCCATCCCCCGACCCTATCCCCGGTCCTCTCTACCTTGCCATTCTCCC
  CCCTTCCTCACGTCCTCCTCCCTCACGTCCTCCAAACCCTCCCTTCCCCCCACCGTACTC
  ATTTATTCCGGTTGTATATATTCCCACCCTCATCCCCGCACTCAACCATATCCACCTAAA
  CATCCTCTCTTCCTCCTCCCCCCCTCTCTCATATCACATCCCTCTCCCTCCCACCTTTTG
  TTATATGTCATCTCTCTCCTGACACCCACTATCCTTTTAATTCGTAGTTCCCTTCTCCCT
  CCCCTCTCTATATCTCCTTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000319
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000319.3, NP_000310.2
• RefSeq Size: 3215 bp
• RefSeq ORF: 1896 bp
• Locus ID: 5830
• Cytogenetics: 12p13.31
• Domains: TPR
• Protein Families: Druggable Genome
• Gene Summary: 'The product of this gene binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of neonatal adrenoleukodystrophy (NALD), a cause of Zellweger syndrome (ZWS) as well as may be a cause of infantile Refsum disease (IRD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR, initiates translation from a downstream start codon, and uses an alternate splice site in the central coding region, compared to variant 6. The encoded isoform (b) has a shorter N-terminus compared to isoform e.